C = [Protein]*N/KD
C-value
C = [Protein]*N/KD
C-value
Binding dominated by hydrophobic interactio
binding dominated by hydrophobic interaction
DMSO has high heats of dilution and should be matched extremely well between the cell and the syringe
DMSO
The two binding partners must be in identical buffersto minimize heats of dilution
must be in identical buffer to minimize dilution heat