Total haemocyte count (THC)

Haemolymph collection

Immunomodulatory

Lactate dehydrogenase

Asparate (AAT) and Alanine aminotransferase (ALT)

Gut enzyme profile

Oral toxicity bioassay

Microinjection bioassay

VS preparation

Insect collection

Statistical analysi

Quantification of VS for protein

Venomous saliva utilization

Prey type

Starvation and collection method

Venomous saliva optimization

Insects Collection

Gross morphology and histology of salivary gland

Morphometry of head and stylet

Head and stylet preparation

Insect collection and maintenance

Statistical analysis

Micronuclei test

SDS -PAGE for serum protein analysis

Estimation of serum proteins (SDS –PAGE) and Micronuclei in fish, Labeo rohita

Estimation of bioaccumulation of trace metals inmuscle and gill tissue of fish, Labeo rohita

Lactic dehydrogenase

Malic dehydrogenase

Succinic dehydrogenase

Estimation of dehydrogenases activities (SDH, MDHand LDH) in muscle and gill tissue of fish, Labeo rohita

Alkaline Phosphatase

Acid Phosphatase

Estimation of Phosphatases activities (Acid and Alkaline Phosphatase) in muscle and gill tissue of fish, Labeo rohita

Cholesterol content

Glycogen content

Protein content

Estimation of biomolecules (Protein, glycogen and cholesterol) inmuscle and gill tissue of fish, Labeo rohita

Fish sampled from selected water bodies for further analysis

Characteristic features ofLabeo rohita

Animal model for the study-Labeo rohita

Analysis of Physico-chemical parameters of the water sampled

Survey of lakes to check their status of pollution

Experimental Set up

Organic carbon

Soil pH and electrical conductivity (EC)

Mechanical composition

Methods of soil analyses

Soil sampling

Experiment 2: Assessing the impacts of elevated temperature and N levels on yield and nutrient uptake in rice

Experiment 1: Assessing the impacts of elevated CO2and N levels on yield and nutrient uptake in rice

Experimental Design and Treatments

Temperature Gradient Tunnels (TGT)

Free Air Carbon dioxide Enrichment (FACE)Facility

Site

Statistical analysis

Kernel hardness

SDS-sedimentation test

Estimation of total N% of wheat grainsand straw

End use Quality parameters

Chlorophyllcontent

Root length (cm) and Root weight (mg)

Coleoptile length(cm)

Stomata / cm2

Leaf area index (LAI)

Physiological parameters

Laboratory observations

Normalized difference vegetation index (NDVI)

Spike length (cm)

Last node to spike length(cm)

Peduncle length(cm)

HarvestIndex

Grain yield per plot (g)

Biological Yield(g)

1000 Kernel weight(mg)

Number of grains per spike

Number of productive tillers per meter

Plant height (cm)

Days to physiological maturity

Days to heading

Field observations

Concentration of ideal carbon source

Carbon sources

NaCl concentration

Temperature

pH

Static and shaken condition

Inoculum concentration

Optimization

Identification of the strains

Screening for proteolytic activity

STUDY AREA

Statistical Analysis

CFSE proliferation assay

CD107a Degranulation Assay

Flow cytometric analysis of cytokine production (IFN-γ)by intracellular staining

Flow cytometric analysis of intracellular CD3ζ chain expression

Cell surface and Intracellular staining and Flow Cytometric Analysis

Freezingor storageof cells

Cell Counting

Separation of Peripheral Blood Mononuclear Cells(PBMCs)from whole blood

Virological assessment: Quantitative RT-PCR detection of HBV DNA

Serological and Virological Assessment

HBV vaccination of the newborn

Sample Collection

Healthy Controls

Statistical analysis

NiSO4. 6H2O stock soloution

Nonidet-P40 (NP40) buffer for Ni-NTA pull down

Low-pH antibody elution buffer

Cytoskeleton extraction buffer (CSE)

Semi dry transfer buffer

GST elution buffer

Ni-NTA elution buffer

Lysis buffer for Ni-NTA and glutathione purification

1X TBST

1X TBS

1X PBS

Composition of buffers and solutions

GST-pull down

Surface plasmon resonance (SPR) measurements

Expression and purification of recombinant proteins

Immunofluorescence studies in SW80 cells for β-catenin under nickel induced condition

Luciferase assay in SW480 cells under nickel induced condition

Ni-NTA pull down

Stripping of Ni-NTA agarose beads

Nuclear and cytoplasmic fractionation

Large scale purification of BPN in CHO cells from pSecTag2 vector

Luciferase reporter assay

Immunoprecipitation and western Blotting

Immunofluorescence

Generation of polyclonal antibodies against GFP, APC and β-catenin

Cell Cycle Analysis

Annexin-V FITC/ PI staining

Analysis of ROS

Cell Viability and IC50determination byMTT assay

Transfection of short interfering RNA (siRNA) and plasmid

Cell culture and drug treatment

Growth of Bacteria

Tissue Culture Medium

Cell Lines

Mice

Bacteria

Procedure

Extraction of Tannin

Reagent

Tannin

Standard

Estimation

Extraction

Reagent

Free amino acid

Calculation

Procedure

Reagent

Proline

Extraction and determination of sugar

Standard curve of sugar

Reagents

Estimation of total sugar

Extraction and determination of protein

Standard curve of protein

Reagents

Estimation of protein

Extraction and determination of ascorbic acid

Standard curve of ascorbic acid

Reagents

Estimation of ascorbic acid

Biochemical

Fresh and Dry weight

Root and Shoot length

Morphological Studies

LC 50 value

Dilution Formula

Cd (NO3)2

ZnSO4

Preparation of ppm solution

Seeds and germination

Immunohistochemistry (IHC)

Assay procedure

ELISA

Dot blot assay

Immunodetection

Immunoblotting

Poly acrylamide gel electrophoresis (PAGE

Extraction of total protein from mammalian cells and quantification

RNA extraction and cDNA synthesis

Flow cytometry

Transwell experiment

Animal injection

LV concentration by ultracentrifugation

Preparation of Lentiviral Vectors

Routine maintenance of cell lines

Mammalian cell culture

shRNA designing

Long PCR

Polymerase chain reaction (PCR)

Gene cloning

DNA fragment isolation from agarose gels

Agarose gel electrophoresis

Large scale preparation of plasmid DNA