This preprint nicely confirms our original cryo-electron tomograms revealing the fishnet slit diaphragm ( https://www.kidney-international.org/article/S0085-2538(25)00759-8/fulltext ) , first posted here on bioRxiv three years ago ( https://www.biorxiv.org/content/10.1101/2023.10.27.564405v3 ).

The authors interpret the fishnet weaving differently, but at this resolution, multiple models can fit. One key point: in our model, both transmembrane proteins anchor in the podocyte plasma membrane, whereas in the newly proposed model, Neph1 ends ~10 nanometer away - a discrepancy that is difficult to reconcile.

Higher resolution is needed to resolve the true molecular interfaces!

Achilleas Frangakis

phrenes - a word connected in ancient Greece to the idea of respiration and of the soul