Commemorated as the Stalin Dam on October 10, 1932, the Dnepr River dam remains the largest electricity producer in Ukraine today.

hese rehab facilities the these addiction treatment centers they they they CL 85% of them in the US are based on the disease model 85% and an almost overlapping 85% uses 12-step methods as their primary primary uh um uh intervention method well you know that's hard to actually figure out because medicine is this and 12 steps has very little to do with medicine it's kind of based on a religious orientation

Most people in America today (85–90%) agree on most issues and topics (85–90%). The so-called polarization is the result of a media landscape that amplifies the voices of the 10–15% that keep constantly talking about the 10–15% of topics on which people are not on the same page.

Cells reconstituted with WT-PALB2 showed substantially less sensitivity to olaparib than cells expressing p.A1025R and p.I944N (Fig. 4a). Similar results were observed for cisplatin treatment, although the difference in sensitivity was less pronounced (Fig. 4b). p.L24S, p.L1070P, and p.L35P were also associated with greater sensitivity to olaparib (Fig. 4c) and cisplatin (Fig. 4d) than WT-PALB2.

Cells reconstituted with WT-PALB2 showed substantially less sensitivity to olaparib than cells expressing p.A1025R and p.I944N (Fig. 4a). Similar results were observed for cisplatin treatment, although the difference in sensitivity was less pronounced (Fig. 4b). p.L24S, p.L1070P, and p.L35P were also associated with greater sensitivity to olaparib (Fig. 4c) and cisplatin (Fig. 4d) than WT-PALB2.

WT-PALB2 was associated with robust formation of damage-induced RAD51 foci, whereas the four variants were associated with defective foci formation (Fig. 3d, e).

Results for individual PALB2 variants were normalized relative to WT-PALB2 and the p.Tyr551ter (p.Y551X) truncating variant on a 1:5 scale with the fold change in GFP-positive cells for WT set at 5.0 and fold change GFP-positive cells for p.Y551X set at 1.0. The p.L24S (c.71T>C), p.L35P (c.104T>C), p.I944N (c.2831T>A), and p.L1070P (c.3209T>C) variants and all protein-truncating frame-shift and deletion variants tested were deficient in HDR activity, with normalized fold change <2.0 (approximately 40% activity) (Fig. 1a).

A total of 84 PALB2 patient-derived missense variants reported in ClinVar, COSMIC, and the PALB2 LOVD database were selected

The trick is to work at 85% capacity rather than 100%. It can be surprisingly challenging to take your effort down a notch and keep it there — especially right now when so many livelihoods feel precarious. But going full-throttle all the time actually works against you. When your mind is relaxed, you’re able to produce better, more thoughtful results.