102548
DOI: 10.1111/cns.70026
Resource: RRID:Addgene_102548
Curator: @olekpark
SciCrunch record: RRID:Addgene_102548
102548
DOI: 10.1111/cns.70026
Resource: RRID:Addgene_102548
Curator: @olekpark
SciCrunch record: RRID:Addgene_102548
rosophila Bloomington Stock Center
DOI: 10.1111/cns.14527
Resource: Bloomington Drosophila Stock Center (RRID:SCR_006457)
Curator: @maulamb
SciCrunch record: RRID:SCR_006457
Bloomington stock, #5905
DOI: 10.1111/cns.14527
Resource: (BDSC Cat# 5905,RRID:BDSC_5905)
Curator: @maulamb
SciCrunch record: RRID:BDSC_5905
Tissues were then frozen in optimum cutting temperature embedding medium and cryosectioned for direct GFP visualization (brain was sectioned at 30 μm, and other tissues at 8 μm). Images were acquired with a Nikon Eclipse Ti-E fluorescence microscope.
I was initially critical of the sample size they described earlier but reading through this specific protocol makes it seem extremely tedious to collect data. Has anyone here performed these types of studies on neurons and/or brain tissue? How tedious does this get?
Ellul, M. A., Benjamin, L., Singh, B., Lant, S., Michael, B. D., Easton, A., Kneen, R., Defres, S., Sejvar, J., & Solomon, T. (2020). Neurological associations of COVID-19. The Lancet Neurology, 0(0). https://doi.org/10.1016/S1474-4422(20)30221-0