Hypothesis

8 Matching Annotations

Sep 2025
dx.doi.org dx.doi.org

Rapid CRISPR–Cas9 target-strand nicking can provide phage resistance by reducing DNA abundance

1
1. scibot 27 Sep 2025
  
  in Public
  
  RRID:Addgene_39312
  
  DOI: 10.1093/nar/gkaf900
  
  Resource: RRID:Addgene_39312
  
  Curator: @scibot
  
  SciCrunch record: RRID:Addgene_39312
  
  What is this?
  
  RRID:Addgene_39312
Visit annotations in context

Tags

RRID:Addgene_39312

Annotators

scibot

URL

dx.doi.org/10.1093/nar/gkaf900
Jun 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

A streamlined tandem affinity purification of His-MBP-SpyCas9, without buffer exchange, suitable for in vitro cleavage applications

2
1. scibot 14 Jun 2025
  
  in Public
  
  39,312
  
  DOI: 10.1016/j.mex.2025.103368
  
  Resource: RRID:Addgene_39312
  
  Curator: @olekpark
  
  SciCrunch record: RRID:Addgene_39312
  
  What is this?
  
  RRID:Addgene_39312
2. scibot 14 Jun 2025
  
  in Public
  
  39,312
  
  DOI: 10.1016/j.mex.2025.103368
  
  Resource: RRID:Addgene_39312
  
  Curator: @olekpark
  
  SciCrunch record: RRID:Addgene_39312
  
  What is this?
  
  RRID:Addgene_39312
Visit annotations in context

Tags

RRID:Addgene_39312

Annotators

scibot

URL

pmc.ncbi.nlm.nih.gov/articles/PMC12143660/
Sep 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

CRISPR-Cas9 target-strand nicking provides phage resistance by inhibiting replication

1
1. scibot 25 Sep 2024
  
  in Public
  
  RRID:Addgene_39312
  
  DOI: 10.1101/2024.09.05.611540
  
  Resource: RRID:Addgene_39312
  
  Curator: @scibot
  
  SciCrunch record: RRID:Addgene_39312
  
  What is this?
  
  RRID:Addgene_39312
Visit annotations in context

Tags

RRID:Addgene_39312

Annotators

scibot

URL

ncbi.nlm.nih.gov/pmc/articles/PMC11398490/
Jan 2024
academic.oup.com academic.oup.com

Systematic in vitro specificity profiling reveals nicking defects in natural and engineered CRISPR–Cas9 variants

1
1. scibot 17 Jan 2024
  
  in Public
  
  RRID:Addgene_39312
  
  DOI: 10.1093/nar/gkab163
  
  Resource: RRID:Addgene_39312
  
  Curator: @Naa003
  
  SciCrunch record: RRID:Addgene_39312
  
  What is this?
  
  RRIDCUR:Validated RRID:Addgene_39312
Visit annotations in context

Tags

RRID:Addgene_39312

RRIDCUR:Validated

Annotators

scibot

URL

academic.oup.com/nar/advance-article/doi/10.1093/nar/gkab163/6179346
Dec 2023
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Cas9 is mostly orthogonal to human systems of DNA break sensing and repair

1
1. scibot 01 Dec 2023
  
  in Public
  
  RRID:Addgene_39312
  
  DOI: 10.1371/journal.pone.0294683
  
  Resource: RRID:Addgene_39312
  
  Curator: @scibot
  
  SciCrunch record: RRID:Addgene_39312
  
  What is this?
  
  RRID:Addgene_39312
Visit annotations in context

Tags

RRID:Addgene_39312

Annotators

scibot

URL

ncbi.nlm.nih.gov/pmc/articles/PMC10686484/
Jul 2021
elifesciences.org elifesciences.org

Structural and functional insights into the bona fide catalytic state of Streptococcus pyogenes Cas9 HNH nuclease domain

1
1. scibot 29 Jul 2021
  
  in Public
  
  Addgene: pMJ806
  
  DOI: 10.7554/eLife.46500
  
  Resource: RRID:Addgene_39312
  
  Curator: @Naa003
  
  SciCrunch record: RRID:Addgene_39312
  
  What is this?
  
  RRID:Addgene_39312 RRIDCUR:Missing
Visit annotations in context

Tags

RRID:Addgene_39312

RRIDCUR:Missing

Annotators

scibot

URL

elifesciences.org/articles/46500
Mar 2021
academic.oup.com academic.oup.com

Systematic in vitro specificity profiling reveals nicking defects in natural and engineered CRISPR–Cas9 variants

1
1. scibot 27 Mar 2021
  
  in Public
  
  RRID:Addgene_39312
  
  DOI: 10.1093/nar/gkab163
  
  Resource: RRID:Addgene_39312
  
  Curator: @scibot
  
  SciCrunch record: RRID:Addgene_39312
  
  What is this?
  
  RRID:Addgene_39312
Visit annotations in context

Tags

RRID:Addgene_39312

Annotators

scibot

URL

academic.oup.com/nar/advance-article/doi/10.1093/nar/gkab163/6179346