because of its small size, binding of tris-NTA has minimum effect on biochemical and physicochemical properties of the protein
small tag may not alter the conformation and function of the protein
2 Matching Annotations
- Sep 2021
-
Local file Local file
-
-
The observed Kd values were 1.3 ± 0.2 nM for the His6 peptide, 0.6 ± 0.3 nM for IDH R132H and 2.4 ± 1.1 nM for p38α
2 points:
- binding Kd is about 1nM
- Kd for different targets could be slightly different
-