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    1. developed by Okanagan Specialty Fruits Company in Canada, and the Golden Delicious, Granny Smith, and Fuji varieties have received approval for cultivation, human consumption and use as animal feed in both Canada and the US

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    2. Okanagan Specialty Fruits developed Arctic® Apple events GD743 (Golden Delicious), GS784 (Granny Smith)33 and GS784 (Fuji)35 using their patented method to limit quinone biosynthesis

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    3. Fruit quality is affected by the activity of polyphenoloxidases (PPOs), which oxidize phenolic compounds and cause gradual browning in fleshy fruits such as apple.

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    4. Currently, there are three commercial varieties of Arctic® apple: Arctic® Golden Delicious, Arctic® Granny Smith, and Arctic® Fuji. Commercial harvest of Arctic® Golden Delicious and Arctic® Granny Smith started in 2016, and Arctic® Fuji will be on the market in 202135.

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    5. to maintain the characteristics of the original variety the transgenic events should be derived from mature tissue (such as leaves) rather than embryogenic explants3.

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    6. Genetic engineering allows improved varieties to be developed more quickly, and the vegetative propagation of fruit trees allows the engineered cultivars to achieve coverage of larger areas than crops that depend on sexual reproduction2

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