On 2017 Oct 12, Takashi Shichita commented:
As the comment on PubMed Common, the targeted site of our guide RNA2 was split over Exons 2 and 3. This is our mistake. However, we successfully obtained the Msr1-deficient RAW cell clone through limiting dilution. Our guide RNA1 is thought to function correctly for the disruption of Msr1 gene.
On 2017 Oct 09, Nirajkumar Makadiya commented:
Hi Authors,
Have some questions regarding the methods section of PMID: 28394332. 1) Guide RNA 2 (5′-CTTCCTCACAGCACTAAAAA-3′) for the CRISPR is spanning over exon 2 and 3. Was wondering if that can work? 2) Did you try the other sgRNA sequences to knock out the Msr1 gene?
Thank you!
On 2017 Oct 09, Nirajkumar Makadiya commented:
Hi Authors,
Have some questions regarding the methods section of PMID: 28394332. 1) Guide RNA 2 (5′-CTTCCTCACAGCACTAAAAA-3′) for the CRISPR is spanning over exon 2 and 3. Was wondering if that can work? 2) Did you try the other sgRNA sequences to knock out the Msr1 gene?
Thank you!
On 2017 Oct 12, Takashi Shichita commented:
As the comment on PubMed Common, the targeted site of our guide RNA2 was split over Exons 2 and 3. This is our mistake. However, we successfully obtained the Msr1-deficient RAW cell clone through limiting dilution. Our guide RNA1 is thought to function correctly for the disruption of Msr1 gene.