Jepa Chandra Mohan, P J

A study on the hydrobiology and biodiversity of Ichthyofauna of Pechiparai Dam

Bharathidasan University

Rameshkumar, A

Profiling of phenolic compound through UPLC-MS/MS and study on pharmaceutical properties of merremia emarginata ( Burm. F.)

Sucrosc, 'frizma base, PMSI:, EIYSA, Tween-40TM IPolyoxycthylene (20) sorbitol ~nonopalmitatej. Tween-20TM, Glycerol, a-n~ercaptoethanol (2-ME), 1)imethyl sulphoxide (DMSO), TEMED. Ammoniu~n persulphate (APS), sodium dodecyl sulfate (SIX). Brilliant Blue G, Coomassie Blue, OPD, N-cthylmaleimide (NEM). Bovine Serum Albumin (USA), azetidinc-2-carboxilic acid (Azt). I,-canavanine (L-can). phosphatidylclioline, pl~osphatidylserine, phosphatidylethanolamine. cycloheximidr, 1,-amino acids, 1)-amino acids, valinomycin, CCCP, PercollTM, I,-proline agarose, L-argir~ine agarose, n-octyl-p-D- glucopyranoside, dansylated amino acids and egg-phoshatidylclioli~le (Egg-PC) were purchased from Sigma Chemicals Co. (St. Louis, USA

Jamia Milia Islamia University

Saxena, Sanjeev

Transport and transporter of L proline of candida albicans

Integral University

Bharathiar University

Anti-inflammatory activity

Acute toxicity studies

Drugs and chemicals

Oral administration of doses

Animal handling and care

Animals

Preparation of distilled extracts

Harvest of germinated seed

Germination of N.sativaseeds

Collection of N.sativaseeds

REFERENCES

Conclusion

ROS ASSAY

In vitro cytotoxic activity of methanolic extract of N. sativafrom different germination phasesduringSRB assay

n vitro cytotoxic activity of methanolic extract of N. sativafrom different germination phasesduringMTT assay

Statisticalanalysis

ROS assay

Cytotoxicity assay by Sulphorhodamine B (SRB) me

Cytotoxicity assay by MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyltetrazolium Bromide)method

Cytotoxicityscreenin

Cell culture

Drugs and chemicals

Preparation of distilled extracts

Harvest of germinated seeds

Germination of N. sativaseeds

Collection of N. sativaseeds

TLC study of alkaloids

TLC study of phenols

TLC study of alkaloids

Activation of TLC plate

Preparation of TLC plate

Study of Phytochemicals by Thin Layer Chromatography (Wagner, and Bladt, 1996)

Test for cardiac glycosides

Test for Terpenoids

Tests for Flavonoids

Tests for Phenolic compounds

Tests for Saponins

Tests for Tannins

Tests for Alkaloids

Test for Sterols

Qualitative study of phytochemicals of N. sativadurin

Inoculum preparation

Clinical bacterial strains used for the study

Preparation of distilled extracts

Harvest of germinated seeds

Germination of N.sativaseeds

Collection of N.sativaseeds

Testing of bioformulations

Preparation of bioformulations and determination of

Validation of bioformulations under laboratory conditions (in vitro

Sequence analysis

DNA sequencing of the 18S rDNA fragment

Purification of PCR product

Analysis of internal transcribed spacer region

RAPDand SSRscoring and data analysis

PCR amplification

Running of gel and visualization of DNA

Determination of the yield

Agarose gel electrophoresis

Qualitative and quantitative estimation of DNA

Determination of the yield

Procedure for DNA isolation

Reagents required for fungal DNA isolationand p

DNA isolation of Trichodermaisolate

Genetic variability analysis through RAPD and SSR

Photography, evaluation and documentation

Procedurefor SDS-PAGE

Materialsrequired for SDS-PAGE

Protein profiling of bioagent through SDS-PAGE

Biochemical analysis (Protein estimation)

Protein estimation through Kjeldahl method

Effect of temperature on xylanase activity

Effect of pH on xylanase activity

Effect of various carbon sourceson xylanase activity

Assayof xylanase activity

Dinitrosalicylate reagent (DNS)(per liter)

Citrate phosphate buffer

Reagents

Xylanase activity

Harvesting of cultures

Enzyme production (EP) medium

Inoculum preparation

Sporulation medium used for Trichodermasp

Maintenance of Trichodermasp. culture

Sterilization

Materials for xylanase induction

Induction of xylanase from Trichodermasp

Evaluation of bioagents against the pathogen

Laboratory screening of antagonists against the test pa

Effect of pHon growth and sporulation of the bioagent

Effect of temperature on growth of bioagent

Effect of different media on growth of bioagentTrichoderma

Cultural characteristicsof bioagent Trichoderma

Mycelial characteristicsof pathogen

Identificationof bioagent

Isolation and purification of Trichoderma sp.

Isolation and purification of pathogen, Fusarium udum

Isolation, purification and morphological characterization of pathogen and bioagent

Symptomatology

Collection of the diseased materia

Sterilization of laminar air flow

Sterilization of media and distilled water

Sterilization of glasswares

Sterilization procedure

Source of chemicals

Experimental site

Department of Marine Biology, Microbiolgy and Bi

Ally C. Antony

Prevalence Risk Assessment and Survival Kinetics of Indicator and Pathogenic Bacteria i

Relative distribution of virulence genes among V. parahaemolyticusfrom Cochin estuary, shrimp farm and sea food

Prevalence of type III secretion system genes amongV. parahaemolyticusfrom Cochin estuary, shrimp farm and seafood

Prevalence of tdhand trhgenes among V. parahaemolyticusfrom Cochin estuary, shrimp farm and seafood

Relative prevalence of extracellular virulence factors among Vibriofrom Cochin estuary, shrimp farm and seafood

Prevalence of extracellular virulence factors in Vibriofrom seafood

Prevalence of extracellular virulence factors in Vibriofrom shrimp farm

Prevalence of extracellular virulence factors in Vibriofrom Cochin estuary

Gel documentation and image analysis

Screening for virulence genes in V. parahaemolyticusfrom Cochin estuary, shrimp farm and seafood

Statistical analysis

Detection of hemolytic activity

Production of caseinase

Production of phosphatase

Bacterial strains used

DNAisolation

Detection of virulence genes tdhand trhby multiplex

Detection of type III secretion system genes

Production of chitinase

Production of DNas

Production of gelatinase

Production of Lipase

Production of amylase

Bacterial strains used

Screening of Vibriostrains for extracellular enzymes

Plasmid curing of Vibriofrom Cochin estuary,shrimp farms and seafood

Plasmid profiles among Vibriofrom Cochin estuary, shrimp farms and seafood

Distribution of antibiotic resistance genes in Vibriofrom Cochin estuary, shrimp farm and seafood

MAR indexingand antibiotic resistance pattern amon

Relative antibiotic resistance amongVibrioisolated from Cochin estuary, shrimp farm and seafood

Antibiotic resistance among Vibriofrom seafood

Antibiotic resistance amongVibriofrom shrimp farm

Antibiotic resistance amongVibriofrom Cochin estu

Antibiotic resistance amongVibrio

Plasmid curing experiment

Plasmid profiling of the drug resistant strains

Gel documentation and image analysis

Detection ofblaNDM-1gene

Detection of blaCTX-Mgene

Detection of blaTEMgene

DNA isolation

Detection of beta-lactam antibiotic resistancegenes

MAR indexing

Antibiotic sensitivity tes

Genbank accession numbers

Seasonal variation in the diversity and distribution of Vibrioin Cochin estuary

Relative diversity and distribution of Vibrioin the water and sediment of Cochin estuary

Species level identification and distribution of Vibriospecies in Cochin estuary

Environmental parameters

Statistical analysis

Gel documentation and image analysis

Detection of toxR gene

Detection of tlhgene

Extraction of genomic DNA

Detection of V. parahaemolyticusspecies-specific gene

Isolation of V. parahaemolyticuson HiCrome Vibrio

Isolation and identification of V. parahaemolyticus

PCR amplification of 16S rRNA gene

DNA isolatio

Molecular confirmation of Vibrioby 16S rRNA gene se

Resistance to ampicillin

Citrate utilisation tes

Nitrate reduction test

Production of β-galactosidase (ortho-Nitrophenyl β-D-galactopyranoside (ONPG)) test

Urease production

Gelatinase production

Growth at different temperatures

Salt tolerance tes

Voges Proskauer (acetoin production) test

Indole production

Carbon source utilisation tes

Carbohydrate fermentation test

Amino acids utilisation test (Decarboxylase/dihydrol

Species level identification

Oxidative-Fermentative test

Oxidase test

Gram staining

Presumptive identification

Isolation of Vibriospecies from water and sediment of Cochin estuary

Sample collection

Analysis of hydrographical parameters