2 Matching Annotations
- May 2019
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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The colonies to be tested were streaked on the surface of minimal A-glucose plates containing either 0.4-0.7 M NaCl with 1 mM glycine betaine, and incubated at 37oC. NaCl-tolerant strains grew toform single colonies in 36-60 hrs whereas NaCl-sensitive ones did not. As controls, MC4100 (WT) and other previously identified NaCl sensitive mutants were streakedfor comparison
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NaCl-sensitivity testing
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