The poxviruses are unique compared to other DNA viruses in that they replicate their genome in the cytoplasm
Both LCMV (RNA) and Smallpox Virus (DNA) replicate their genome in the cytoplasm.
The poxviruses are unique compared to other DNA viruses in that they replicate their genome in the cytoplasm
Both LCMV (RNA) and Smallpox Virus (DNA) replicate their genome in the cytoplasm.
There has been no evidence of person-to-person transmission
LCMV is transmitted from mouse to human and Smallpox Virus is transmitted from human-human.
Proteus and Enterobacter are only present in a minority of humans [64].
very rare
The patient was treated topically with moxifloxacin (0.5%) and tobramycin (15 mg/ml) drops every hour and cyclopentolate 2% three times daily in the right eye. N-acetylcysteine eye drops were administered four times per day.
treatment for the case
Samples from conjunctival swabbing and corneal scraping, the contact lens, the cleaning solution, and the case were all submitted for microbiological analysis.
what we should do to diagnose the case
enterobac-teria (such asSerratia spp.andKlebsiella spp.) that can survive in contact lens fluid and onplastic surfaces, which explains their increased numbers incontact-lens-induced corneal infectio
growth in this certain environment by enterobacteria
Mechanisms of Beta Lactam Resistance in Enterobacter
beta-lactam (ampicillin) resistance mechanism
grow optimally at 37 to 42 °C.[5][6][7][8] When exposed to atmospheric oxygen, C. jejuni is able to change into a coccal form
growing conditions
oxidase-positive
oxidase test
C. jejuni is also commonly found in animal feces.
where it's found
14 cases are diagnosed each year for each 100,000 persons in the population.
prevalence rate
Combination therapy with antipseudomonal antibiotics is used to ensure treatment of resistant strains and to prevent selection of resistant mutants.
reason why we need to use combination therapy
multidrug-resistant strains were isolated more frequently from ICU and nursing home patients.
interesting to note
It uses pili with the aid of protease enzymes in attachment to epithelial cells, such as in the respiratory tract. The bacterium has capsules or slime layers that protect it from antibodies, lymphocytes, and phagocytes. In order to invade tissues, it produces extracellular enzymes such as elastase and alkaline protease. There are also three soluble proteins involved in invasion, which are cytotoxin (mw 25 kDa) and two hemolysins. It produces the extracellular toxins Exoenzyme S and Exotoxin A and also LPS, which is characteristic of Gram-negative bacteria. All of these characteristics lead to a wide variety of diseases.
virulence factors
The bacterium is very low-maintenance when it comes nutrition. It does not require organic growth factors, and can use over seventy-five organic compounds for growth. In a lab setting, acetate and ammonium sulfate in a medium will satisfy it. The optimum growth temperature is 37 degrees C, but it can grow in temperatures up to 42 degrees C. Not only is it tolerant to temperature, but also to salts and dyes, weak antiseptics, and antibiotics.
growth conditions
It is not an active fermenter of carbohydrates and produces acid, but no gas, in glucose and is lactose-negative. The oxidase and catalase test for P.aeruginosa are positive.
catalase and oxidase test
so the media needed for growth and isolation of the organism contain hemoglobin, NAD, yeast extract and other supplements.
More growth requirements
he recommended procedure for isolating Neisseria gonorrhoeae involves the inoculation of a specimen directly onto a nutritive growth medium that is at room temperature and immediate incubation at 35-37ºC in an atmosphere of 3 – 10% added CO2
Some aspects of growing conditions
The organism is usually found interacellulary in polymorphonuclear leukocytes, or a specific category of white blood cells with varying shapes of nuclei, of the gonorrhea pustular exudates [8] with humans as its only natural host
Answer to question 4: Where is the organism found normally?
Beta-hemolytic colonies of Streptococcus pyogenes on sheep blood agar. Cultivation 24 hours, aerobic atmosphere, 37°C.
Colony morphology
Highly contagious and potentially fatal, tularemia usually can be treated effectively with specific antibiotics if diagnosed early.
Must run blood tests and culture as quickly as possible for sake of patient's health
Blood tests and cultures can help confirm the diagnosis
because of difficulty to diagnose, you must culture and run blood tests for the patient to be confirm that the diagnosis is tularemia
Tick and deer fly bites Skin contact with infected animals Ingestion of contaminated water Inhalation of contaminated aerosols or agricultural dusts Laboratory exposure
examples of mode of transmission
a total of 856 cases of human infections with H5N1 viruses worldwide have been documented, and there have been 452 deaths
Nearly a 53% fatality rate from H5N1!