Donor and recipients cells were mixed 1:1 (vol/vol, 0.1 to 1 ml), pelleted (2,000g for 10 min), resuspended in 10–100 µl LB + 300 µM DAP, pipetted onto a LB + 300 µM DAP plate and incubated at 37 °C for 2 h
conjugation with DAP
2 Matching Annotations
- Jun 2022
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www-nature-com.ezproxy.rice.edu www-nature-com.ezproxy.rice.edu
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- Sep 2020
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barricklab.org barricklab.org
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Mu Free Donor (MFDpir) or B2155, which contain integrated rp4-based transfer machinery
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