I really like the idea of the xr glasses collecting data that can then be used to inform the world model for the co-bot, fascinating approach!

I think taking advantage of Opentrons csv parameter abilities to modify scripts is a great approach! I'd be curious to know if you have come across any edge cases when users are modifying tables in excel and how you have approached them? Things like adding spaces, capitalizations, typos etc

I think in part A of this figure the illustration describing the action in between plates during the manual steps is not immediately clear. I think it is supposed to represent a multichannel pipette?

It would be interesting to know what percentage of these scripts you were able to run on the opentrons, I've seen scripts that pass simulation but then immediately throw errors when uploaded to the robot

I'd be interested to see this comparison done with other agentic approaches like Claude Code or Cursor if they were given the same kind of context upfront

Any hypothesis for why this might be? It would also be helpful to include the name and part number for both plate types

I really like this idea for mounting 96 well plates!

Descriptions missing for C and D

Where has this been previously described? A link to a citation here would be helpful

You mention the relative laser intensities, it would be helpful to provide the laser power as well.

It was great to read how you were able achieve this on the OT2. I'm wondering, if cultures can be non isogenic, wouldn't it be more straightforward to transfer transformed cultures to selective liquid media plates instead of dealing with the complexities of calibrating for agar plates? I'd love to hear your thoughts, thanks!

I really like this approach to making structured and reproducible Opentrons protocols without generating entire new scripts each time. I'm curious how LLMs might fit in upstream of this, could you describe your protocol in plain language, have the LLM generate the csv files, then run protocol generation script? Could the LLM just generate the protocol at this point?

Really fascinating method for culturing, screening and imaging all on one platform. Using pillars to 'cage' the c. elegens while allowing liquid to pass through is a great idea. I'd be curious if you have thought of any ways this design could be adapted to sort c. elegens into collection chambers based on their phenotype?

I really like this implementation of tilting the plate with a custom holder instead of a variable tilt mechanism, will consider using something like this for our opentrons!

It would be helpful to know what the power output of the light is, what wavelength it emits, and how far the light source is from the petri dish.

Instead of adding the second beam to counteract the force of the light sheet, is it possible to add a 3D sheath flow that pushes cells toward the bottom of the channel? This paper explains this concept https://doi.org/10.1063/5.0033291

iCLIP looks like a really promising tech for making 3D printed microfluidics, it was great to learn more about it through your paper. I wonder how long you could have resin 3D printed structures like these in your skin without negative side effects? I could imagine devices like these being built into wearables that can inject drugs continuously over longer periods of time

I'm really looking forward to seeing how this goes and would be interested in building one myself!

I think its great to be able to use 3D printer resin and still get high quality structures. I'd be curious to see a comparison of devices made with SU-8 vs 3D printer resin using this maskless approach, are there still benefits to SU-8 that make investing in the proper safety equipment worth it? It would also be interesting to see if there is still any benefit to using silicon over glass

Might be worth trying a darker opaque resin, this paper showed a reduction in surface roughness when using phrozen High Temp 3D Printer Resin [TR250LV]. https://doi.org/10.1038/s41378-023-00607-y

I'm curious at what orientation you printed these molds? This paper showed a reduction in surface roughness when tilting multiple axis of the model. https://doi.org/10.1038/s41378-023-00607-y . Not sure if this same technique will translate between DLP and SLA though

I think it would be helpful to label Figure 3E with the molding techniques to get a better sense of what is actually happening to the PDMS during the curing step

I think the description for A and B in Figure 2 were swapped

should be 'silanized' with an 'i'

It might be helpful to walk through what dimensions went into this Reynolds number estimation.

What were the conditions for the PDMS curing?

It's difficult to understand what exactly the distance vs gray scale chart is referring to underneath each of the USAF targets. A short explanation here would be helpful.

Thank you for including this information, I have tried similar to make similar microfluidic channels of different dimensions, but did not consider the differences that light source wavelength would have on different resin types.

It was great reading about an open-sourced imaging platform being used as a low cost method of disease detection. I'm curious how this system works with other cameras and what trade-offs there are between this camera and others you may have bought. I've been using FLIR cameras for open-source affordable imaging projects, but I am not sure that is the best option.

Really great reading about this device! Has anyone tried using it for nucleus extractions of algae? We have used a modified version of this protocol where we cryo-grind Chlamydomonas with a mortar and pestle, but found it to be laborious and inconsistent. I'd imagine you'd need to make significant modifications to cool the system, though that might be beneficial due to the heat that is generated from the motor

I really like this approach to plant imaging, I'm curious what you think about a system like this that allows the camera to move between positions to take time lapse images of even more samples?

I really like this approach to plant imaging, I'm curious what you think about a system like this that allows the camera to move between positions to take time lapse images of even more samples?

I've really enjoyed reading this paper, learned a lot about optigenetics and ocular implanted devices. I'm curious how you plan to validate the effectiveness of these devices in animals and what kinds of assays would make the most sense.

I really enjoyed reading about this project and am curious about implementing something like this for our lab! It would be great to see a video of it in action. It would also be great to know how quickly COPICK can pick colonies from a plate vs a person in the lab?

Has this changed in the last year? Would love to know where advancements are being made

I'm curious if there are any drawbacks to training the model using a different camera than the one that is implemented on the OT-2?