On 2017 Jul 17, George McNamara commented:
more nuanced review at https://www.ncbi.nlm.nih.gov/pubmed/28481306
and compelling blog and Perspective blasting MTH1:
Blagg & Workman 2017 Cancer Cell (open access) http://dx.doi.org/10.1016/j.ccell.2017.06.005
On 2016 Aug 25, Morten Oksvold commented:
In figure 3E the micrograph of merged KI67 and DAPI staining does not correspond with the single DAPI staining.
A similar problem is also seen in figure 4D, where the PI-positive cells do not correspond with the hoechst stained cells. This is seen when you adjust the brightness. In this experiment, cells are exposed to 5 mg/ml PI for 30 min. The usual is to use 1 microgram/ml and study the cells directly, otherwise the PI will also stain the live cells.
On 2016 Aug 25, Morten Oksvold commented:
In figure 3E the micrograph of merged KI67 and DAPI staining does not correspond with the single DAPI staining.
A similar problem is also seen in figure 4D, where the PI-positive cells do not correspond with the hoechst stained cells. This is seen when you adjust the brightness. In this experiment, cells are exposed to 5 mg/ml PI for 30 min. The usual is to use 1 microgram/ml and study the cells directly, otherwise the PI will also stain the live cells.
On 2017 Jul 17, George McNamara commented:
more nuanced review at https://www.ncbi.nlm.nih.gov/pubmed/28481306
and compelling blog and Perspective blasting MTH1:
Blagg & Workman 2017 Cancer Cell (open access) http://dx.doi.org/10.1016/j.ccell.2017.06.005