8 Matching Annotations
  1. Apr 2019
    1. The tombo filter raw_signal_matching command filters out reads with poor matching between raw observed signal and expected signal levels from the canonical base model. Specify a new threshold to apply with the --signal-matching-score option. These scores are the mean half z-score (absolute value of z-score) taken over all bases of a read.

      These scores are the mean half z-score (absolute value of z-score) taken over all bases of a read.

    1. per read stats #31

    2. what is the stat in myfile.tombo.per_read_stats
    3. The stat field of the per-read statistics file is either a hypothesis test p-value for the sample comparison or de novo testing methods or a log likelihood ratio for the alternative model comparison method.

      the stats in my .per_read_stats should be a de novo testing methods.

  2. Mar 2019
    1. Welcome to Tombo’s documentation!

      在这里可以找到tombo的具体使用方式和文件

    1. Using non-standard basecalling models (e.g. Flip-flop)

      QG说使用flip-flop模型,如果某一个信号无法被转换为ATCG等标准等碱基序列,那么他会被标示出异常。

    1. error BadZipfile: File is not a zip file

      在安装albacore的时候,使用pip install命令安装.whl文件的时候,总是会出现“ BadZipfile:File is not a zip file”的错误,通过使用参数--no-cache-dir可以解决这个问题。

    1. Nanopore data and downstream anlysis

      In this post, only explain pass, fail and skip in nanopore sequencing.