Over 20 orthogonal serine integrases have been characterized to date (6,11), and many now have identified RDFs.
5 Matching Annotations
- Oct 2019
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academic.oup.com academic.oup.com
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- Aug 2019
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www.sciencedirect.com www.sciencedirect.com
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unique selling point of the latter systems is their one-way directional nature
Advantages of serine integrases
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attP and attB sites for serine integrases are both short (attP ∼50 bp; attB ∼40 bp); each site is bound by a dimer of integrase, and no host-encoded proteins are required
Advantages of serine integrases
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- May 2019
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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attB and attP sites are typically ≤ 50 bp with different inverted repeat sequences flanking 2 to 12 bp of complementary sequence, which includes the recombination crossover point
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- Oct 2017
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onlinelibrary.wiley.com onlinelibrary.wiley.com
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short flexible glycine–serine linker connects the two scFv domains facilitating bending and/or twisting of the two domains against each other.
glycine-serine linker
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