Insect collection and maintenance

GROSS MORPHOLOGY OF THE REDUVIID HEAD AND SALIVARY GLAND COMPLEX

SDS -PAGE for serum protein analysis

Succinic dehydrogenase

Acid Phosphatase

Protein content

Characteristic features ofLabeo rohita

Survey of lakes to check their status of pollution

Growth maximum values

Rates of growth (OD678/day

Growth

Experimental Set up

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Mechanical composition

Experiment 1: Assessing the impacts of elevated CO2and N levels on yield and nutrient uptake in rice

Temperature Gradient Tunnels (TGT)

Free Air Carbon dioxide Enrichment (FACE)Facility

Site

Phenological traits and plant height

Analysis of variance and differences among wheat varieties released in different years in India

Estimation of total N% of wheat grainsand straw

Chlorophyllcontent

Root length (cm) and Root weight (mg)

Coleoptile length(cm)

Stomata / cm2

Leaf area index (LAI)

Physiological parameters

Normalized difference vegetation index (NDVI)

Spike length (cm)

Last node to spike length(cm)

Peduncle length(cm)

HarvestIndex

Grain yield per plot (g)

Biological Yield(g)

1000 Kernel weight(mg)

Number of grains per spike

Number of productive tillers per meter

Plant height (cm)

Days to physiological maturity

Days to heading

Field observations

Inoculum concentration

STUDY AREA

Tested individually

T cell frequencies(Post vaccination response)

Decreased CD3 ζ chain expression on CD8 T cells in HBsAgpositive newborns

T cell phenotypic distribution in HBsAgPositive, HBsAgNegative from HBsAg positive mothers and healthy newborns.

Clinical characteristics of the subjects

Healthy Controls

1X PBS

Generation of polyclonal antibodies against GFP, APC and β-catenin

Primary antibodies

pGEX6P2-BPN (1-993 a.a)

pET30a-GFP-FL (GFP antigen)

pET30-b-APC Nde/Sca (1211-1495 a.a) (antigen for APC antibody)

pET30a-β-catenin-FL (Antigen for -catenin antibody)

GFP-β-cateninC-C (423-634 a.a) (ARM-C)

GFP-β-catenin-C-N (151-423 a.a) (ARM-N)

GFP-β-catenin-C (ARM)

GFP-β-catenin- C (634 -781 a.a)

GFP-β-catenin- a.a

GFP-β-catenin T41A mutant

Constructs made in the study

GFP-β-catenin-FL

passer

Cell culture and drug treatment

Commonly used Buffers/Reagents

IL-2 Bioassay

IL-2 Bioassay

IL-2 Bioassay

IL-2 Bioassay

Total Anti-Stm and anti-E coli antibody assay

Bacteria

Reagents

2 Bioassay

Reagent

Reagent

Reagent

Reagents

Reagents

Extraction and determination of ascorbic acid

Standard curve of ascorbic acid

Reagents

Estimation of ascorbic acid

Root and Shoot length

ZnSO4

Seeds and germination

Phosphate buffered saline (PBS), 0.15M, pH 7.2

Bacterial load in the spleens studied by cfu plating

Expression of costimulatory molecules on the vaccines

Animals

Genomic DNA extraction

Luria-Bertani (LB) medium

MN Blood group typing

MN Blood group typing

Reagents

Reagents

Isolation and culture of HUVECs

Instruments used for the study

MATERIALS

Procedur

Reagents

Nitric oxide radical scavenging activity

Procedur

Reagents

Hydroxyl radical scavenging assay

Procedure

Reagent

Superoxide anion scavenging activit

Procedure

Reagent

Total antioxidant activit

Procedur

Reagen

Free radical scavenging activity

In VitroStudies

Chemicals

Materials

Material

Materials

Material

Total alkalinity

Dissolved oxygen and Biological oxygen demand

Total dissolved solids

Multi parameter analyzer

MATERIALS AND METHODS

PHYSICO-CHEMICAL PARAMETERS OF SEAWATER

Characterization of embelin isolated from E.ribes

. Extraction and isolation of embelin from E. ribes

High Performance Liquid Chromatography (HPLC) analysis

High Performance Thin Layer Chromatography (HPTLC) analysis

. Thin Layer Chromatography (TLC) analysis

Preliminary phytochemical screening (Ali, 1998; Evans, 2002)

Microbial Contamina

Foaming Index

pH values

Ash values

Extractive value

. Physico-chemical standardization

Standardization of ethanolic extract of E.ribes (WHO, 1998; IndianPharmacopoeia, 1996)

Preparation of ethanolic extract of E.ribes

PLANT MATERIAL

Estimation of Stevioside

Extraction from the plant material

Extraction from the plant material

Extraction from the plant material

Qmmtification of stevioside

Estimation of Steviol

Extraction from plant material

Quantification of steviol

Estimation of free aminoacids

Estimation of soluble proteins

Estimation of sugars

Estimation of total phenols

Determination of moisture

Analytical methods

The experimental part of the study was categorized into five sections for the convenience of reference viz. analytical, toxicological, molecular, biochemical and genomic quantitation.

Plant material

e5 tevia-the natural smeetenei

Mean Somatotypes:

Mean Somatotypes:

Mean Somatotypes:

Somatotype Categories:

Range of Component Ratings:

Somatocharts:

Mean Somatotypes:

SOMATOTYPE DISTRIBUTION OF URBAN SAMPLE

Per-cent Overlap

Compogram

F-and t-Ratios

Chi-Square

Somatotype Categories

Comparative Statistics

Somatotype Attitudinal Mean (SAM)

Somatotype Attitudinal Distance (SAD)

Somatotype Dispersion Mean (SDM)

Somatotype Dispersion Distances (SDD)

Somatoplot Coordinates

Mean Somatotypes (5)

Descriptive Statistics

Statistical Analysis

Central:

Mesomorphic ectomorph:

Endomorphic ectomorph:

Ectomorphic mesomo~ph:

Endomorphic mesomorph:

Ectomorphic endomorph:

Mesomorphic endomor~:

Endomorph-ectom9££E:

Mesomorph-endomorph:

Mesomorph-endomorph:

Balanced ectomorph:

Balanced mesomorph:

Balanced endomorph:

SOHATOCHART

Somatotyping Children

Limitations of the Rating Form

Third Component Rating

Second Component Rating