16 Matching Annotations
  1. Jan 2024
    1. Fluorescence Activated Cell Sorting (FACS).  From sorting cells for single cell genomics to identifying the host range of a plasmid; from detecting metabolically active cells in an environmental sample to studying colitogenic-driving intestinal bacteria, FACS can be used in a multitude of applications to study microbial communities (Rinke et al. 2014, Klumper et al. 2015, Kalyuzhnaya et al. 2008, Hatzenpichler et al. 2016, Palm et al. 2014).
  2. Sep 2023
    1. In one striking demonstration of the potency of the so-called "microbiome-gut-brain axis," published in Gastroenterology in 2011, Bercik and colleagues gave BALB/c mice, a strain of mice that are typically timid and shy, a cocktail of antibiotics, dramatically changing the composition of their gut bacteria. "Their behavior completely changed," Bercik says. "They became bold and adventurous."

      Gut bacteria composition as influencing mind state

      Also, causing to feel an inner flame, or feel suffering instead

  3. Mar 2023
    1. A survey of 230 diverse bacterial and archaeal genomes found evidence of DNA methylation in 93% of genomes, with a diverse array of methylated motifs (834 distinct motifs; average of three motifs per organism)
    1. Bacterial cells are typically one thousandth the volume of mammalian cells, which places them near the edge of instrument detection. At this size it can be challenging to differentiate viable cells from debris of similar size
  4. Oct 2022
    1. Additionally, make sure to use both forward and side scatter on log scale when measuring microparticles or microbiological samples like bacteria. These types of particles generate dim scatter signals that are close to the cytometer’s noise, so it’s often necessary to visualize signal on a log scale in order to separate the signal from scatter noise.
  5. Feb 2022
  6. Feb 2021
  7. Aug 2020
    1. Qu, J., Cai, Z., Liu, Y., Duan, X., Han, S., Zhu, Y., Jiang, Z., Zhang, Y., Zhuo, C., Liu, Y., Liu, Y., Liu, L., & Yang, L. (2020). Persistent bacterial coinfection of a COVID-19 patient caused by a genetically adapted Pseudomonas aeruginosa chronic colonizer. BioRxiv, 2020.08.05.238998. https://doi.org/10.1101/2020.08.05.238998

  8. Jul 2020
  9. Apr 2020
    1. The genetic plurality is passed horizontally among bacteria in the wound, adding to the resistance to treatment and allowing the bacteria to avoid the defense mechanisms of the host such as bacterial phagocytosis, neutrophil degranulation, and formation of reactive oxygen species.
  10. Mar 2020
    1. selected after transformation and used to inoculate liquid cultures

      Would the variability be the same if the colonies were picked from a streaked plate (from glycerol stock) instead of a transformation plate?

      I would expect the glycerol stock method to have more variability, but it would be good to test

  11. Apr 2018
  12. Feb 2018
    1. Some bacteria will swim toward chemicals that suggest a potential reward, such as food.

      Interesting that we could influence the behavior of bacteria.

  13. Sep 2017
    1. In space, bacteria “shapeshifts” to defend itself against antibiotics, experiments on board the International Space Station (ISS) have revealed. The discovery potentially poses a big problem for future space travel—as long duration missions happen more frequently, we will need antibiotics to treat sick astronauts. But if space bacteria is able to quickly and effectively develop resistance, common infections could become deadly.

      This is alarming!

  14. Oct 2016
    1. Shu Lam, a 25-year-old PhD student at the University of Melbourne in Australia, has developed a star-shaped polymer that can kill six different superbug strains without antibiotics, simply by ripping apart their cell walls.

      ...

      Before we get too carried away, it's still very early days. So far, Lam has only tested her star-shaped polymers on six strains of drug-resistant bacteria in the lab, and on one superbug in live mice.

      http://www.nature.com/articles/nmicrobiol2016162