1. Last 7 days
    1. that they might not observe what he was doing.

      It has shown so far that they are not against their religion, is there something that has not been shown which causes him to hide his prayer? Different groups of Natives = different ideas? Again, how reliable is Mary as a narrator

    2. I thought I never tasted pleasanter meat in my life

      Again, adapting to their food, in contrast to when she refused to even try their meat. She’s basically trading here, services for food.

    3. could not but be amazed at the numerous crew of pagans that were on the bank on the other side.

      Is Pagan meaning the same thing that I might currently think of in this text? Why is she SO amazed, is it good or bad?

    4. . I offered the money to my master, but he bade me keep it; and with it I bought a piece of horse flesh

      She’s finally accustomed to their food, buying horse meat on purpose. Nice of her master to allow her to keep the shilling. She sees them as heartless, but never looks deeper.

    5. yelling and hooting

      Still seems kind of offensive but doesn’t seem as harsh as a previous description she created. Maybe she is getting used to it and not seeing it as crazy and barbaric

    6. Yet I answered, they would kill me. “No,” said he, “none will hurt you.” Then came one of them and gave me two spoonfuls of meal to comfort me, and another gave me half a pint of peas; which was more worth than many bushels at another time.

      Assumption vs reality. Don’t assume the worst and you may be helped. Assume the worst and you may be pleasantly surprised.

    7. Then my heart began to fail: and I fell aweeping, which was the first time to my remembrance, that I wept before them.

      Why is she crying only now in front of them? Finally too much to bear? Must be a significance in it being NOW and now when her child died, being hurt, or any other moment in her captivity

    8. I observed they asked one another questions, and laughed, and rejoiced over their gains and victories.

      She’s seeing the human (in her view) in them

    9. to declare the works of the Lord, and His wonderful power in carrying us along, preserving us in the wilderness, while under the enemy’s hand, and returning of us in safety again

      Preserving in the wilderness (with the Natives) and believing he will eventually bring them back to safety (English men)

    10. Naked came I out of my mother’s womb, and naked shall I return: the Lord gave, the Lord hath taken away, blessed be the name of the Lord.

      You always leave the world the way you come into the world?

    11. Job

      Reading something like this is sometimes so difficult because I know very little religious references so before I saw the bible quote and guessed what it was, I was very confused when I saw the word “job”

    12. my son Joseph unexpectedly came to me. We asked of each other’s welfare, bemoaning our doleful condition,

      Oh hello Joseph? Where is he coming from and is he “free”

    13. river, I must go four or five miles up the river farther northward

      Is she going alone or with people? If she is alone what is stopping her from leaving? Not knowing her surroundings?

    1. rhetoric as the ability to use language or images to communicate a message.

      being able to persuade someone with images can be good or bad depending on what message they are trying to communicate.

    1. eLife Assessment

      This important study shows that self-supervised pretraining on related fungal genomes can substantially improve sequence-to-expression prediction in budding yeast. The evidence for the main conclusion is convincing, with careful comparisons to models trained from random initialization and clear gains on held-out expression data. The broader claims about the optimal evolutionary scope and transfer of regulatory grammar are less fully supported.

    2. Reviewer #1 (Public review):

      Summary:

      The authors ask whether self-supervised pretraining on related fungal genomes gives a useful prior for predicting gene expression in S. cerevisiae, where the compact ~12 Mb genome supplies too few independent windows to train a large supervised model from scratch. They pretrain a BERT-style masked DNA language model on a corpus of fungal genomes and fine-tune it to predict RNA-seq gene expression. They found a surprisingly (to me) large improvement in performance: 0.78 prediction Pearson R versus 0.67 for a randomly initialized model.

      The manuscript also presents a new experimental resource: 3,053 RNA-seq data sets with perturbations using the YETI experimental platform to upregulate specific genes.

      Strengths:

      (1) Overall, the manuscript is well-written and is likely to be impactful.

      (2) The protocol handles the train/test split of orthologous sequences well, which is nontrivial.

      (3) The authors did a good job "steelman-ing" Shorkie_Random_Init: it received its own learning-rate sweep, and the authors tested two reduced-capacity from-scratch architectures to rule out some overparameterization issues.

      (4) The public codebase is unusually well-organized.

      Weaknesses:

      I have a number of comments, none of which significantly impact the main findings.

      Two analyses appear missing from the MPRA section: (1) Does self-supervised pre-training improve MPRA models such as DREAM-RNN? and (2) Is Shorkie better than Shorkie_Random_Init at the MPRA task? The language about "correlative, non-causal" associations makes me think the authors tried this and got poor results; it would be informative to include these as a supplementary negative result. (There is also (3): Does MPRA pre-training improve genomic models? But this is clearly out of scope for this paper.)

    3. Reviewer #2 (Public review):

      Summary:

      Chao et al. study whether multi-species self-supervised pretraining learns sequence representations that can be transferred to improve downstream expression prediction in budding yeast. The rationale is that the budding yeast genome is relatively small and compact, which may not provide enough sequence variation for standard reference-genome-based supervised learning.

      This paper has two stages. They first train U-Net-style DNA language models on collections of fungal genomes with increasing phylogenetic breadth using a BERT-style masked language modeling (MLM) objective. The model with the best sequence reconstruction (i.e., lowest perplexity) on held-out budding yeast sequences is called Shorkie LM. They then use Shorkie LM's weights to initialize Shorkie, a supervised model for predicting functional genomic tracks (RNA-seq, ChIP-exo, ChIP-MNase) in budding yeast. Compared with a randomly initialized model with the same architecture, Shorkie clearly performs better on held-out expression prediction and outperforms the randomly initialized model on all three variant effect benchmarks. This is good evidence that the pretraining is beneficial. For Shorkie LM and Shorkie, the authors also use interpretation methods to analyze motifs captured across a range of loci, and the results seem to be consistent with known yeast biology.

      Overall, the experiments are well thought out and controlled, and most claims are supported with sufficient evidence. While the idea that pretraining can be beneficial for budding yeast has been reported previously (for scalar expression prediction) [1], this paper's exploration of optimal evolutionary scope and genomic language model pretraining in general still holds practical value for the field. The Shorkie model itself is also a useful resource and ranks first on many tasks in a recent public benchmark for fungal sequence-to-expression models [2]. I only have some minor concerns/suggestions.

      Strengths:

      (1) The authors put considerable effort into evaluating their modeling choices. For the pretraining corpora, they compare four phylogenetic scopes (a species, strain, order, kingdom). Each corpus is evaluated across multiple model architectures/capacities. Extra care is taken with homology filtering between training and held-out data. For supervised transfer, several candidate language models are carried forward to test whether their ranking by language model selection criterion (perplexity) predicts their ranking on the downstream task. In addition, the same-architecture randomly initialized baseline for Shorkie is itself optimized for learning rate, with additional randomly initialized CNN and U-Net models included as additional controls, giving some strong nulls to compare against.

      (2) The experimental dataset generated in this study is also a valuable resource. Using the ministat array, the authors generated ~3,000 time-resolved RNA-seq profiles following transcriptional regulator inductions. Such data are important for understanding transient regulatory events and how they shape the transcriptome over time.

      (3) Several figures include schematic panels that make the whole workflow easy to understand.

      Weaknesses:

      (1) As acknowledged by the authors, the current evolutionary "sweet spot" at 165 Saccharomycetales genomes is potentially confounded by factors like corpus size, annotation quality, and optimization difficulties. I agree that it would be hard to disentangle these factors, but a relatively simple control appears to be missing. Although unlikely, it is still possible that the "sweet spot" is driven partly by the amount of data rather than the phylogenetic scope. One simple control would be to sample 165 genomes from the broader fungal corpus and match the total number of training windows to the Saccharomycetales dataset.

      (2) L148, "These results demonstrate that Shorkie LM captures conserved regulatory grammar." The evidence presented in this section is mainly about recovery of known fungal/yeast sequence motifs, which are more like words than grammar. The latter is usually understood as relationships between motifs such as their spacing, multiplicity, and arrangement. So I suggest the authors either tone down the claim or provide additional analyses that directly test this. One possibility would be to generate nucleotide dependency maps [3] for a handful of loci with well-characterized regulatory syntax.

      (3) Figure 2E. The interpretation of t-SNE clusters might be confounded by the length of genomic elements. The five classes of elements shown in the plot have very different length distributions, but they are extracted and zero-padded to the same input length, and their embeddings are mean-pooled across the full padded sequence. As a result, the fraction of real sequence entering the mean embedding differs substantially across genomic element classes, which could contribute to observed separation independently of learned regulatory features.

      (4) Lines 216-218, "transfer learning from pan-fungal self-supervised pretraining yields generalizable representations of exon-intron structure and regulatory grammar, substantially improving in expression prediction." Similar to point 2, I think this statement is somewhat stronger than what the current evidence supports. The results clearly show that pretraining improves downstream expression prediction, but it is less clear that this improvement can be specifically attributed to the transfer of regulatory grammar and gene structure, rather than motif representations or more general learned inductive biases acquired during pretraining. This is itself an interesting question. It might be possible to get at it by selectively reinitializing the convolution layers vs the transformer blocks while keeping the rest of the Shorkie LM weights, although this could still be hard to interpret if the relevant information is distributed across the model.

      (5) The ISM for Figures 4 and 5 seems to correspond to the summed predicted coverage across all output bins (Methods, Equation 17). I was unclear about the intended goal here. For example, when mutating the ATG42 promoter in Figure 5, is the goal to quantify the predicted effect on ATG42 expression specifically? If so, should the ISM instead be computed by summing only the bins covering the target gene? Otherwise, predicted coverage from neighboring genes could also contribute to the ISM score and affect the comparison across induction time points.

      References:

      [1] Wang Y, Cai Z, Zeng Q, Gao Y, Ouyang J, Xu Y, et al. Genomic Touchstone: benchmarking genomic language models in the context of the central dogma. bioRxiv. Preprint posted online June 30, 2025. doi:10.1101/2025.06.25.661622

      [2] Schneider T. ybench: a benchmark for fungal sequence-to-expression models. GitHub. Accessed August 18, 2026. https://github.com/Tom-Ellis-Lab/yeast-seq2expression-benchmark

      [3] Tomaz da Silva P, Karollus A, Hingerl J, et al. Nucleotide dependency analysis of genomic language models detects functional elements. Nat Genet. 2025;57(10):2589-2602. doi:10.1038/s41588-025-02347-3

    1. as we function within various spheres of activity—economic, po-litical, cultural, educational, professional, recreational—the norms and principleswe follow, the deliberations we engage in, generally don’t refer us to God or to anyreligious beliefs; the considerations we act on are internal to the “rationality” of eachsphere

      Religious morality has become separated from these normal everyday matters, consequently, modern society has been structured in such a way that you can function normally without the need for religion

    2. but this would have been inescapable in earlier centuriesin Christendom.

      Taylor's disdain in this sentence is evident in his use of the word "inescapable", he strongly highlights how times have changed, reducing Christianity to merely one option among many, whereas it used to be present in almost every aspect of life

    1. Valentina enters holding a restoration invoice.

      女主在哪里得到的?前面从未交代,并且也不知道男主在调查什么,只知道他有事瞒着自己

    2. Under cover of adjusting her linen napkin, Valentina’s hand drops beneath the tablecloth. Deftly and quickly, she unbuttons Adrian’s formal trousers, sliding her warm hand under his waistband, pressing her bare skin directly against his sensitive pelvic skin with firm, rhythmic pressure.

      已知女主坐在男主对面,女主怎么能做到隔着桌子解开男主腰带

    3. Adrian folds the note once and slides it inside his jacket. With his other hand, he takes a plain studio-schedule page from a hallway console and places it on the tray where Mara’s note was.

      整个episode15看不懂,不明白男主替换了什么便条,我理解的这里是想表达男主吃醋将什么人传递给女主的便条拿走了,避免女主看见,但是目前看不出来是吃醋新的muse来访还是女主未婚夫

    4. Everyone else looks afraid. He looks ready.

      女主紧接着立刻就问出口了自己内心的疑问,所以这句话内心独白无意义,删除

    1. state of nature = no formal gov + no established political institutions + ppl organise their lives themselves

      civil society = gov exists + formal laws + polotical isntituions => there is a state governign ppl

      Rousseau=> believd that can't assume + state that ALL modern socieites historically developed froma. state of nature => thought that the transition from natural human life -> civil society would take increadibly long time

      Locke = actual groups of ppl could exist in state of nature eg. indigenous groups


      COMPARE what can we ealrn by imagining a world without a sstate ****

      Hobbes State of nature → dangerous and insecure → people need a strong government.

      Locke Natural freedom → problems enforcing rights → people create government → government protects rights AND => if gov fails to protect ppl's natural right/ becomes tyrannical =>ppl have right to change or overthrow it

      Rousseu State of nature → humans are relatively free, peaceful and independent → society introduces inequality and dependence → legitimate political organisation should restore freedom and equality.

    2. Is it possible?

      COMPARE **what can we ealrn by imagining a world without a sstate **

      Hobbes State of nature → dangerous and insecure → people need a strong government.

      Locke Natural freedom → problems enforcing rights → people create government → government protects rights AND => if gov fails to protect ppl's natural right/ becomes tyrannical =>ppl have right to change or overthrow it

      Rousseu State of nature → humans are relatively free, peaceful and independent → society introduces inequality and dependence → legitimate political organisation should restore freedom and equality.

    3. . Thomas Hobbes (1588-1679),

      Hobbes= lived in English Civil war ( england => resemble what he imagined the state of nature be like) => So essentially he asked "What would human life be like without a government capable of maintaining order?" HE wanted ppl to realise =>No government → disorder and insecurity → terrible conditions → therefore, government is necessary.

      • used state o nature as warning to what could happen if political authority disappeared
    4. Was there ever a state of nature? Many philosophers seem reluctant tocommit themselves on this topic. Jean-Jacques Rousseau (1712-78), forexample, thought that so much time would have been required to pass from astate of nature to 'civil society' (a society governed by a formal state) that itwould be blasphemous to assume that modern societies had arisen in this way.He argued that the amount of time needed for the transition was longer than theage of the world, as recorded in the scriptures. Yet, on the other hand, Rousseaualso believed that there were contemporary examples of peoples living in a stateof nature, while John Locke (1632-1704) thought this was true of many groupsliving in seventeenth-century America

      state of nature = no formal gov + no established political institutions + ppl organise their lives themselves

      civil society = gov exists + formal laws + polotical isntituions => there is a state governign ppl

      Rousseau=> believd that can't assume + state that ALL modern socieites historically developed froma. state of nature => thought that the transition from natural human life -> civil society would take increadibly long time

      Locke = actual groups of ppl could exist in state of nature eg. indigenous groups

      Locke = Natural freedom → problems enforcing rights → people create government → government protects rights AND => if gov fails to protect ppl's natural right/ becomes tyrannical =>ppl have right to change or overthrow it

    1. The Zuni in New Mexico tell stories of their origins

      the zuni creation story is a religious explanation of how their people originated and how spiritual beings influence nature.

    2. Native American tribal religious traditions are shaped by the fact that these languages are not written

      native american religions are mainly preserved through oral stpries and rituals rather than written scriptures

    1. deliverance. If we had been God would have found out a way for the English to have passed this river,

      Didn’t she literally have an out or was that for the other woman. Why does she want it now

    2. I told them it was the Sabbath day, and desired them to let me rest, and told them I would do as much more tomorrow; to which they answered me they would break my face

      They will not recognize the Sabbath day. They respect her in her having personal religious beliefs, but they will not spare her a day solely for religion. She has work to do.

    3. and I could starve and die before I could eat such things, yet they were sweet and savory to my taste.

      Beggars can’t be choosers girl, eat up if you want to survive.

    4. did not wet my foot (which many of themselves at the other end were mid-leg deep)

      So she feels it kind of puts her above the others who did get their feet we

    5. Being very faint I asked my mistress to give me one spoonful of the meal, but she would not give me a taste

      The Natives help her but still no food or little food. Very strict on this part.

    6. In this travel, because of my wound, I was somewhat favored in my load; I carried only my knitting work and two quarts of parched meal.

      So even as a captive she was spared the burden of carrying a heavy load during their move. Even as injured I wouldn’t think such kindness

    7. with their old and with their young: some carried their old decrepit mothers, some carried one, and some another.

      All for one and one for all or whatever they say. They all care for each other and help each other

    8. to hold the English army in play whilst the rest escaped

      To hold in play? Is this really meaning in like a playful non serious way? Just temporary. Anyways, why must the rest escape, danger I’m assuming? where do they go/

    1. I read, much of the night, and go south in the winter.

      This line is very interesting because the first line talk about how the warmth and life of April is cruel, and this line talks about how he travels south for the winter. Why would he travel south if he hates the warmth? This paradoxical relationship is puzzling, as though these lines the poem intensely lack life and this one line about heading for warmth and the life of the south just doesn't add up.

    2. I. The Burial of the Dead April is the cruellest month,

      The relationship between the title “The Burial of the Dead” and “April is the cruellest month” became clearer to me after reading The Canterbury Tales and “Burial of the Dead.” One line I originally did not understand in The Canterbury Tales describes the sun as having “half run its course in Aries.” After researching it, I learned that the sun is halfway through the zodiac sign Aries, placing the poem around the middle of April, approximately April 11–17. This date stood out to me because “Burial of the Dead” takes place on Armistice Day, November 11. In the story, people gather to commemorate the casket of an unknown soldier. The casket is treated with great respect, making death seem peaceful, honorable, and worthy of remembrance.

      November 11 and approximately April 11 are exactly five months apart. November marks the beginning of winter, while April marks the real beginning of spring. In “Burial of the Dead,” death and winter allow the unknown soldier to be respectfully buried and remembered. April’s sunlight, however, brings everything back to life and disturbs what has been buried. This helps explain why Eliot calls April “the cruellest month”: death can offer rest, dignity, and remembrance, while returning to life means returning to painful memories and struggles.

      There may also be a connection between Aries and Ares. Although Aries is a zodiac sign and Ares is the Greek god of war, their similar names strengthen the contrast. April represents life returning, but life also means struggle and conflict. In that sense, life can be more like war, while death in “Burial of the Dead” is presented as peaceful, respectful, and even good.

    3. And the dead tree gives no shelter, the cricket no relief, And the dry stone no sound of water. Only There is shadow under this red rock,

      This section particularly has many things in common with the scripts read out at Orthodox Christian burials, as well as the Ezekiel passages. In Ecclesiates 2, there's a passage about how the "almond tree shall flourish, and the grasshopper shall be a burden", whereas here we see that there is a "dead tree", and a "cricket" which both give no relief to the weary in this section of the poem. While not an exact replica of the earlier language, Eliot's version exists as it's described. Part of the "heap of broken images" that he presents to us, they are markedly absent of the God or religion that cause the tree to flower and "grasshopper [to be] a burden". This trend continues into the line "and the dry stone no sound of water. Only There is shadow under this red rock,". This line references the Christian Orthodox funerary prayers that in their prayer to Mother Mary say that God importantly, "He saw Who caused water to gush forth from the rock ... and springs of water for a thirsting people". Again, an act of God solves the "thirsting people"'s struggle, but in Eliot's verse, there is no water to provide sustenance for the implied presence of a thirsting people (or person singular). These lines are also a very potent description of the actual "Waste Land", where God/religion have forsaken the place, and in some ways taken all life with them. Not even a cricket relives the destitute wanderer, and so they can only find shadow's solace from the red rock.

    1. eLife Assessment

      This important study investigates how experimentally introducing two facultative bacterial endosymbionts into the Russian wheat aphid, Diuraphis noxia, affects aphid performance, dispersal, and damage to cereal host plants. The authors provide solid evidence that the two symbionts can generate contrasting phenotypes: Rickettsiella increases plant damage while reducing wing formation and dispersal, whereas Regiella reduces aphid population growth and feeding damage. The successful establishment and stable transmission of these novel symbiont-host associations, combined with experiments spanning individual, whole-plant, population and mesocosm scales, are notable strengths of the work; however, the mechanisms underlying these effects remain unresolved, evidence for horizontal transmission is indirect, and some population-level conclusions rely on relatively small sample sizes or effects that are not consistently detected across time points, and therefore the potential application of these findings to pest management remains promising but speculative. The study will be of broad interest to researchers working on insect symbiosis, plant-insect interactions, and biologically based approaches to pest management.

    2. Reviewer #1 (Public review):

      Summary:

      In this study, the authors examine what happens when two facultative endosymbionts, Rickettsiella viridis and Regiella insecticola, are introduced into a novel aphid host, the Russian wheat aphid (Diuraphis noxia). They ask whether these introduced symbionts affect aphid performance, plant damage, alate production, dispersal, plant defense responses, and symbiont dynamics. The main result is that the two symbionts have contrasting effects: Rickettsiella tends to increase plant damage and reduce dispersal-related traits, whereas Regiella tends to reduce plant damage and aphid population growth, with less evidence for an effect on dispersal.

      Strengths:

      The manuscript presents successful establishment of stable transinfected populations of an agriculturally important aphid species, which is a substantial technical achievement in itself. I also appreciated that the authors examined the system across several experimental contexts, including different host plants, mixed cages at two temperatures, whole-plant assays, and a mesocosm dispersal experiment, rather than relying on a single laboratory setup. Taken together, these experiments provide a useful and reasonably convincing demonstration that novel symbiont associations can generate contrasting phenotypes in this system.

      Weaknesses:

      There are some major aspects of this paper that I thought could be strengthened. My main concern is that the manuscript feels broader than it is conceptually focused. A wide range of outcomes is measured, which gives the study breadth, but it also makes the central question harder to identify. As written, the paper reads more strongly as a proof-of-principle demonstration of ecologically relevant phenotypes than as a tightly framed test of a specific biological idea.

      A second issue is that the biological basis of the reported phenotypes remains less developed than the phenotypic description itself. The authors make a genuine effort to address mechanism through JA, JA-Ile, SA, and metabolomic profiling, but these analyses only partially explain the main results. The negative result for the canonical defense markers is informative, yet it still leaves a substantial gap between the observed variation in plant damage and the processes responsible for it.

      I also think some caution is needed in how the two symbionts are compared. The authors explain why some follow-up experiments were designed differently for Rickettsiella and Regiella, and that rationale is understandable. Still, because the downstream assays were not fully matched, the paper is strongest when each symbiont is interpreted on its own terms rather than as a strict comparison.

      Overall, I would suggest softening the Significance Statement so that it more clearly reflects what is directly shown here, namely that introduced symbionts can alter plant damage and dispersal-related phenotypes under controlled conditions, rather than implying that the study directly tests management utility in agricultural settings.

    3. Reviewer #2 (Public review):

      Summary:

      The authors generated two novel aphid-symbiont associations and examined the impact of these new symbiotic associations on plant-insect-symbiont interactions. The authors notably provide detailed phenotypic assessments of the insect hosts and host plants. They show that one introduced symbiont, Rickettsiella, increases aphid-induced damage to host plants, while the other, Regiella, ameliorates aphid damage. The authors suggest that such novel insect-symbiont pairings may be used as tools to mitigate crop damage in the future.

      Strengths:

      Although a few experiments seem to have limited sample sizes and limited statistical power, these are often complemented with highly replicated smaller-scale experiments. The combination of larger mesocosm and population-level experiments along with assessments of individual insects generally provides a comprehensive depiction of the effects of these symbionts on their hosts. The opposing impacts of Regiella and Rickettsiella infection on the aphid host plant are of broad interest. It is also surprising that the host plants did not exhibit strong differences in canonical defensive signalling, despite these differences.

      Weaknesses:

      One thing that I struggled a little with was the rapid spread of Regiella in the shared plant experiments. Possibly this could be attributed to an increased reproductive output (due to faster developmental time, and/or an increase in fecundity) or efficient horizontal transmission. However, the other experiments performed indicate a slight negative impact (Figure 4a) or no influence (Figure 4C, 4D, Figure S6) of Regiella infection on host fitness. Given these other results, it seems that Regiella must spread fairly efficiently between hosts, which comes as a surprise, and there are very few examples of horizontal transmission of Regiella like this in the literature. The manuscript would benefit from a clear and direct demonstration of horizontal transmission, rather than it being inferred indirectly. The similar spread observed in the Rickettsiella mixed cages is less surprising, because there are several examples where this has been demonstrated.

      It is also a little surprising that mesocosm-dispersal experiments were not also conducted using Regiella-infected lines. At several points throughout the manuscript, the idea of using symbiont transfections to reduce plant harm is raised. I can understand that these experiments are likely time-, space-, and resource-intensive, but that seems like these would have been relevant experiments, especially in the context of controlling damage to plants.

    4. Reviewer #3 (Public review):

      Summary:

      The authors were investigating the impact of introducing novel facultative bacterial endosymbionts into the pest aphid, Diuraphis noxia, to explore the possibility of using facultative symbionts as a crop protection tool. They successfully established the vertical transmission of both endosymbionts and performed a series of aphid performance and dispersal experiments together with measurement of aphid feeding on host plant health, growth, and metabolism. While most of the experiments revealed no effect of the endosymbionts, some significant treatment effects were found, showing that Rickettsiella reduced aphid dispersal, and Regiella reduced aphid population growth and feeding damage.

      Strengths:

      The team worked with two novel facultative symbionts (Rickettsiella viridis and Regiella insecticola) that they were able to successfully establish in D. noxia. The data were collected and analyzed using solid, well-described methodology.

      Weaknesses:

      While interpretation of the data is reasonable, the few experiments which revealed significant treatment effects rest on relatively small sample sizes.

      Measuring symbiont density is difficult. The authors use quantitative PCR to measure the "density" of endosymbionts relative to a host gene. This is a standard approach in the field; however, recent work has shown that endosymbionts like the aphid primary endosymbiont, Buchnera, are variably polyploid [1]; further the aphid cells that house the symbionts are also highly polyploid and variable in their ploidy [2]. It is important to understand that what is being measured when using qPCR is DNA copy number and not quantification of the number of symbiont cells. Alternative approaches to measuring symbiont density include flow cytometry [3], and SymbiQuant [4], a machine vision tool that can quantitatively characterize symbiont populations from DAPI-stained confocal images. These alternate approaches also have their limitations. Currently, there is no perfect approach to measuring symbiont density, which remains an important measure in experiments such as these. Put simply, it is important for a reader to be aware of the limitations of each approach and interpret data accordingly.

      [1] Komaki, K., and H. Ishikawa. 2000. Genomic copy number of intracellular bacterial symbionts of aphids varies in response to developmental stage and morph of their host. Insect Biochemistry and Molecular Biology 30:253-258.<br /> [2] Nozaki, T., and S. Shigenobu. 2022. Ploidy dynamics in aphid host cells harboring bacterial symbionts. Scientific Reports 12:9111.<br /> [3] Simonet, P., G. Duport, K. Gaget, M. Weiss-Gayet, S. Colella, G. Febvay, H. Charles, J. Viñuelas, A. Heddi, and F. Calevro. 2016. Direct flow cytometry measurements reveal a fine-tuning of symbiotic cell dynamics according to the host developmental needs in aphid symbiosis. Scientific Reports 6:19967.<br /> [4] James, E. B., X. Pan, O. Schwartz, and A. C. C. Wilson. 2022. SymbiQuant: A machine learning object detection tool for polyploid independent estimates of endosymbiont population size. Frontiers in Microbiology 13:816608.

      Impact and Significance:

      Food security and production, and pest control are major challenges facing the human population. This work contributes knowledge that will benefit the development of alternate pest control strategies in agriculture.

    1. Many of these secular concerns were also manifested in the world Zionist movement established in 1897 to create a modern Jewish home in the ancient homeland of the Jewish people. Zionism embraced the ideas that a Jewish homeland would serve as a creative center for the revitalization of Jewish cultural values in modern form, that anti-Semitism was a symptom of the abnormality of Jewish life in the Diaspora that could only be cured with Jewish "self-emancipation" made possible by a Jewish commonwealth, and that cooperative farming communities and a vigorous labor movement in the Land of Israel was the expression of a social revolution among the Jewish masses.

      Shows that modern zionism came to be because of antisemitism. The Jewish Israelites became not just a religion but a political and nationalist movement as well. Came to fruition at the establishment of the State of Israel.

    2. By the end of the first century CE, or at least by the late second century, after the last of the Jewish revolts against the Romans, rabbinic Judaism crystallized out of the Pharisaic movement, while Christianity became fully separated from the Jewish people.

      Shows beginnings of Rabbinic Judaism. emphasizes importance of the Torah. Also marks the split of the first two major monotheistic religions.

    3. When Judah was destroyed by the Babylonians in 586 BCE, the explanation offered was that the idolatry of the past, especially the later kings subservient to Assyria, such as Manasseh, had condemned the people to exile but that God continued to love them and held out a sure promise of redemption

      transformed Jewish religious identity - even outside the homeland, the Jewish could remain within the covenant and hold onto their relationship with God

    4. There they entered a binding agreement with their God—a covenant that included a strict prohibition against worshiping other gods

      The covenant between God and the Israelites on Mt. Sinai created the beginnings of Judaism as a religion centered around the relationship between God and the people. Shows connection between the Jews and the land.

    1. RSS is provided by default for WordPress sites, and WordPress is the site builder for 37% of the web. Without WordPress’ support, I don’t think RSS would still be around.

      One thing that bugs me about home-built homepages using static site generators as a consume is that the authors have to choose to have RSS, whereas when I find a good blog that's been around for awhile and it's hosted on WordPress, I can reliably pull in not just the most recent items but a copy of the entire backlog of posts into my feed reader.

    2. RSS is a technology that lets you subscribe to new content on websites, just like you can subscribe to podcasts to get the latest episodes

      RSS isn't "just like [how] you can subscribe to podcasts". RSS is what lets you subscribe to podcasts.

    1. White Man’s Burden

      It goes back to the days of the Indians and boarding schools making them assimilate yet all that means is the white man's burden to civilize the non-whites is creating a burden of dependency on the white man, then they complain they don't want to be burdened anymore.

    1. __________________________________________________________________

      I am known to take the lead. However, it's a group presentation so I would ask that everyone meets up in an hour or two to discuss some team rules and expectations. Then as a team delegate specific areas each person will contribute to. I am comfortable with public speaking so that would be my preferred role. My least favorite role is also my preferred role. I tend to experience cotton mouth. I am open to whatever role makes us the most effective.

    2. What has worked best for me while having to public speak is truly knowing about the topic I am speaking about. If I understand the topic, then I am likely more confident with the information that I am discussing. I haven't done this for class, but I do it in my current role when sharing the programs that I oversee.

    1. Are you a “traditional” or “returning” student? List an important advantage you have as a result of being in this classification:

      I am a returning student because school no longer feels like such a chore, it feels more like a task I've been given to complete and/or given an oppurtunity to make my life easier and better in the long run.

    1. Wait on the Lord, Be of good courage, and he shall strengthen thine Heart, wait I say on the Lord.”

      Ok What does waiting on the Lord get them right now. She has a chance to leave, right? Is this not “god” giving her an out

    2. nine English captives in this place (all of them children, except one woman

      What were they using these captives for/??? Fun? Work? Other duties..?

    3. turn all those curses upon our enemies. I do not desire to live to forget this Scripture, and what comfort it was to me.

      So is she finding comfort in believing that eventually everything bad that the Natives were imposing on her would be turned back on them?

    4. in sending me a Bible.

      A physical manifestation of her hopes and prayers during hardship. Gives her something physical to hold on to and put her faith into. I would imagine this makes it easier for her faith during this time.

    5. Oh! the outrageous roaring and hooping that there was. They began their din about a mile before they came to us. By their noise and hooping they signified how many they had destroyed (which was at that time twenty-three).

      I’m guessing this was houses? 23 houses burnt? Burning homes would certainly be a big roar.

    6. a small plantation of Indians, where it seems he had been during his captivity; and at this time, there were some forces of the Ind. gathered out of our company, and some also from them (among whom was my son’s master) to go to assault and burn Medfield.

      And what would this action DO in this society? also, a plantation?

    7. In this time of the absence of his master, his dame brought him to see me. I took this to be some gracious answer to my earnest and unfeigned desire.

      The dame is not only under the master and has her own free will to act. She is thankful basically too.

    8. my son came to me, and asked me how I did. I had not seen him before, since the destruction of the town, and I knew not where he was,

      How did he know how and where to find her? How did this separation of family and death of a sibling effect the other two children?

    9. He would consider my low estate, and show me a token for good, and if it were His blessed will, some sign and hope of some relief.

      Showing how important religion was/is to some people especially in hardships

    10. When I came in sight, she would fall aweeping; at which they were provoked, and would not let me come near her, but bade me be gone

      So they could see each other but the Natives would not let the mother comfort her crying daughter? Cruel there

    11. I went to see my daughter Mary, who was at this same Indian town, at a wigwam not very far off, though we had little liberty or opportunity to see one another.

      So although they had “little liberty” to see each other, I mean I guess it’s shocking to me that hey could see each other at all and that hey knew where they both were.

    12. Then they went and showed me where it was, where I saw the ground was newly digged, and there they told me they had buried it.

      Honestly, okay, compassion there. They buried the body, not just leaving it somewhere.

    13. went to take up my dead child in my arms to carry it with me, but they bid me let it alone; there was no resisting, but go I must and leave it.

      Wow no sympathy? They made her abandon her child’s lifeless body?

    14. but I was sold to him by another Narragansett Indian, who took me when first I came out of the garrison

      Oh wow did Native Americans also sell white people? I did not know that honestly if this is all true

    15. God to me

      She wanted to kill herself basically, but remained strong within even after her baby died, and says God is the reason why. Strong of her to still find reason in life, even if it is just God. Also, I wonder now, what was the child’s name? Why did we not learn it? Too hard to say or was it more normal to only say “babe?”

    16. I cannot but take notice how at another time I could not bear to be in the room where any dead person was, but now the case is changed; I must and could lie down by my dead babe, side by side all the night after.

      Becomes used to death. and also, i mean, it is her own child so.

    17. picture of death in my lap. About two hours in the night, my sweet babe like a lamb departed this life on Feb. 18, 1675.

      Her baby is the picture of death. Devastating. How old was the child?

    18. This was the comfort I had from them

      Not to like undermine her emotions but honestly if you are captive what can you expect from your captors? Harshness I would think.

    1. § 2  When the people of Athens passed a decree granting the right of citizenship to Pasion and his descendants on account of services to the state, my father favored the granting of the people's gift, and himself gave in marriage to Apollodorus, son of Pasion, his own daughter, my sister, and she is the mother of the children of Apollodorus. Inasmuch as Apollodorus acted honorably toward my sister and toward all of us, and considered us in truth his relatives and entitled to share in all that he had, I took to wife his daughter, my own niece.

      Citizenship creates new kinship relationships: Pasion’s son Apollodorus becomes fully integrated into the speaker’s family through marriage. The passage deliberately emphasizes him as a “relative”, showing how legal citizenship could facilitate social incorporation.

    1. Read the edits Mariah made to make her third paragraph more specific and more concise. Keep in mind, she has already incorporated the changes she made during the revision stage to improve unity and coherence.

      I like the emphasis on conciseness. I tend to talk outloud a lot and this definitely reflects in my writing as well. More often than not, less is more. It communicates more effectively due, as there are less chances for confusion.

    2. • Repetition of the same phrases (“Smith states . . . , Jones states . . .”) to introduce quoted and paraphrased material (for a full list of strong verbs to use with in-text citations, see Chapter 21: Paraphrasing and Quoting)

      This is something I tend to focus a lot on. I have my set words that I gravitate to but dislike how repetition sounds in writing, especially in the same or back to back sentences. I also think variety in word choice projects more intelligent writing.

    3. Read your paper aloud. Sometimes your ears catch inconsistencies that your eyes miss.

      I have used this technique in previous writing classes and find it incredibly helpful! Not only does it allow for awkwardness to become apparent, but it helps with catching physical writing mistakes such as missing the proper ending.

    4. Editing involves re-examining the way you have expressed your ideas and improving word choice (or diction), conciseness, grammar, and tone. You might add, delete, or change words. You’ll fix problems in grammar and sentence structure. You might adjust the tone.

      I wasn't originally sure what the big difference between revision and editing was. I like that revision focuses more on structure and unity whereas revision improves quality of writing. This makes sense to do second in my opinion.

    1. Life in college usually differs in many ways from one’s previous life in high school or in the workforce. What are the biggest changes you are experiencing now or anticipate experiencing this term?

      I actually have to use my time wisely, no more doing whatever I want when I want on the weekends.

    2. Some students say they have known from a very early age what they want to do after college, choose the college that is best for that plan, never waiver from the plan and choose each course with the one goal in mind, and then enter their chosen career after college or graduate school. At the other extreme, some students have only a vague sense of direction before beginning college, take a wide variety of courses, select a major only when they reach the point that they must major in something (or perhaps change majors multiple times), and then after college choose to work in an entirely different field. Some students choose to major in an academic subject simply because they enjoy that subject, never concerned with what kind of job they may get afterward. The traditional idea of the liberal arts education is that you can go to college not to prepare for a specific career but to become a well-educated person who is then in a better position to work in any number of careers. None of these different approaches to choosing a major and a career is better than others. All students receive the many benefits of college, and all are likely to find a more fulfilling career. So where are you in this great variety of attitudes about career and major choices?

      I have only recently achieved an idea of what I want to do with my life and that is to be financially stable enough to raise a family and that starts with a good career.

    3. Do some of the activities you value most contribute to your college experience, or will they distract you from being a good student?

      The activities that I have ranked the highest really have nothing to do with school or being a student in general. All of my hobbies our set in the outdoors and when I'm hanging out with my friends we are 9/10 always outdoors.

    1. Using Transitional Words to Create Coherence

      This section is particularly helpful for me. I think transitional words can absolutely be the difference between cohesion or confusion. I really liked that they gave so many examples and where to use them; this will help add variety within my essay.

    2. When a piece of writing has coherence, the ideas flow smoothly. The wording clearly indicates how one idea leads to another within a paragraph and from paragraph to paragraph.

      Coherence is super important for effective writing. If a reader is confused by a lack of "flow" in ideas, it will lessen the impact of your overall message.

    3. • Set aside your writing for at least a few hours, preferably a day or two, until you can look at it objectively.

      It will be super helpful that there was a week in between when our first draft was due and now as we begin to edit. I tried not to look at my essay during last week and just focus on peer reviews. This time away will definitely give me a new perspective. I wonder how reading other's papers may help my own revision as well.

    4. Revising allows you to examine important aspects of your writing separately so that you can give each task your undivided attention.

      This is a really helpful note. Reflecting on my own essay, there were both stylistic and structural pieces I was not content with. Being able to attack them separately will be less overwhelming, and I believe, more effective.

    1. She is given agency, a development not often explored in popular Indian film.

      When thinking of the storyline, this is really interesting to me that Nisha decides to enter into an arranged marriage. I like that this breaks tradition of her gaining "agency"; it is not forced upon her.

    2. My decision to give my attention to my women characters is a political statement, challenging the predominant culture that has caused them to only exist as personality-less plot devices instead of fully fleshed out entities for hundreds of years.

      I still think this is still societally prevalent. Not a lot of examples come to mind when I think of strong female heroine or lead. Someone like Christopher Nolan is one of the best film producers of our time, and he has never produced a movie with a female protagonist.