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    1. D. Secondary antibodies used for immunoblotting or confocal imaging.Figure S4Figure S4. The selected pS129 antibodies recognize all specifically pS129-aSyn but notunmodified aSyn (related to Figure 2)A. Forty nanograms of human recombinant monomeric aSyn unphosphorylated (WT) or phosphorylatedat the S129 residue (pS129) were detected by WB using commercial antibodies (pSyn#64, MJF-R13,81a, EP1536Y and GTX) or the LASH-EGT homemade pS129 antibody (respective epitopes listed inFigure S3) combined with total aSyn antibodies (Fl-140 or SYN-1).B-C. Forty nanograms of human recombinant monomeric aSyn (WT) or phosphorylated at the Y39(pY39) or S87 (pS87) or S129 residues (pS129) and WB was performed using the pY39 antibody (B)

      DOI: 10.1101/2022.03.30.486322

      Resource: (Jackson ImmunoResearch Labs Cat# 703-545-155, RRID:AB_2340375)

      Curator: @bandrow

      SciCrunch record: RRID:AB_2340375


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