51 Matching Annotations
- Sep 2019
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Sampling point, Sample Size and Sample collection
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- Jul 2019
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Photography, evaluation and documentation
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Procedurefor SDS-PAGE
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Materialsrequired for SDS-PAGE
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Dinitrosalicylate reagent (DNS)(per liter)
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Identificationof bioagent
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Bacterial strains used
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Production of Lipase
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Extraction of genomic DNA
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- Jun 2019
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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GFP-β-catenin T41A mutant
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Material
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- May 2019
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Cell lysis Buffer
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Fixative
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TAE
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Resuspension solution(Solution I)
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Polydeoxy (Inosinate-cytidylate) (Poly dI-dC)
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Cytoplasmic extractionbuffer (without protease inhibitors)
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Fixative : 4% Formaldehyde
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Cell lysis buffer(RIPA Buffer)
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Phosphate Buffered Saline (PBS)
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Ammonium persulfate(APS)
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Acrylamide (29:1)
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Phenylmethylsulfonyl fluoride (PMSF)
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Benzamidine
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Aprotinin
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Leupeptin
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NP-40ComponentsFinal concentrationFor 10 mlNP-4010%1mlH2O9ml
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Dithiothreitol (DTT)ComponentsFinal concentrationFor 5 mlDTT1.0M0.7725gH2Oq.s
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Ethylenediamine tetraacetic acid (EDTA), pH 8.0ComponentsFinal concentrationFor 500 mlEDTA0.5M93.05gH2Oq.sThe pH is adjusted to 8.0 using 10M NaOH
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Ethylene Glycol Tetraacetic acid (EGTA), pH 7.0ComponentsFinal concentrationFor 50 mlEGTA0.1M1.902gH2Oq.sThe pH is adjusted to 7.0 using 10M NaOH
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Potassium Chloride (KCl)ComponentsFinal concentrationFor 100 mlKCl2M14.91gH2Oq.s
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Sodium Chloride (NaCl)ComponentsFinal concentrationFor 100 mlNaCl5M29.22gH2Oq.s
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Potassium Chloride (KCl)
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HEPES pH 7.9ComponentsFinal concentrationFor 100 mlHEPES1M23.83gH2Oq.sThe pH wasadjusted to 7.9 using 10M NaOH
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Stock solution
Tags
- Mt-2-Mt-1-Mt-9-d
- Mt-2-Mt-4-Mt-1-d
- Mt-2-Mt-10-Mt-1-d
- Mt-2-Mt-7-Mt-1-d
- Mt-2-Mt-1-Mt-6-d
- Mt-2-Mt-1-Mt-5-d
- Mt-2-Mt-3-Mt-1-d
- Mt-2-Mt-1-Mt-8-d
- Mt-2-Mt-8-Mt-1-d
- Mt-2-Mt-1-Mt-13-d
- Mt-2-Mt-1-Mt-1-d
- Mt-2-Mt-1-Mt-3-d
- Mt-2-Mt-1
- Mt-2-Mt-1-Mt-11-d
- Mt-2-Mt-11-Mt-1-d
- Mt-2-Mt-5-Mt-1-d
- Mt-2-Mt-2-Mt-1-d
- Mt-2-Mt-6-Mt-1-d
- Mt-2-Mt-1-Mt-7-d
- Mt-2-Mt-1-Mt-12-d
- Mt-2-Mt-1-Mt-2-d
- Mt-2-Mt-1-Mt-10-d
- Mt-2-Mt-1-Mt-4-d
Annotators
URL
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Extraction buffer
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10XBinding buffer
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Agarose gel
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Nuclear lysis buffer (without protease inhibitors
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Permeabilisation buffer: 0.2% Triton X100
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Stripping buffer
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Blocking buffer
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TBS-T
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Transfer buffer
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(f) Running buffer
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(e) Stacking polyacrylamide gel
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(d) Resolvingpolyacrylamide gel
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(c) 6X Protein loading buffer (Lammeli buffer)
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(b) Celllysis buffer B(For IB)
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Cell lysis bufferA(For IP)
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II. For Immunoprecipitation(IP)and Immunoblotting(IB)
Tags
- Mt-4-Mt-1-Mt-2-Mt-2-d
- Mt-4-Mt-1-Mt-2
- Mt-4-Mt-1-Mt-2-Mt-9-d
- Mt-4-Mt-1-Mt-2-Mt-5-d
- Mt-4-Mt-1-Mt-4-Mt-2-d
- Mt-4-Mt-1-Mt-2-Mt-7-d
- Mt-4-Mt-1-Mt-8-Mt-2-d
- Mt-4-Mt-1-Mt-2-Mt-4-d
- Mt-4-Mt-1-Mt-7-Mt-2-d
- Mt-4-Mt-1-Mt-2-Mt-1-d
- Mt-4-Mt-1-Mt-2-Mt-8-d
- Mt-4-Mt-1-Mt-2-Mt-3-d
- Mt-4-Mt-1-Mt-5-Mt-2-d
- Mt-4-Mt-1-Mt-3-Mt-2-d
- Mt-4-Mt-1-Mt-2-Mt-6-d
Annotators
URL
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