Purpose Dealing with variants of unknown significance (VUS) is an important issue in the clinical application of NGS-basedcancer gene panel tests. We detected a novel ERBB2 extracellular domain VUS, c.1157A > G p.(E401G), in a cancer genepanel test. Since the mechani

H3 K27M mutations were first describedin pediatric diffuse pontine gliomas (DIPGs)[1], but soon thereafter were found in mid-line gliomas in adults by our group [2] andSolomon et al. [