2 Matching Annotations
- Jan 2024
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onlinelibrary.wiley.com onlinelibrary.wiley.com
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generally derives from variations in filtering out spurious and low-abundant sequences (e.g. Edgar, 2017; Prodan et al., 2020).
DADA2 like ASV vs OTU?
Applying different workflows on the same data will always demonstrate a certain level of variation among pipelines. These variations are usually most obvious in terms of the reported number of features.
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- Dec 2021
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fiererlab.org fiererlab.org
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If your data is high quality, you want improved taxonomic resolution, and you are not concerned about the intra-genomic heterogeneity in the targeted marker genes, an ESV-based approach could be advantageous. Otherwise, a more standard OTU-based approach might be your best bet.
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