Transformation procedure

Preparation of master plate and isolation of plasmid DNA from transformed E. coli (Mini Prep)

Preparation of chemically competent Escherichia coli using calcium chloride method

Genomic DNA isolatio

Cloning, expression and purification ofADH

Results of molecular docking

Receptor X-ray structure

Receptor X-ray Structure

Estimation of Total protein

MTT [(4, 5–dimethylthiazol–2–yl)–2, 5-diphenyl tetrazolium Bromide] assay

Kirby-Bauer Disk diffusion method

DPPH free radical quenching activity

UV–visible spectral analysis

Collection and preparation of extracts

The table 6.1 gives the mixing probabilities and the associated parametricvalues fork(number of components) = 2,3, and 4. It may be noted thatthe Log likelihood value is smaller fork= 4 (the results fork= 5 , 6 etc.are not better than that fork= 4 and hence are not given here). The fourcomponents Poisson Mixture model is given in table 6.2. It may be notedthat 58% of wards may have higher incidence/relative risk and the remainingwards have lesser/lower incidence for the Cancer disease. We computed theposterior probability for each component for each ward (see table 6.3). Eachward is assigned to a particular component so that the posterior probability islarger. These results are also given in table 6.3 Finally we present Choroplethmaps based on those results

Algorithm

Data Sources

Poisson Model

We have analysed the Cancer data of patients in 155 wards of Chennai Cor-poration by the above described method. As preliminary analyses, we havecreated the Choropleth maps for Observed counts, Population of wards, ex-pected counts for patients and SMR's.The Choropleth map for the observed counts Figure 5.2 does not show anypattern. But the Choropleth map for the expected counts Figure: 5.4 indi-cate that the inner regions of the Chennai Corporations have lower expectedcounts and the regions along the border have larger counts of patients. As ameasure of spatial heterogeneity we have computed PSH= 0:7108:Hence ofthe total spatial random variation, nearly 71% is due to spatial heterogene-ity and the remaining 28:92% is due to Poisson variation. Thus the spatialvariation is present in the data.The Choropleth map for Empirical Bayes smoothed rates Figure 5.5 re-veals that only 13 sub regions have high risk values. The wards with numbers53, 64, 67, 70, 78, 93, 100, 103, 110, 117, 122, 147 and 151 have high riskvalues. Though this information could be used by the health managers toconcentrate their work on these regions, one can look for additional covariatesin these regions for further study

Empirical Bayesian Smoothing

Incidence Rate and SMR

Spatial Analysis of Cancer PatientCount Data

Total haemocyte count assay

Spodoptera litura

Insecticidal activity

Inhibition of haemocytes spreading behavior

. Inhibition of haemocytes aggregation behavior

Haemolymph protein profiling

Total haemocyte count (THC)

Haemolymph collection

Asparate (AAT) and Alanine aminotransferase (ALT)

Insect collection

Venomous saliva optimization

Starvation and collection method

Insects Collection

Salivary gland-Histology

Salivary gland-Morphology and Anatomy

Maxillary stylet

Mandibular stylet

Head

Gross morphology and histology of salivary gland

Morphometry of head and stylet

Head and stylet preparation

Insect collection and maintenance

GROSS MORPHOLOGY OF THE REDUVIID HEAD AND SALIVARY GLAND COMPLEX

SDS -PAGE for serum protein analysis

Succinic dehydrogenase

Acid Phosphatase

Protein content

Characteristic features ofLabeo rohita

Survey of lakes to check their status of pollution

Growth maximum values

Rates of growth (OD678/day

Growth

Experimental Set up

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Mechanical composition

Experiment 1: Assessing the impacts of elevated CO2and N levels on yield and nutrient uptake in rice

Temperature Gradient Tunnels (TGT)

Free Air Carbon dioxide Enrichment (FACE)Facility

Site

Phenological traits and plant height

Analysis of variance and differences among wheat varieties released in different years in India

Estimation of total N% of wheat grainsand straw

Chlorophyllcontent

Root length (cm) and Root weight (mg)

Coleoptile length(cm)

Stomata / cm2

Leaf area index (LAI)

Physiological parameters

Normalized difference vegetation index (NDVI)

Spike length (cm)

Last node to spike length(cm)

Peduncle length(cm)

HarvestIndex

Grain yield per plot (g)

Biological Yield(g)

1000 Kernel weight(mg)

Number of grains per spike

Number of productive tillers per meter

Plant height (cm)

Days to physiological maturity

Days to heading

Field observations

Inoculum concentration

STUDY AREA

Tested individually

T cell frequencies(Post vaccination response)

Decreased CD3 ζ chain expression on CD8 T cells in HBsAgpositive newborns

T cell phenotypic distribution in HBsAgPositive, HBsAgNegative from HBsAg positive mothers and healthy newborns.

Clinical characteristics of the subjects

Healthy Controls

1X PBS

Generation of polyclonal antibodies against GFP, APC and β-catenin

Primary antibodies

pGEX6P2-BPN (1-993 a.a)

pET30a-GFP-FL (GFP antigen)

pET30-b-APC Nde/Sca (1211-1495 a.a) (antigen for APC antibody)

pET30a-β-catenin-FL (Antigen for -catenin antibody)

GFP-β-cateninC-C (423-634 a.a) (ARM-C)

GFP-β-catenin-C-N (151-423 a.a) (ARM-N)

GFP-β-catenin-C (ARM)

GFP-β-catenin- C (634 -781 a.a)

GFP-β-catenin- a.a

GFP-β-catenin T41A mutant

Constructs made in the study

GFP-β-catenin-FL

passer

Cell culture and drug treatment

Commonly used Buffers/Reagents

IL-2 Bioassay

IL-2 Bioassay

IL-2 Bioassay

IL-2 Bioassay

Total Anti-Stm and anti-E coli antibody assay

Bacteria

Reagents

2 Bioassay

Reagent

Reagent

Reagent

Reagents

Reagents

Extraction and determination of ascorbic acid

Standard curve of ascorbic acid

Reagents

Estimation of ascorbic acid

Root and Shoot length

ZnSO4

Seeds and germination

Phosphate buffered saline (PBS), 0.15M, pH 7.2

Bacterial load in the spleens studied by cfu plating

Expression of costimulatory molecules on the vaccines

Animals

Genomic DNA extraction

Luria-Bertani (LB) medium

MN Blood group typing

MN Blood group typing

Reagents

Reagents

Isolation and culture of HUVECs

Instruments used for the study

MATERIALS

Procedur

Reagents

Nitric oxide radical scavenging activity

Procedur

Reagents

Hydroxyl radical scavenging assay

Procedure

Reagent

Superoxide anion scavenging activit

Procedure

Reagent

Total antioxidant activit

Procedur

Reagen

Free radical scavenging activity

In VitroStudies

Chemicals

Materials

Material

Materials

Material

Total alkalinity

Dissolved oxygen and Biological oxygen demand

Total dissolved solids

Multi parameter analyzer

MATERIALS AND METHODS

PHYSICO-CHEMICAL PARAMETERS OF SEAWATER

Characterization of embelin isolated from E.ribes

. Extraction and isolation of embelin from E. ribes

High Performance Liquid Chromatography (HPLC) analysis

High Performance Thin Layer Chromatography (HPTLC) analysis

. Thin Layer Chromatography (TLC) analysis

Preliminary phytochemical screening (Ali, 1998; Evans, 2002)

Microbial Contamina

Foaming Index

pH values

Ash values

Extractive value

. Physico-chemical standardization

Standardization of ethanolic extract of E.ribes (WHO, 1998; IndianPharmacopoeia, 1996)

Preparation of ethanolic extract of E.ribes

PLANT MATERIAL

Estimation of Stevioside

Extraction from the plant material

Extraction from the plant material

Extraction from the plant material

Qmmtification of stevioside

Estimation of Steviol

Extraction from plant material

Quantification of steviol

Estimation of free aminoacids

Estimation of soluble proteins

Estimation of sugars

Estimation of total phenols

Determination of moisture

Analytical methods