4 Matching Annotations
- Jun 2019
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Mean Somatotypes (5)
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- May 2019
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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high osmolarity conditions (Gowrishankar, 1989; Csonka, 1989) for β-galactosidase assay
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Assays for determination of β-galactosidase enzyme activity in cultures were performed as described by Miller (1992) after permeabilizing the cells with SDS/chloroform, and the activity values were calculated in Miller units, as defined therein. For determination of proU activity from a proU::lac fusion that contains the proUpromoter cloned upstream of the lacZYA genes (as in plasmid pHYD272), cultures used were grown in LBON or K-medium (low osmolarity medium) since proU is also induced under
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β-Galactosidase assay
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