13 Matching Annotations
  1. Oct 2022
    1. Additionally, make sure to use both forward and side scatter on log scale when measuring microparticles or microbiological samples like bacteria. These types of particles generate dim scatter signals that are close to the cytometer’s noise, so it’s often necessary to visualize signal on a log scale in order to separate the signal from scatter noise.
  2. Mar 2022
    1. Bellesi, S., Metafuni, E., Hohaus, S., Maiolo, E., Marchionni, F., D’Innocenzo, S., La Sorda, M., Ferraironi, M., Ramundo, F., Fantoni, M., Murri, R., Cingolani, A., Sica, S., Gasbarrini, A., Sanguinetti, M., Chiusolo, P., & De Stefano, V. (2020). Increased CD95 (Fas) and PD-1 expression in peripheral blood T lymphocytes in COVID-19 patients. British Journal of Haematology, 191(2), 207–211. https://doi.org/10.1111/bjh.17034

  3. Nov 2021
    1. Side scatter measurement provides information about the internal complexity (i.e. granularity) of a cell. The interface between the laser and intracellular structures causes the light to refract or reflect. Cellular components that increase side scatter include granules and the nucleus (1). 
    2. FSC intensity is proportional to the diameter of the cell, and is primarily due to light diffraction around the cell.
  4. Oct 2021
    1. openCyto package is designed to facilitate the application of automated gating methods in a sequential way to mimic the construction of a manual gating scheme.
  5. Jun 2021
    1. Bacterial cells are typically one thousandth the volume of mammalian cells, which places them near the edge of instrument detection. At this size it can be challenging to differentiate viable cells from debris of similar size
  6. Dec 2020
    1. flow cytometry data were analyzed in R using the flowCore Bioconductor package (
    2. flow cytometry using the red fluorescent nucleic acid dye SYTO17 as a counterstain to improve detection of cells with a low GFP signal
  7. Jan 2020