Its efficacy has not been proven for the treatment of occipital neuralgia or complex regional pain syndrome.
This is one of those treatments where the BoNT comes from matters, or whether the toxin will spread. The wrong dealer/batch or the 'spread of the toxin' will cause permanent neurological issues. So, this is one clinical trial that can make one's current condition much worse. Is it worth pursuing any further?
hus, the negative results of these studies might be influenced by the mode of administration.
Would be interesting to see how these results on Carpal Tunnel Syndrome compares to osteopathic manipulation treatment.
A potential confound in the procedures, however, is that in the first study [126], mice in the exercise condition also received extra environmental enrichment (larger cage space, variety of additional objects rotated regularly), and in the second study [127], mice in the environmental enrichment condition also received a running wheel. So it is not clear whether exercise or environmental enrichment alone is responsible for the improved learning in either study, or to what degree each treatment contributed to the improvements observed in these groups.
Proper nutrition AND exercise is the key; not one or the other. Docs use to believe if you exercised well [however they define well], you can eat anything. Now of course, that is nonsense. Both are critical, why docs should be pushing this, as gov-associated docs (like Surgeon General) will never have the courage to speak out about Fast Foods because lobbyists not scientists dictate healthcare/nutrition policies. It's sad, but it's why healthcare providers need to sound like a broken record and stress proper nutrition and exercise. Granted, nutritional studies are like politics, everyone has an opinion [or paid opinion]. The politics here is similar to the politics of global warming; but unlike the global warming politics, people DO have a direct impact on their own health with proper nutrition and exercise.
Future studies using a variety of AD mouse models should investigate the effects of diet reversal following long-term HFD consumption on markers of inflammation in brain and peripheral tissues (fat, liver, etc.).
No...disagree. This is not an experimental drug, not a gene therapy process, and this is not an surgical procedure where 'future studies' [more and more studies] or long-term studies are critical. Furthermore, in this case, animal studies are a waste [reading the book, NEUROLOGY OF BRAIN DISORDERS, shows that animal studies yield nothing in human trials]. Habits in eating and exercise can save millions NOW, as many don't have 25 years to wait for the obvious. Obvious = fruits/vegetables/drinking plenty of clean water is 100x better than fast food.
Recent research suggests that there are stronger similarities between AD and T2DM than have previously been considered.
Alzheimer's Disease = Diabetes Type II of the brain? Absolutely!
Nice! What this equals is: proper nutrition + exercise = reducing [perhaps reversing] cognitive decline.
Not surprisingly, these clinical diseases further illustrate the vital role played by gut microbiota in the human body, both through healthy, beneficial states and, more importantly, in altered, dysfunctional states
Clearly, the entire field of Toxicology needs to be re-evaluated to determine the effects of compounds on microbial species residing in the human gut.
A recent review paper considering the role of gut microbiota in the etiology of ASD found that many studies have indeed identified the microbiota composition in ASD subjects to differ from those in healthy controls
This may help to explain the effects of the autism diet, which eliminates wheat and dairy and is very popular in europe.
is a neuropsychiatric syndrome associated with altered gut microbiota
I recently read about the altered osmotic pressure in astrocytes that is also caused by this syndrome
commonly linked with the well-being of mood
healthy = happy and happy = healthy
autonomic and enteric nervous system
I think that the enteric system is part of the autonomic nervous system...
the ability of gut microbiota to bidirectionally communicate with the brain
It is not only the microbiota, but the nervous system embedded within the gut that communicates to the brain
fecal microbiota transplants
I totally cannot get over the yuck factor of this... coming soon~ poop in a pill~!
Important to keep in mind the causation vs. correlation fallacy here
Dash S, Clarke G, Berk M, Jacka FN. The gut microbiome and diet in psy-chiatry: focus on depression. Curr Opin Psychiatry. 2015;28(1):1–6
This could be a novel way of addressing mental illness... especially in psychiatric hospitals. The diet fed to the patients may need to be adjusted to promote a healthy gut microbiome as part of the therapy in combating depression.... Having worked in a psych hospital I can speak first hand about how institutional the food is.
Cryan JF, Dinan TG. Mind-altering microorganisms: the impact of the gut microbiota on brain and behaviour. Nat Rev Neurosci. 2012;13(10):701–712
with exposure to more dangerous microorganisms how does this affect what we know of as the microbiome? Staph is a common bacteria that resides in healthcare workers noses from common exposure....I wonder if healthcare workers gut microflora is different then the general population
Alistipes levels and other gut microbiota can be modified through dietary intervention,58 which may prove as an influential intervention for depressive disorders
Once again diet place a key role in reaching optimum health/ disease free state of being
GF mice have no commensal intestinal microbiota and as such exhibit an undeveloped immune system
The microbiome from the gut has been shown to be very important in newborns. As the baby passes through the birth canal-through a vaginal birth it is exposed to the mothers good bacteria and helps establish their immune system. A few studies have shown c-section babies have lower immune systems because of the possible lack of exposure to good bacteria from Mom. So, this was not a surprising statement.
altered microbiota has been linked to neuropsychological disorders including depression and autism spectrum disorder, metabolic disorders such as obesity, and gastrointestinal disorders including inflammatory bowel disease and irritable bowel syndrome.
A lot of illnesses that we have not been able to pinpoint exact causes for. Another theory to give us a hopeful answer to some debilitating ailments
1014 gut microbiota and composed of over a thousand species
An impressive symbiotic environment
1014 gut microbiota and composed of over a thousand species
Clear symbiotic relationship
Keywords: gut microbiota, mental illness, disease, modulation, therapy, probiotics
Each keyword forms an important concept, which is explained in this chapter at some point. Initially the author explains the concept of probiotic organisms (bacteria that promote digestive health and improve immune system of a person) and gives some examples of these gut microorganisms (e.g. Lactobacillus bulgaricus, Lactococcus lactis, etc.). The author then moves on to explain role of these bacteria in communicating with brain and regulating various diseases like anxiety, depression(mental illness), metabolic and GI tract disorders. Finally the author assesses the potential of modifying these bacterial communities to treat these disorders. Thus, all keywords play an important role in introducing various important concepts of the chapter.
The gut–brain axis in disease
I have always been interested in studying the pathways that link various biochemical processes to diseases. I believe that if we could understand these pathways, then it is possible to modify them to improve human health and reduce the occurrence/risk of or treat various diseases. Bidirectional communication between gut microbiome and brain has opened new doors for the betterment of human health. These microorganisms regulate many complex behaviors such as anxiety, learning, memory and appetite. Recent studies show that our diet can be modified to treat various disorders such as anxiety, depression, metabolic disorders like PKU and gastrointestinal tract disorders.
Goals of the paper: 1) To introduce the concept of bidirectional communication between brain and gut to the audience 2) To demonstrate the role of the bidirectional communication in regulating various disorders 3) To analyze the potential of modulation of gut microbiome to treat various disorders and promote human health
The above-mentioned goals were very clear from author's choice of topics and language used in the article. The author explains all concepts in a simple, layman's language, which can be understood by general public and the content can be easily related to general day-to-day concept of disease and its treatment.
Modulation of microbiota and prospects for therapy
That will provide us with an exciting opportunity to treat some complex disorders. I found one TED talk, which relates gut microbiome with various disorders.
Luna RA, Foster JA. Gut brain axis: diet microbiota interactions and implications for modulation of anxiety and depression. Curr Opin Biotechnol. 2014;32C:35–41
That would take anxiety and depression research to the next level and will really help patients with anxiety disorders such as autism.
Husebye E, Hellstrom PM, Midtvedt T. Intestinal microflora stimulates myoelectric activity of rat small intestine by promoting cyclic initiation and aboral propagation of migrating myoelectric complex. Dig Dis Sci. 1994;39(5):946–956
Wow! Is it possible even in humans??
Diamant M, Blaak EE, de Vos WM. Do nutrient-gut-microbiota interactions play a role in human obesity, insulin resistance and type 2 diabetes? Obes Rev. 2011;12(4):272–281.
This would be very interesting article to read and it might give us useful insights regarding the effect of interactions between gut bacteria and nutrients on obesity and type 2 diabetes.
Interestingly, the modulation of microbiota has been suggested as preventative against the common cold and influenza-like symptoms in children.98 In a double-blind, placebo-controlled study, 326 children aged between 3years and 5years were randomly assigned to receive Lactobacillus acidophilus (n=110), L. acidophilus and B. animalis lactis Bi-07 (n=112), or a placebo (n=104), treated twice daily for 6 months. At the end of the study, it was found that relative to the placebo group, those who received single and combi-nation probiotics reduced their incidences and duration of fever, coughing, and rhinorrhea significantly.
This can be valuable with over-the-counter products; though, the DIETARY ACT OF 1994 can be dangerous here. Good thing ConsumerLab.com tries to keep on eye on such things.
Abstract:
Antibiotics can play a bigger role than expected: "A new study shows that people who take multiple courses of antibiotics are at increased risk for both type 1 and type 2 diabetes, most likely because antibiotics change the bacteria in their guts (European Journal of Endocrinology, published online March 24, 2015)." The pieces of the 10,000-piece puzzle are coming together nicely...so far.
Concluding statement
Adding to the concluding statement [from http://drmirkin.com/diabetes/antibiotics-may-increase-diabetes-risk.html]:
" Other studies in both humans and animals have shown that: • Early childhood exposure to antibiotics is associated with obesity in later life. • People with diabetes have different bacteria in their guts from those who do not have that disease. • Fecal transplants from obese mice cause thin mice to become fat. • Putting thin mice on antibiotics can make them fat. These and many other studies suggest that taking antibiotics is associated with changes in the bacteria in your gut that can increase risk for obesity, diabetes and inability to respond to insulin."
person’s microbial profile is distinct
Could this be a future diagnostic tool. Using super computers on individual microbial gut profile? More accurate for immediate and future health than genetic profiling?
sacrificed
I am surprised the word 'sacrificed' is still used, as to imply some secret religious ceremony that is part of the experimental protocol. Why not just use 'kill'? Sacrifice implies the transforming of a victim into both an object and a myth.
This reduction was not observed after treatment with PBM. A significant effect was only observed with the fluence rate of 5mW/cm2 (## p<0.01, Tukey’s test).
The p-value is statistically significance, but I see the ranges to be similar. So if I was to do a blind analysis on this it would be all over, and would be just guessing if I had to choose one.
Statistical analysis
Why wasn't a quantile-quantile plot first done? "Many statistical procedures, including t-test, as it is standardly used, are valid only when the points of the empirical quantile-quantile plot are parallel to the line y = x." SCIENCE [1984 Oct 12;226(4671):156-7]. An article often ignored but useful.
Sham illumination was performed by placing the animal in the same cylinder with the light turned off.
Good and simple process to do to ensure the illumination data is the illumination data and not a cylinder-effect [chamber].
No significant difference in the levels or pattern of human α-syn expression between the different experimental conditions was observed.
That is also what I see looking at this...no 'observational stretching' here.
overexpression of α-syn
Why an over-expression of alpha syn? Is it a brain protective mechanism causing PD as a side-effect, or a damaging effect that started upstream causing PD?
Moreover, our data demonstrate that brief and chronic exposure to NIR light induced a beneficial effect when applied during the early stages of the disease, suggesting that this treatment could interfere with the primary events underlying the initiation of PD and related synucleinopathies.
"...during the early stages of the disease..." is of course beneficial for any disease but super hard to define in all brain-related diseases. What is truly early in Parkinson's Disease that all docs would agree to that 'early' treatment? Otherwise, early is arbitrary and so are the results from any treatment.
Photo-Induced Cross-linking of Unmodified Proteins (PICUP)
Here is a YouTube video [4:15 min] showing both the PICUP and the SDS PAGE in a laboratory: https://www.youtube.com/watch?v=MqQtlYJrrDc
These observations highlight the importance of defining the structures and properties of the various species involved in Aβ aggregation. Indeed, it is only through this level of molecular detail that we will be able to interfere with Aβ aggregation in a rational manner.
This reminds me of how physicists study crystal structure/structure formation under many conditions. It's a good strategy neuroscientists [really, biomedical researchers] should think as well with respect to in-vitro experimentation = "what happens to this protein when....and how does it relate to the projected in-vivo model, and perhaps is that model flawed?"
ESI-IM-MS
A tool used to characterize cellular function...made popular because of the Human Genome Project with respect to protein identification/characterization. Here is a good paper on explaining the use of mass spec (the MS part of the ESI-IM-MS) with proteins: SPECTROSCOPY 16(2002) 15-28. https://masspec.scripps.edu/publications/public_pdf/78_art.pdf It's 10+ years-old, but it's still good.
For example, according to the Web of Science, there are six key papers7,8,9,16,17,18, frequently cited together (more than one thousand times), that constitute the foundational core for the research front entitled “Aβ oligomers, fibrils and AD”. Three of these six papers are based directly16,18 or indirectly7 on the conclusions derived from PICUP/SDS-PAGE analysis. However, SDS-PAGE analysis might be biased since SDS has been suggested to affect the oligomerization state of Aβ, especially that of Aβ428,19,20.
The most important and often ignore in research training [mostly in the classroom setting] is the de-emphasis of the METHODS & MATERIALS (M&M) section. M&M = why x is in the buffer or why test y is used. Stay away from the "most cited protocols," and focus on your why x or y when putting together protocols for an experiment. This becomes easier to do with more and more hands-on diverse experiences.
Figure 1: PICUP and SDS-PAGE analysis apparently indicate that Aβ40 and Aβ42 oligomerize through distinct pathways.
Figure 1 is a really good example of how to incorporate a lab result (e.g., a gel) with a drawing of what one is most likely 'seeing.' Something I have not thought of, but I will incorporate this in my future work because this is useful for students and researchers alike.
SDS-PAGE analysis of Aβ oligomers is disserving research into Alzheimer´s disease: appealing for ESI-IM-MS
This publication is another reason why it's important to know as much as possible--to include obtaining hands-on experience--with the techniques used in a variety of fields. Could this help explain the disconnect between animal models and human clinical trials? I think so.