On 2014 Nov 24, Markus Meissner commented:

We characterised in this paper several conditional knockout mutants for core components of the known invasion machinery (Act1, MyoA, GAP45, MLC1 and MyoA,B/C). Surprisingly parasites remained invasive, albeit at reduced levels and for some factors, such as myosins we speculated that some functional overlap between MyoA and MyoC exists, since simultaneous depletion of these myosins resulted in a significantly stronger egress and invasion phenotype. A new study by Frenal et al., 2014 (http://www.ncbi.nlm.nih.gov/pubmed/25393004) reproduces the phenotypic consequences of the simultaneous depletion of MyoA and MyoC and provides a mechanistic explanations for these findings.

However, the central question regarding mechanistic role of the myosin/actin system during motility and invasion requires further analysis to answer the most intriguing question: How can the parasite move and invade in absence of a functional IMC (as seen in case of gap45KO parasites) or in absence of both the MyoA and MyoC motor (as seen for the MyoA,B,C mutants or in case of mlc1KO)?

On 2014 Nov 24, Markus Meissner commented:

We characterised in this paper several conditional knockout mutants for core components of the known invasion machinery (Act1, MyoA, GAP45, MLC1 and MyoA,B/C). Surprisingly parasites remained invasive, albeit at reduced levels and for some factors, such as myosins we speculated that some functional overlap between MyoA and MyoC exists, since simultaneous depletion of these myosins resulted in a significantly stronger egress and invasion phenotype. A new study by Frenal et al., 2014 (http://www.ncbi.nlm.nih.gov/pubmed/25393004) reproduces the phenotypic consequences of the simultaneous depletion of MyoA and MyoC and provides a mechanistic explanations for these findings.

However, the central question regarding mechanistic role of the myosin/actin system during motility and invasion requires further analysis to answer the most intriguing question: How can the parasite move and invade in absence of a functional IMC (as seen in case of gap45KO parasites) or in absence of both the MyoA and MyoC motor (as seen for the MyoA,B,C mutants or in case of mlc1KO)?