On 2017 Jul 13, WALTER LUKIW commented:

As originally reported by the authors, we find miRNA-168a abundant in dietary plant(s) and in the systemic circulation (and other really, really, really interesting places) of animals who consume these plants as part of their diet (unpublished observations); in our hands RT-PCR is a very easy but very inconsistent method to detect ANY type of microRNA abundance anywhere from any biological fluids or tissues, either plant or animal; we use RNA-sequencing, miRNA array-based (microfluidic hybridization) approaches, LED-Northerns, regular Northern dot blots and yes, RT-PCR - although RT-PCR approaches clearly yield the most inconsistent results.

Walter J. Lukiw BS, MS, PhD Professor of Neurology, Neuroscience and Ophthalmology Bollinger Professor of Alzheimer’s disease (AD) LSU Neuroscience Center, Louisiana State University Health Sciences Center 2020 Gravier Street, Suite 904 New Orleans LA 70112 USA TEL 504-599-0842 EMAIL wlukiw@lsuhsc.edu

On 2017 Jul 13, WALTER LUKIW commented:

As originally reported by the authors, we find miRNA-168a abundant in dietary plant(s) and in the systemic circulation (and other really, really, really interesting places) of animals who consume these plants as part of their diet (unpublished observations); in our hands RT-PCR is a very easy but very inconsistent method to detect ANY type of microRNA abundance anywhere from any biological fluids or tissues, either plant or animal; we use RNA-sequencing, miRNA array-based (microfluidic hybridization) approaches, LED-Northerns, regular Northern dot blots and yes, RT-PCR - although RT-PCR approaches clearly yield the most inconsistent results.

Walter J. Lukiw BS, MS, PhD Professor of Neurology, Neuroscience and Ophthalmology Bollinger Professor of Alzheimer’s disease (AD) LSU Neuroscience Center, Louisiana State University Health Sciences Center 2020 Gravier Street, Suite 904 New Orleans LA 70112 USA TEL 504-599-0842 EMAIL wlukiw@lsuhsc.edu