4 Matching Annotations
- May 2024
- Nov 2020
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www.bio-rad.com www.bio-rad.com
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To detect foreign DNA in 5 ml of lake water, 15 ml of lake water must be screened.
Why is this multiplied by 3? Related to statistical error of subsampling - rule of three)
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- Sep 2020
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www.nature.com www.nature.com
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LOD was defined as <x>bi + ksbi, where <x>bi equals the mean of the no-template controls, sbi is s.d. of no-template controls and k = 2.479 (99% confidence interval)
ddPCR
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- Jul 2020
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www-ncbi-nlm-nih-gov.ezproxy.rice.edu www-ncbi-nlm-nih-gov.ezproxy.rice.edu
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LoD = LoB + 1.645(SD low concentration sample)
LoD is the lowest analyte concentration likely to be reliably distinguished from the LoB and at which detection is feasible. LoD is determined by utilising both the measured LoB and test replicates of a sample known to contain a low concentration of analyte.
LoB is the highest apparent analyte concentration expected to be found when replicates of a blank sample containing no analyte are tested.
LoB = meanblank + 1.645(SDblank)
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