12 Matching Annotations
  1. Jun 2023
    1. To date, serine integrases have not been used extensively in plant systems

      although they have been shown to work in principle in Arabidopsis23, Nicotiana benthamiana2421, barley25 and wheat26

  2. Dec 2019
  3. Oct 2019
    1. in the absence of tamoxifen, it exhibits some activity
    2. A technique common in rodents is the use of Cre recombinase lines that are inducible at specific developmental time points (Figure 3b). The most common form of inducible Cre is CreERT2, which contains a modified estrogen receptor binding domain that prevents Cre from entering the nucleus in the absence of a ligand
    3. strong promoters capable of driving expression of microbial opsins or fluorescent proteins in specific populations can exhibit leaky expression elsewhere. This low-level leak may be virtually undetectable as light responsiveness or fluorescence but can be a serious issue when expressing Cre recombinase.
  4. Aug 2019
    1. uses of serine integrases for synthetic biology applications; for assembly of long arrays of genes or other DNA fragments, creation of genetic switches, circuits and logic systems, and for permanent genomic recording of cellular events

      Serine integrase applications

    2. unique selling point of the latter systems is their one-way directional nature

      Advantages of serine integrases

    3. attP and attB sites for serine integrases are both short (attP ∼50 bp; attB ∼40 bp); each site is bound by a dimer of integrase, and no host-encoded proteins are required

      Advantages of serine integrases

  5. May 2019
    1. attB and attP sites are typically ≤ 50 bp with different inverted repeat sequences flanking 2 to 12 bp of complementary sequence, which includes the recombination crossover point
    1. using recombinases can be challenging because their reactions are slow (requiring 2–6 h) and often generate mixed populations when targeting a multicopy plasmid