Do not exceed 5,000 copies of target/μl of the final ddPCR reaction mix
1e5 targets per 20 ul reaction well. Sounds a little high no for 20,000 droplets, I would estimate a total of 2,000 copies to keep the avg 0.1 copies per droplet?
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- May 2024
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www.bio-rad.com www.bio-rad.com
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- Nov 2020
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www.bio-rad.com www.bio-rad.com
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average of 5 copies/droplet (the upper end of the recommended loading range), we expect to see about 134 empty droplets in a total of 20,000 droplets
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