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Nov 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Enhanced cleavage of genomic CCR5 using CASX2Max

1
1. scibot 24 Nov 2025
  
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  plasmid_61
  
  DOI: 10.1080/15476286.2025.2577449
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12562720/
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PAM-readID is a rapid, simple, and accurate PAM determination method for CRISPR-Cas enzymes in mammalian cells

1
1. scibot 21 Nov 2025
  
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  Addgene_61591
  
  DOI: 10.1038/s42003-025-08984-y
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12627570/
Sep 2025
www.sciencedirect.com www.sciencedirect.com

Ultrabright chemical labeling enables rapid neural connectivity profiling in large tissue samples

1
1. scibot 19 Sep 2025
  
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  plasmid_61591
  
  DOI: 10.1016/j.neuron.2025.08.022
  
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sciencedirect.com/science/article/pii/S0896627325006579
Aug 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

ENHANCED CLEAVAGE OF GENOMIC CCR5 USING CASX2Max

1
1. scibot 11 Aug 2025
  
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  plasmid_61591
  
  DOI: 10.1101/2025.07.08.663680
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12265720/
Jul 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons

1
1. scibot 31 Jul 2025
  
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  RRID:Addgene_61591
  
  DOI: 10.1016/j.xpro.2025.103945
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12305205/
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Focused ultrasound‐mediated APOE4 knockdown in mouse brain

1
1. scibot 20 Jul 2025
  
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  plasmid_61591
  
  DOI: 10.1002/alz.70464
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12263348/
www.biorxiv.org www.biorxiv.org

Engineering a human-based translational activator for targeted protein expression restoration

1
1. scibot 15 Jul 2025
  
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  Addgene_61591
  
  DOI: 10.1101/2025.07.09.663984
  
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biorxiv.org/content/10.1101/2025.07.09.663984v1
Jun 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

CRISPR-Cas Genome Editing in Ex Vivo Human Lungs to Rewire the Translational Path of Genome-Targeting Therapeutics

1
1. scibot 23 Jun 2025
  
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  RRID:Addgene_61591
  
  DOI: 10.1089/hum.2023.223
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11386987/
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CRISPR antiviral inhibits neurotrophic JC polyomavirus in 2D and 3D culture models through dual-gRNA excision by SaCas9

1
1. scibot 14 Jun 2025
  
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  RRID:Addgene_61591
  
  DOI: 10.1016/j.omtn.2025.102556
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12159223/
Apr 2025
physoc.onlinelibrary.wiley.com physoc.onlinelibrary.wiley.com

Neuron–non-neuron electrical coupling networks are involved in chronic stress-induced electrophysiological changes in lateral habenular neurons

1
1. scibot 03 Apr 2025
  
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  plasmid_61591
  
  DOI: 10.1113/JP287286
  
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physoc.onlinelibrary.wiley.com/doi/10.1113/JP287286
Mar 2025
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Single-cut gene therapy in a one-step generated rhesus monkey model of Duchenne muscular dystrophy

1
1. scibot 29 Mar 2025
  
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  RRID:Addgene_61591
  
  DOI: 10.1016/j.xcrm.2025.102037
  
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sciencedirect.com/science/article/pii/S2666379125001107
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Non-invasive detection of allele-specific CRISPR-SaCas9-KKH disruption of TOR1A DYT1 allele in a xenograft mouse model

1
1. scibot 23 Mar 2025
  
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  plasmid_61591
  
  DOI: 10.1016/j.omtn.2025.102466
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11925580/
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Molecularly distinct striatonigral neuron subtypes differentially regulate locomotion

1
1. scibot 22 Mar 2025
  
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  plasmid_61591
  
  DOI: 10.1038/s41467-025-58007-x
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11923167/
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Electroporation Induces Unexpected Alterations in Gene Expression: A Tip for Selection of Optimal Transfection Method

1
1. scibot 11 Mar 2025
  
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  61591
  
  DOI: 10.3390/cimb47020091
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11854308/
Feb 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction

1
1. scibot 16 Feb 2025
  
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  Addgene_61591
  
  DOI: 10.1186/s12864-025-11317-2
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11823040/
Jan 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Rational engineering of minimally immunogenic nucleases for gene therapy

1
1. scibot 27 Jan 2025
  
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  Addgene_61591
  
  DOI: 10.1038/s41467-024-55522-1
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11696374/
Dec 2024
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

All-in-one AAV-mediated Nrl gene inactivation rescues retinal degeneration in Pde6a mice

1
1. scibot 27 Dec 2024
  
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  61591
  
  DOI: 10.1172/jci.insight.178159
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11665581/
www.biorxiv.org www.biorxiv.org

A Versatile Anti-CRISPR Platform for Opto- and Chemogenetic Control of CRISPR-Cas9 and Cas12 across a Wide Range of Orthologs

1
1. scibot 06 Dec 2024
  
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  RRID:Addgene_61591
  
  DOI: 10.1101/2024.11.25.625186
  
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biorxiv.org/content/10.1101/2024.11.25.625186v1
Nov 2024
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Patch and matrix striatonigral neurons differentially regulate locomotion

1
1. scibot 07 Nov 2024
  
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  plasmid_61591
  
  DOI: 10.21203/rs.3.rs-4468830/v1
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11230471/
Aug 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Suppression of HSV-1 infection and viral reactivation by CRISPR-Cas9 gene editing in 2D and 3D culture models

1
1. scibot 26 Aug 2024
  
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  61591
  
  DOI: 10.1016/j.omtn.2024.102282
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11339036/
www.cell.com www.cell.com

Upregulation of utrophin improves the phenotype of Duchenne muscular dystrophy hiPSC-derived CMs

1
1. scibot 26 Aug 2024
  
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  Addgene_6159163
  
  DOI: 10.1016/j.omtn.2024.102247
  
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  RRID:Addgene_61591 RRIDCUR:Unresolved RRIDCUR:Incorrect
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cell.com/molecular-therapy-family/nucleic-acids/abstract/S2162-2531(24)00134-3
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Genome editing in the mouse brain with minimally immunogenic Cas9 RNPs

1
1. scibot 18 Aug 2024
  
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  RRID:Addgene_61591
  
  DOI: 10.1016/j.ymthe.2023.06.019
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10422012/
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Targeted genome editing restores auditory function in adult mice with progressive hearing loss caused by a human microRNA mutation

1
1. scibot 13 Aug 2024
  
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  61591
  
  DOI: 10.1126/scitranslmed.adn0689
  
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ncbi.nlm.nih.gov/pmc/articles/PMC7616320/
Jul 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Long term rescue of Alzheimer’s deficits in vivo by one-time gene-editing of App C-terminus

1
1. scibot 01 Jul 2024
  
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  Addgene_61591
  
  DOI: 10.1101/2024.06.08.598099
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11185791/
Jun 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Conditional knockout of Shank3 in the ventral CA1 by quantitative in vivo genome-editing impairs social memory in mice

1
1. scibot 15 Jun 2024
  
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  plasmid_61591
  
  DOI: 10.1038/s41467-024-48430-x
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11169449/
52.53.155.43 52.53.155.43

Optimization of AAV vectors for transactivator-regulated enhanced gene expression within targeted neuronal populations.

1
1. scibot 06 Jun 2024
  
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  RRID:Addgene_6159
  
  DOI: 10.1016/j.isci.2024.109878
  
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  Curator: @abever99
  
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  RRIDCUR:Unresolved RRID:Addgene_61591
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52.53.155.43/7f9ee4f71b8a925e453a4463d9dde20f671e0e7e/PIIS2589004224011003-html.html
May 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

In vivo genome editing via CRISPR/Cas9-mediated homology-independent targeted integration for Bietti crystalline corneoretinal dystrophy treatment

1
1. scibot 12 May 2024
  
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  61591
  
  DOI: 10.1038/s41467-024-48092-9
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11074121/
Mar 2024
52.53.155.43 52.53.155.43

Efficient and safe therapeutic use of paired Cas9-nickases for primary hyperoxaluria type 1

2
1. scibot 21 Mar 2024
  
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  RRID:Addgene_61591
  
  DOI: 10.1038/s44321-023-00008-8
  
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2. scibot 02 Mar 2024
  
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  RRID:Addgene_61591
  
  DOI: 10.1038/s44321-023-00008-8
  
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52.53.155.43/b4a5d5f166b97df7aae394dba3752d5bbdf7ed06/torella-et-al-2024-efficient-and-safe-therapeutic-use-of-paired-cas9-nickases-for-primary-hyperoxaluria-type-1-html.html
Nov 2023
www.sciencedirect.com www.sciencedirect.com

Unexpected extra exon skipping in the DYSF gene during restoring the reading frame by CRISPR/Cas9

1
1. scibot 17 Nov 2023
  
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  RRID:Addgene_61591
  
  DOI: 10.1016/j.biosystems.2023.105072
  
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sciencedirect.com/science/article/pii/S0303264723002472
Sep 2023
www.mdpi.com www.mdpi.com

Targeted Delivery of Chimeric Antigen Receptor into T Cells via CRISPR-Mediated Homology-Directed Repair with a Dual-AAV6 Transduction System

1
1. scibot 30 Sep 2023
  
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  RRID:Addgene_61591
  
  DOI: 10.3390/cimb45100486
  
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mdpi.com/1467-3045/45/10/486
Aug 2023
www.sciencedirect.com www.sciencedirect.com

Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviors

1
1. scibot 03 Aug 2023
  
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  Addgene_61591
  
  DOI: 10.1016/j.cub.2023.07.013
  
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sciencedirect.com/science/article/pii/S096098222300920X
Jul 2023
onlinelibrary.wiley.com onlinelibrary.wiley.com

Production of adeno-associated viral vector serotype 6 by triple transfection of suspension HEK293 cells at higher cell densities

1
1. scibot 05 Jul 2023
  
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  RRID:Addgene_61591
  
  DOI: 10.1002/biot.202300051
  
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onlinelibrary.wiley.com/doi/10.1002/biot.202300051
May 2023
www.authorea.com www.authorea.com

High-cell-density production of adeno-associated viral vector serotype 6 by triple transfection in suspension HEK293 cell cultures

1
1. scibot 13 May 2023
  
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  RRID:Addgene_61591
  
  DOI: 10.22541/au.167535214.41739207/v1
  
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authorea.com/users/582383/articles/622468-high-cell-density-production-of-adeno-associated-viral-vector-serotype-6-by-triple-transfection-in-suspension-hek293-cell-cultures
Apr 2023
www.biorxiv.org www.biorxiv.org

Genome editing in the mouse brain with minimally immunogenic Cas9 RNPs

1
1. scibot 19 Apr 2023
  
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  RRID:Addgene_61591
  
  DOI: 10.1101/2023.04.13.536294
  
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biorxiv.org/content/10.1101/2023.04.13.536294v1
Mar 2023
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Correction of F8 intron 1 inversion in hemophilia A patient-specific iPSCs by CRISPR/Cas9 mediated gene editing

1
1. scibot 26 Mar 2023
  
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  RRID:Addgene_61591
  
  DOI: 10.3389/fgene.2023.1115831
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10033665/
Dec 2022
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Non-uniform dystrophin re-expression after CRISPR-mediated exon excision in the dystrophin/utrophin double-knockout mouse model of DMD

1
1. scibot 02 Dec 2022
  
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  RRID:Addgene_61591
  
  DOI: 10.1016/j.omtn.2022.10.010
  
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ncbi.nlm.nih.gov/pmc/articles/PMC9664411/
Sep 2022
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Generation of a homozygous P4HA2 knockout human embryonic stem cell line (FDCHDPe012-A) by CRISPR/Cas9 system

1
1. scibot 30 Sep 2022
  
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  Addgene_61591
  
  DOI: 10.1016/j.scr.2022.102930
  
  Resource: RRID:Addgene_61591
  
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sciencedirect.com/science/article/pii/S1873506122002793
Jun 2022
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Deficiency in endocannabinoid synthase DAGLB contributes to early onset Parkinsonism and murine nigral dopaminergic neuron dysfunction

1
1. scibot 22 Jun 2022
  
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  plasmid_61591
  
  DOI: 10.1038/s41467-022-31168-9
  
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ncbi.nlm.nih.gov/pmc/articles/PMC9205912/
Mar 2022
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Regulation of GTPase function by autophosphorylation

1
1. scibot 04 Mar 2022
  
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  AddgeneCat#: 61591
  
  DOI: 10.1016/j.molcel.2022.02.011
  
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sciencedirect.com/science/article/pii/S1097276522001162
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Antiviral Targeting of Varicella Zoster Virus Replication and Neuronal Reactivation Using CRISPR/Cas9 Cleavage of the Duplicated Open Reading Frames 62/71

1
1. scibot 04 Mar 2022
  
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  RRID:Addgene_61591
  
  DOI: 10.3390/v14020378
  
  Resource: RRID:Addgene_61591
  
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ncbi.nlm.nih.gov/pmc/articles/PMC8880005/
Dec 2021
elifesciences.org elifesciences.org

scAAVengr, a transcriptome-based pipeline for quantitative ranking of engineered AAVs with single-cell resolution

2
1. scibot 01 Dec 2021
  
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  RRID:Addgene_61591
  
  DOI: 10.7554/eLife.64175
  
  Resource: RRID:Addgene_61591
  
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  RRID:Addgene_61591
2. scibot 01 Dec 2021
  
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  Plasmid_61591RRID
  
  DOI: 10.7554/eLife.64175
  
  Resource: RRID:Addgene_61591
  
  Curator: @evieth
  
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  RRID:Addgene_61591 RRIDCUR:Unresolved
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elifesciences.org/articles/64175
Oct 2021
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Bombesin-like peptide recruits disinhibitory cortical circuits and enhances fear memories

1
1. scibot 05 Oct 2021
  
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  plasmid_61591
  
  DOI: 10.1016/j.cell.2021.09.013
  
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sciencedirect.com/science/article/pii/S0092867421010606
Sep 2021
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Unlocking loxP to Track Genome Editing In Vivo

1
1. scibot 04 Sep 2021
  
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  RRID:Addgene_61591
  
  DOI: 10.3390/genes12081204
  
  Resource: RRID:Addgene_61591
  
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ncbi.nlm.nih.gov/pmc/articles/PMC8394901/
Aug 2021
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Generation of a homozygous LRPAP1 knockout human embryonic stem cell line (FDCHDPe009-B) by CRISPR/Cas9 system

1
1. scibot 28 Aug 2021
  
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  Addgene_61591
  
  DOI: 10.1016/j.scr.2021.102516
  
  Resource: RRID:Addgene_61591
  
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sciencedirect.com/science/article/pii/S1873506121003639
www.mdpi.com www.mdpi.com

Adeno-Associated Vector-Delivered CRISPR/SaCas9 System Reduces Feline Leukemia Virus Production In Vitro

1
1. scibot 24 Aug 2021
  
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  RRID:Addgene_61591
  
  DOI: 10.3390/v13081636
  
  Resource: RRID:Addgene_61591
  
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mdpi.com/1999-4915/13/8/1636/htm
www.jneurosci.org www.jneurosci.org

Subretinal Human Umbilical Tissue-Derived Cell Transplantation Preserves Retinal Synaptic Connectivity and Attenuates Müller Glial Reactivity

1
1. scibot 01 Aug 2021
  
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  Plasmid_61591
  
  DOI: 10.1523/JNEUROSCI.1532-17.2018
  
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jneurosci.org/content/38/12/2923
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CRISPR-READI: Efficient Generation of Knockin Mice by CRISPR RNP Electroporation and AAV Donor Infection

1
1. scibot 01 Aug 2021
  
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  RRID:Addgene_61591
  
  DOI: 10.1016/j.celrep.2019.05.103
  
  Resource: RRID:Addgene_61591
  
  Curator: @ethanbadger
  
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  RRID:Addgene_61591 RRIDCUR:Validated
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sciencedirect.com/science/article/pii/S2211124719307429
Apr 2021
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Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system

1
1. scibot 09 Apr 2021
  
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  Addgene_61591
  
  DOI: 10.1016/j.scr.2021.102342
  
  Resource: RRID:Addgene_61591
  
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  RRIDCUR:Validated RRID:Addgene_61591
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sciencedirect.com/science/article/pii/S1873506121001884
Sep 2020
www.jneurosci.org www.jneurosci.org

Dopaminergic signaling in the nucleus accumbens modulates stress-coping strategies during inescapable stress

1
1. scibot 26 Sep 2020
  
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  plasmid_61591
  
  DOI: 10.1523/JNEUROSCI.0444-20.2020
  
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  RRIDCUR:Validated RRID:Addgene_61591
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jneurosci.org/content/40/38/7241
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The Raphe Dopamine System Controls the Expression of Incentive Memory

1
1. scibot 23 Sep 2020
  
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  RRID:Addgene_61591
  
  DOI: 10.1016/j.neuron.2020.02.009
  
  Resource: RRID:Addgene_61591
  
  Curator: @ethanbadger
  
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  RRIDCUR:Validated RRID:Addgene_61591
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sciencedirect.com/science/article/pii/S0896627320301082
Jan 2020
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Programmable RNA-Guided RNA Effector Proteins Built from Human Parts

1
1. scibot 13 Jan 2020
  
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  RRID:Addgene_61591
  
  DOI: 10.1016/j.cell.2019.05.049
  
  Resource: RRID:Addgene_61591
  
  Curator: @Naa003
  
  SciCrunch record: RRID:Addgene_61591
  
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  RRID:Addgene_61591 RRIDCUR:Validated
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sciencedirect.com/science/article/pii/S0092867419306208