5 Matching Annotations
  1. Aug 2022
    1. Allostatic overload resulting from chronic stress inanimal models causes atrophy of neurons in the hip-pocampus and prefrontal cortex, brain regions involved inmemory, selective attention, and executive function, andcauses hypertrophy of neurons in the amygdala, a brainregion involved in fear and anxiety as well as aggression(213) (see sect.IV). Thus the ability to learn and rememberand make decisions may be compromised by chronicstress and may be accompanied by increased levels ofanxiety and aggression.


  2. Oct 2021
  3. Aug 2021
    1. Skin Extract Preparation

      Alarm substance was collected by making shallow lesions on the skin (7 to 10 on each side) using a sharp razor (Sharpoint, 22.5o stab), such that drawing of blood was minimal. Fish were then immersed in 2 ml of 20 mM Tris-Cl (pH 8.0) for 20-30 seconds. The process was repeated for 7 fish and extract collected into the same volume. The 2 ml crude extract was then centrifuged at 13.2k rpm and filtered. All steps were performed at 4 ̊C. 1 unit of extract was defined as the minimal amount of skin extract that must be delivered into the test tank to elicit freezing. A typical extraction yielded 20 units.

      Sloughing was performed by mechanical shaking, either by inverting a 2 ml eppendorf tube with a euthanized fish without lesions or cuts in 2 ml of 20 mM Tris-Cl (pH 8.0), rapidly many times or on an orbital shaker for 1’.

      For estimation of chondroitin concentration extraction was performed in 100 mM CH3COONH4 as described.

  4. Jul 2021
    1. 此研究的研究对象是健康足月胎儿,最终可用样本量为19,观察他们对连续24次震动刺激的反应,当对连续的4个刺激不产生反应时视为习惯化。每个研究对象进行3次实验,第1次实验结束的10分钟后和24小时后重复测试,以观察短期记忆和长期记忆能力。

  5. Jun 2021