Application of sample

INSTRUMENTATION

Collection of N.sativaseeds

Cytotoxicity assay by MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyltetrazolium Bromide)method

Collection of N. sativaseeds

Preparation of TLC plate

Test for Sterols

Collection of N.sativaseeds

Preparation of bioformulations and determination of

Sequence analysis

DNA sequencing of the 18S rDNA fragment

Purification of PCR product

Analysis of internal transcribed spacer region

RAPDand SSRscoring and data analysis

PCR amplification

Running of gel and visualization of DNA

Determination of the yield

Agarose gel electrophoresis

Qualitative and quantitative estimation of DNA

Determination of the yield

Procedure for DNA isolation

Reagents required for fungal DNA isolationand p

DNA isolation of Trichodermaisolate

Photography, evaluation and documentation

Procedurefor SDS-PAGE

Materialsrequired for SDS-PAGE

Protein profiling of bioagent through SDS-PAGE

Biochemical analysis (Protein estimation)

Protein estimation through Kjeldahl method

Validations

Mapping of SbDof genes on sorghum chromosomes and its intron/exon gene structure prediction

Sequencing PCR

Minipreparation of plasmid DNA from transformed co

Restriction digestion

Basic requirements for PC

DNA extraction procedure

Germination of seeds

Solutions used for cytokine assay

Assessment of Lymphocyte proliferative responses (LTT) in cured/exposed patients and hamsters

Parasites

Amplification, Cloning and Sequencing

Transformation procedure

Preparation of master plate and isolation of plasmid DNA from transformed E. coli (Mini Prep)

Preparation of chemically competent Escherichia coli using calcium chloride method

Estimation of Total protein

MTT [(4, 5–dimethylthiazol–2–yl)–2, 5-diphenyl tetrazolium Bromide] assay

Kirby-Bauer Disk diffusion method

DPPH free radical quenching activity

UV–visible spectral analysis

Collection and preparation of extracts

Algorithm

Data Sources

Poisson Model

We have analysed the Cancer data of patients in 155 wards of Chennai Cor-poration by the above described method. As preliminary analyses, we havecreated the Choropleth maps for Observed counts, Population of wards, ex-pected counts for patients and SMR's.The Choropleth map for the observed counts Figure 5.2 does not show anypattern. But the Choropleth map for the expected counts Figure: 5.4 indi-cate that the inner regions of the Chennai Corporations have lower expectedcounts and the regions along the border have larger counts of patients. As ameasure of spatial heterogeneity we have computed PSH= 0:7108:Hence ofthe total spatial random variation, nearly 71% is due to spatial heterogene-ity and the remaining 28:92% is due to Poisson variation. Thus the spatialvariation is present in the data.The Choropleth map for Empirical Bayes smoothed rates Figure 5.5 re-veals that only 13 sub regions have high risk values. The wards with numbers53, 64, 67, 70, 78, 93, 100, 103, 110, 117, 122, 147 and 151 have high riskvalues. Though this information could be used by the health managers toconcentrate their work on these regions, one can look for additional covariatesin these regions for further study

Empirical Bayesian Smoothing

Incidence Rate and SMR

Inhibition of haemocytes spreading behavior

. Inhibition of haemocytes aggregation behavior

Haemolymph protein profiling

Total haemocyte count (THC)

Haemolymph collection

Asparate (AAT) and Alanine aminotransferase (ALT)

Insect collection

Starvation and collection method

Insects Collection

Gross morphology and histology of salivary gland

Morphometry of head and stylet

Head and stylet preparation

Insect collection and maintenance

SDS -PAGE for serum protein analysis

Succinic dehydrogenase

Acid Phosphatase

Protein content

Characteristic features ofLabeo rohita

Survey of lakes to check their status of pollution

Experimental Set up

Mechanical composition

Experiment 1: Assessing the impacts of elevated CO2and N levels on yield and nutrient uptake in rice

Temperature Gradient Tunnels (TGT)

Free Air Carbon dioxide Enrichment (FACE)Facility

Site

Estimation of total N% of wheat grainsand straw

Chlorophyllcontent

Root length (cm) and Root weight (mg)

Coleoptile length(cm)

Stomata / cm2

Leaf area index (LAI)

Physiological parameters

Normalized difference vegetation index (NDVI)

Spike length (cm)

Last node to spike length(cm)

Peduncle length(cm)

HarvestIndex

Grain yield per plot (g)

Biological Yield(g)

1000 Kernel weight(mg)

Number of grains per spike

Number of productive tillers per meter

Plant height (cm)

Days to physiological maturity

Days to heading

Field observations

Inoculum concentration

STUDY AREA

Healthy Controls

1X PBS

Generation of polyclonal antibodies against GFP, APC and β-catenin

Cell culture and drug treatment

Bacteria

Reagent

Reagent

Reagent

Reagents

Reagents

Extraction and determination of ascorbic acid

Standard curve of ascorbic acid

Reagents

Estimation of ascorbic acid

Root and Shoot length

ZnSO4

Seeds and germination

Genomic DNA extraction

MN Blood group typing

Reagents

Reagents

Isolation and culture of HUVECs

Procedur

Reagents

Nitric oxide radical scavenging activity

Procedur

Reagents

Hydroxyl radical scavenging assay

Procedure

Reagent

Superoxide anion scavenging activit

Procedure

Reagent

Total antioxidant activit

Procedur

Reagen

Free radical scavenging activity

In VitroStudies

Materials

Material

Total alkalinity

Dissolved oxygen and Biological oxygen demand

Total dissolved solids

Multi parameter analyzer

Characterization of embelin isolated from E.ribes

. Extraction and isolation of embelin from E. ribes

High Performance Liquid Chromatography (HPLC) analysis

High Performance Thin Layer Chromatography (HPTLC) analysis

. Thin Layer Chromatography (TLC) analysis

Preliminary phytochemical screening (Ali, 1998; Evans, 2002)

Microbial Contamina

Foaming Index

pH values

Ash values

Extractive value

. Physico-chemical standardization

Standardization of ethanolic extract of E.ribes (WHO, 1998; IndianPharmacopoeia, 1996)

Preparation of ethanolic extract of E.ribes

PLANT MATERIAL

Estimation of Stevioside

Extraction from the plant material

Extraction from the plant material

Extraction from the plant material

Qmmtification of stevioside

Estimation of Steviol

Extraction from plant material

Quantification of steviol

Estimation of free aminoacids

Estimation of soluble proteins

Estimation of sugars

Estimation of total phenols

Determination of moisture

Analytical methods

The experimental part of the study was categorized into five sections for the convenience of reference viz. analytical, toxicological, molecular, biochemical and genomic quantitation.

Per-cent Overlap

Compogram

F-and t-Ratios

Chi-Square

Somatotype Categories

Comparative Statistics

Somatotype Attitudinal Mean (SAM)

Somatotype Attitudinal Distance (SAD)

Somatotype Dispersion Mean (SDM)

Somatotype Dispersion Distances (SDD)

Somatoplot Coordinates

Mean Somatotypes (5)

Descriptive Statistics

Statistical Analysis

Central:

Mesomorphic ectomorph:

Endomorphic ectomorph:

Ectomorphic mesomo~ph:

Endomorphic mesomorph:

Ectomorphic endomorph:

Mesomorphic endomor~:

Endomorph-ectom9££E: