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- Feb 2023
Tissues were then frozen in optimum cutting temperature embedding medium and cryosectioned for direct GFP visualization (brain was sectioned at 30 μm, and other tissues at 8 μm). Images were acquired with a Nikon Eclipse Ti-E fluorescence microscope.
I was initially critical of the sample size they described earlier but reading through this specific protocol makes it seem extremely tedious to collect data. Has anyone here performed these types of studies on neurons and/or brain tissue? How tedious does this get?