The fluorescence signal atCT37±7 corre-sponds to the no-template-control andrepresents bacterial DNA contamination inthe commercially supplied reagents.
2,422 Matching Annotations
- Mar 2019
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www.microbiologyresearch.org www.microbiologyresearch.org
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www.nature.com www.nature.com
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insulation of minimal promoters was demonstrated
(citation 35) excerpts σ70-dependent promoter cores are not modular in function and, therefore, are not a good choice for use in the bottom-up design of promoters
promoter cores of PECF11 and PT7 are comparatively insensitive to the sequence context imposed by operators
(fig 1) Natural promoters from bacteria and phages were genetically refined to identify insulated promoter cores (minimal promoters for which transcriptional activity is independent of sequence context). b Natural single operators were permuted and refined to create synthetic operators with diverse transcriptional regulation properties, designed to be free of spontaneous promoter activity. c and d Insulated promoter cores c and synthetic operators d were experimentally characterized and parameterized as transcriptional activation- and repression-dependent parameter databases, respectively, via biophysical modeling. e Genetic circuits including both basic genetic gates and complex transcriptional networks can be precisely designed using insulated transcriptional elements
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www.nature.com www.nature.com
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each with 80% power (chance) of achieving P < 0.05
What is the meaning of this sentence?
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science.sciencemag.org science.sciencemag.org
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real-time information
How does memory provide real-time information? It's an integral of all past information
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- Feb 2019
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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Several aspects influence PCR yield and specificity: reagents concentration, primer and amplicon length, template and primer secondary structure, or G+C content
There are other overlooked factors here - presence of inhibitors, Tm of reaction compared to primer thermodynamics
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- Dec 2018
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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suggesting recent horizontal acquisitions
Has been found in 2 species that have been sequenced.
It is possible that it exists in other unsequenced species
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bmcmicrobiol.biomedcentral.com bmcmicrobiol.biomedcentral.com
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travelling
Doesn't it depend on where the child is travelling??
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link.springer.com link.springer.com
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negative controls containing AF1000 cells devoid of plasmid yielded Ct numbers higher than 26 and were automatically censored by the LightCyclerTM software.
What's the explanation for this!?
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www-sciencedirect-com.ezproxy.rice.edu www-sciencedirect-com.ezproxy.rice.edu
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Fig. 4
Graph is extremely unclear. Bad usage of point shapes
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hub.jhu.edu hub.jhu.edu
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a shrinking number of people have personal memories
How is it that the virus still persists!? It is quite impressive
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peer-reviewed research
The quality of peer-review is definitely at fault here. It could also undermine public faith in the peer-review process and the 'scientific community'
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the difference in how scientists and the general public perceive risk
Information asymmetry
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a loss of personal freedom
Personal freedom is quite a misconstrued concept. People miss the fact that vaccinations are not as personal as they seem to be. Vaccinations have a significant effect on the population through herd immunity.
Can someone clarify in general, what the hell is personal freedom?
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www-nature-com.ezproxy.rice.edu www-nature-com.ezproxy.rice.edu
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observed reduction was likely an underestimate due to the bimodal circuit activation in each population
Oh, it is not easy to grow just OFF cells!
- Can we prevent them from turning ON for this measurement to be more precise?
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observation of hysteresis
General definition - Hysteresis is the dependence of the state of a system on its history
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www-sciencedirect-com.ezproxy.rice.edu www-sciencedirect-com.ezproxy.rice.edu
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There are some techniques
Traditional analytical methods of arsenic measurement
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- Nov 2018
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www.makeuseof.com www.makeuseof.com
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Partial charges and discharges that combine to 100% are counted as a single full cycle
Really? I don't think anyone has tested partial charges and discharges. I would like to see a reference for this point!
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research says
That is pretty weak evidence for such a strong statement
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www.nature.com www.nature.com
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low rates of indels (typically no more than 0.1%
0.1% is still a lot from a theraputic standpoint!
Tags
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www.nature.com www.nature.com
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push the technical limits of this information storage system
I don't see anything new in this, technology wise. Cas1-2 will integrate any sequence, whether it codes for pixels or not
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- Oct 2018
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www.politico.com www.politico.com
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But the bully-pulpit talk didn’t do much
That's funny! I like the background of the legacy of George Bush!
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biotium.com biotium.com
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containing ROX reference dye
What's the concentration?
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