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  1. Feb 2024
  2. www-sciencedirect-com.ezproxy.rice.edu www-sciencedirect-com.ezproxy.rice.edu
    Optimization of single plate-serial dilution spotting (SP-SDS) with sample anchoring as an assured method for bacterial and yeast cfu enumeration and single colony isolation from diverse samples
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    1. pbk1 12 Feb 2024
      Decimal serial dilutions (100–1000 μl) of 101–106 or 107 were prepared from the 100 stock in 1.5 ml tubes with 4–5 repeated flushing and changing of tips

      changing tips seems important?

      CFU protocol serial dilution
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    www-sciencedirect-com.ezproxy.rice.edu/science/article/pii/S2215017X15000454
  3. static-content-springer-com.ezproxy.rice.edu static-content-springer-com.ezproxy.rice.edu
    42003_2020_1127_MOESM1_ESM.pdf
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    1. pbk1 12 Feb 2024
      Pipet 100 μL of Starting Culture into Dilution 1. Discard tip. Do NOT pipette up and down. Vortex tube for 5-10 secs

      serial dilution method. Why should you not pipette up and down? Cells killed due to pipetting stress?

      serial dilution CFU protocol
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    static-content-springer-com.ezproxy.rice.edu/esm/art:10.1038/s42003-020-01127-5/MediaObjects/42003_2020_1127_MOESM1_ESM.pdf