- Dec 2024
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www.jthjournal.org www.jthjournal.org
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Molecular findings:
Severe bleeding phenotype in homozygotes, alleviated bleeding phenotype in heterozygotes.
Difficult to classify based on the phenotype and plasma levels.
p.M771V variant shows elevated proVWF to mature VWF ratio, reduced levels of cleaved VWFpp.
p.M771V has potential to negatively influence VWFpp cleavage to contribute to decreased VWF processing in endothelial cells.
Other variants near p.M771 locus reported to disrupt the cleavage by furin. (Variants are at R763 and R760 respectively).
Other researchers have come across the p.M771 variant and reported it as well.
functional studies performed in cord blood derived ECFCs through adenine base editing and overexpressed in HEK293 cells.
Utilized Immunocytochemistry staining and confocal analysis
ELISA to test for secreted VWF in ECFCs
Multimer assay and densitometry graphs to view VWF multimer patterns
Western blotting to view proteolytic processing of VWF and VWFpp
Genomic sequencing to verify mutations.
Authors here used SpCas9-mediated base editing to mimic patient variant if primary ECFCs are not available or are difficult to culture to assist in improved characterization in a true endothelial background.
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- Apr 2022
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mediated world.
mediated=intermediated=media?
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- Jun 2021
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psyarxiv.com psyarxiv.com
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Read, K., Gaffney, G., Chen, A., & Imran, A. (2021). The Impact of COVID-19 on Families’ Home Literacy Practices with Young Children. PsyArXiv. https://doi.org/10.31234/osf.io/dvcqm
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- Feb 2020
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journals.sagepub.com journals.sagepub.com
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Discourses tend to be intertextual and interdiscursive (Reisigl and Wodak, 2001: 39). They interlink various texts, discourses and contexts. Social media data are therefore not independent from other media but tend to be multimodal and connected with texts in traditional media. An example is that many political tweets tend to link to articles in the online versions of mainstream newspapers. Studying social media therefore does not substitute the study of other media but often requires studying various media’s intercon-nection. Discourses are texts that stand in particular societal, political-economic, histori-cal, cultural contexts. Understanding them requires taking a holistic point of view, that is, to situate them in history and society.
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- Aug 2018
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Earlier communication tools enabled individuals to create a private list ofcontacts (for instance a buddy list on instant messaging), to establish a group of contactsthat were shared by others (such as a listserv membership list), or to publish a list ofrelated links (such as a blogroll), but SNSs extended the practice of creating a publiclyvisible, personally curated list of contacts and made it a mainstream practice.
Differences between SNS and CMC.
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