Spell-Storing Item
Which action is used to activate a Spell-Storing Item? Activating a Spell-Storing Item uses the Use an Object action.
Sage Advice Compendium, v2.7 r2021
Spell-Storing Item
Which action is used to activate a Spell-Storing Item? Activating a Spell-Storing Item uses the Use an Object action.
Sage Advice Compendium, v2.7 r2021
Flash of Genius
What is the timing of Flash of Genius? You use Flash of Genius immediately after the triggering d20 is rolled and before any of the effects of the roll are applied. Unless a rule tells you otherwise, a reaction occurs immediately after its trigger.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Spell-Storing Item
Which action is used to activate a Spell-Storing Item? Activating a Spell-Storing Item uses the Use an Object action.
Sage Advice Compendium, v2.7 r2021
Flash of Genius
What is the timing of Flash of Genius? You use Flash of Genius immediately after the triggering d20 is rolled and before any of the effects of the roll are applied. Unless a rule tells you otherwise, a reaction occurs immediately after its trigger.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Draconic Bloodline
Can a dragonborn sorcerer with a draconic bloodline have two different kinds of Draconic Ancestry? A dragonborn sorcerer can choose a different ancestor for the racial trait and for the Dragon Ancestor feature. Your choice for the racial trait is your actual ancestor, while the choice for the class feature could be your ancestor figuratively— the type of dragon that bestowed magic upon you or your family or the kind of draconic artifact or location that filled you with magical energy.
Sage Advice Compendium v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Extra Attack
When you use Extra Attack, do you have to use the same weapon for all the attacks? Extra Attack imposes no limitation on what you use for the attacks. You can use regular weapons, improvised weapons, unarmed strikes, or a combination of these options for the attacks.
Sage Advice Compendium, v2.7 r2021
Draconic Ancestry.
Can a dragonborn sorcerer with a draconic bloodline have two different kinds of Draconic Ancestry? A dragonborn sorcerer can choose a different ancestor for the racial trait and for the Dragon Ancestor feature. Your choice for the racial trait is your actual ancestor, while the choice for the class feature could be your ancestor figuratively— the type of dragon that bestowed magic upon you or your family or the kind of draconic artifact or location that filled you with magical energy.
Sage Advice Compendium v2.7 r2021
Naturally Stealthy.
Do the lightfoot halfling and wood elf hiding racial traits allow them to hide while observed? The lightfoot halfling and wood elf traits—Naturally Stealthy and Mask of the Wild—do allow members of those subraces to try to hide in their special circumstances even when observers are nearby. Normally, you can’t hide from someone if you’re in full view. A lightfoot halfling, though, can try to vanish behind a creature that is at least one size larger, and a wood elf can try to hide simply by being in heavy rain, mist, falling snow, foliage, or similar natural phenomena. It’s as if nature itself cloaks a wood elf from prying eyes—even eyes staring right at the elf! Both subraces are capable of hiding in situations when most other creatures can’t, but neither subrace’s hiding attempt is assured of success; a Dexterity (Stealth) check is required as normal, and an observant foe might later spot a hidden halfling or elf: “I see you behind that guard, you tricksy halfling!”
Sage Advice Compendium v2.7 r2021
Mask of the Wild.
Do the lightfoot halfling and wood elf hiding racial traits allow them to hide while observed? The lightfoot halfling and wood elf traits—Naturally Stealthy and Mask of the Wild—do allow members of those subraces to try to hide in their special circumstances even when observers are nearby. Normally, you can’t hide from someone if you’re in full view. A lightfoot halfling, though, can try to vanish behind a creature that is at least one size larger, and a wood elf can try to hide simply by being in heavy rain, mist, falling snow, foliage, or similar natural phenomena. It’s as if nature itself cloaks a wood elf from prying eyes—even eyes staring right at the elf! Both subraces are capable of hiding in situations when most other creatures can’t, but neither subrace’s hiding attempt is assured of success; a Dexterity (Stealth) check is required as normal, and an observant foe might later spot a hidden halfling or elf: “I see you behind that guard, you tricksy halfling!”
Sage Advice Compendium v2.7 r2021
Trance.
Does the Trance trait allow an elf to finish a long rest in 4 hours? If an elf meditates during a long rest (as described in the Trance trait), the elf finishes the rest after only 4 hours. A meditating elf otherwise follows all the rules for a long rest; only the duration is changed.
Sage Advice Compendium v2.7 r2021
Draconic Ancestry.
Can a dragonborn sorcerer with a draconic bloodline have two different kinds of Draconic Ancestry? A dragonborn sorcerer can choose a different ancestor for the racial trait and for the Dragon Ancestor feature. Your choice for the racial trait is your actual ancestor, while the choice for the class feature could be your ancestor figuratively— the type of dragon that bestowed magic upon you or your family or the kind of draconic artifact or location that filled you with magical energy.
Sage Advice Compendium v2.7 r2021
Naturally Stealthy.
Do the lightfoot halfling and wood elf hiding racial traits allow them to hide while observed? The lightfoot halfling and wood elf traits—Naturally Stealthy and Mask of the Wild—do allow members of those subraces to try to hide in their special circumstances even when observers are nearby. Normally, you can’t hide from someone if you’re in full view. A lightfoot halfling, though, can try to vanish behind a creature that is at least one size larger, and a wood elf can try to hide simply by being in heavy rain, mist, falling snow, foliage, or similar natural phenomena. It’s as if nature itself cloaks a wood elf from prying eyes—even eyes staring right at the elf! Both subraces are capable of hiding in situations when most other creatures can’t, but neither subrace’s hiding attempt is assured of success; a Dexterity (Stealth) check is required as normal, and an observant foe might later spot a hidden halfling or elf: “I see you behind that guard, you tricksy halfling!”
Sage Advice Compendium v2.7 r2021
Mask of the Wild.
Do the lightfoot halfling and wood elf hiding racial traits allow them to hide while observed? The lightfoot halfling and wood elf traits—Naturally Stealthy and Mask of the Wild—do allow members of those subraces to try to hide in their special circumstances even when observers are nearby. Normally, you can’t hide from someone if you’re in full view. A lightfoot halfling, though, can try to vanish behind a creature that is at least one size larger, and a wood elf can try to hide simply by being in heavy rain, mist, falling snow, foliage, or similar natural phenomena. It’s as if nature itself cloaks a wood elf from prying eyes—even eyes staring right at the elf! Both subraces are capable of hiding in situations when most other creatures can’t, but neither subrace’s hiding attempt is assured of success; a Dexterity (Stealth) check is required as normal, and an observant foe might later spot a hidden halfling or elf: “I see you behind that guard, you tricksy halfling!”
Sage Advice Compendium v2.7 r2021
Maddening Darkness
Does all magical darkness block darkvision? Magical darkness blocks darkvision only if the rules text for a particular instance of darkness says it does. For example, the darkness spell specifies that it produces a magical darkness that obstructs darkvision. That obstruction is a feature of the spell, not of magical darkness in general.
Sage Advice Compendium, v2.7 r2021
Darkness
Does all magical darkness block darkvision? Magical darkness blocks darkvision only if the rules text for a particular instance of darkness says it does. For example, the darkness spell specifies that it produces a magical darkness that obstructs darkvision. That obstruction is a feature of the spell, not of magical darkness in general.
Sage Advice Compendium, v2.7 r2021
Darkness
Does all magical darkness block darkvision? Magical darkness blocks darkvision only if the rules text for a particular instance of darkness says it does. For example, the darkness spell specifies that it produces a magical darkness that obstructs darkvision. That obstruction is a feature of the spell, not of magical darkness in general.
Sage Advice Compendium, v2.7 r2021
Darkness
Does all magical darkness block darkvision? Magical darkness blocks darkvision only if the rules text for a particular instance of darkness says it does. For example, the darkness spell specifies that it produces a magical darkness that obstructs darkvision. That obstruction is a feature of the spell, not of magical darkness in general.
Sage Advice Compendium, v2.7 r2021
Maddening Darkness
Does all magical darkness block darkvision? Magical darkness blocks darkvision only if the rules text for a particular instance of darkness says it does. For example, the darkness spell specifies that it produces a magical darkness that obstructs darkvision. That obstruction is a feature of the spell, not of magical darkness in general.
Sage Advice Compendium, v2.7 r2021
Darkvision
Does all magical darkness block darkvision? Magical darkness blocks darkvision only if the rules text for a particular instance of darkness says it does. For example, the darkness spell specifies that it produces a magical darkness that obstructs darkvision. That obstruction is a feature of the spell, not of magical darkness in general.
Sage Advice Compendium, v2.7 r2021
Darkvision
Does all magical darkness block darkvision? Magical darkness blocks darkvision only if the rules text for a particular instance of darkness says it does. For example, the darkness spell specifies that it produces a magical darkness that obstructs darkvision. That obstruction is a feature of the spell, not of magical darkness in general.
Sage Advice Compendium, v2.7 r2021
Shifting
How long do a shifter’s temporary hit points last? Unless a feature says otherwise, temporary hit points last until you finish a long rest (PH, 198).
Sage Advice Compendium, v2.7 r2021
Shifting.
How long do a shifter’s temporary hit points last? Unless a feature says otherwise, temporary hit points last until you finish a long rest (PH, 198).
Sage Advice Compendium, v2.7 r2021
Draconic Bloodline
Can a dragonborn sorcerer with a draconic bloodline have two different kinds of Draconic Ancestry? A dragonborn sorcerer can choose a different ancestor for the racial trait and for the Dragon Ancestor feature. Your choice for the racial trait is your actual ancestor, while the choice for the class feature could be your ancestor figuratively— the type of dragon that bestowed magic upon you or your family or the kind of draconic artifact or location that filled you with magical energy.
Sage Advice Compendium v2.7 r2021
Draconic Bloodline
Can a dragonborn sorcerer with a draconic bloodline have two different kinds of Draconic Ancestry? A dragonborn sorcerer can choose a different ancestor for the racial trait and for the Dragon Ancestor feature. Your choice for the racial trait is your actual ancestor, while the choice for the class feature could be your ancestor figuratively— the type of dragon that bestowed magic upon you or your family or the kind of draconic artifact or location that filled you with magical energy.
Sage Advice Compendium v2.7 r2021
Draconic Ancestry
Can a dragonborn sorcerer with a draconic bloodline have two different kinds of Draconic Ancestry? A dragonborn sorcerer can choose a different ancestor for the racial trait and for the Dragon Ancestor feature. Your choice for the racial trait is your actual ancestor, while the choice for the class feature could be your ancestor figuratively— the type of dragon that bestowed magic upon you or your family or the kind of draconic artifact or location that filled you with magical energy.
Sage Advice Compendium v2.7 r2021
Naturally Stealthy
Do the lightfoot halfling and wood elf hiding racial traits allow them to hide while observed? The lightfoot halfling and wood elf traits—Naturally Stealthy and Mask of the Wild—do allow members of those subraces to try to hide in their special circumstances even when observers are nearby. Normally, you can’t hide from someone if you’re in full view. A lightfoot halfling, though, can try to vanish behind a creature that is at least one size larger, and a wood elf can try to hide simply by being in heavy rain, mist, falling snow, foliage, or similar natural phenomena. It’s as if nature itself cloaks a wood elf from prying eyes—even eyes staring right at the elf! Both subraces are capable of hiding in situations when most other creatures can’t, but neither subrace’s hiding attempt is assured of success; a Dexterity (Stealth) check is required as normal, and an observant foe might later spot a hidden halfling or elf: “I see you behind that guard, you tricksy halfling!”
Sage Advice Compendium v2.7 r2021
Mask of the Wild
Do the lightfoot halfling and wood elf hiding racial traits allow them to hide while observed? The lightfoot halfling and wood elf traits—Naturally Stealthy and Mask of the Wild—do allow members of those subraces to try to hide in their special circumstances even when observers are nearby. Normally, you can’t hide from someone if you’re in full view. A lightfoot halfling, though, can try to vanish behind a creature that is at least one size larger, and a wood elf can try to hide simply by being in heavy rain, mist, falling snow, foliage, or similar natural phenomena. It’s as if nature itself cloaks a wood elf from prying eyes—even eyes staring right at the elf! Both subraces are capable of hiding in situations when most other creatures can’t, but neither subrace’s hiding attempt is assured of success; a Dexterity (Stealth) check is required as normal, and an observant foe might later spot a hidden halfling or elf: “I see you behind that guard, you tricksy halfling!”
Sage Advice Compendium v2.7 r2021
Trance
Does the Trance trait allow an elf to finish a long rest in 4 hours? If an elf meditates during a long rest (as described in the Trance trait), the elf finishes the rest after only 4 hours. A meditating elf otherwise follows all the rules for a long rest; only the duration is changed.
Sage Advice Compendium v2.7 r2021
One must be able to add one's own private links to and from public information. One must also be able to annotate links, as well as nodes, privately.
Annotations were part of the earliest vision of the World Wide Web
Sau giai đoạn giảm sốc vừa qua, có nhiều NĐT đã bị thua lỗ nặng nề. Đây là điều đáng buồn, nhưng chúng ta nên bình tâm lại. Tôi xin chia sẻ một số quan điểm về giai đoạn hiện nay:1. Đáy thường chỉ nhận biết khi đã qua, nhưng chúng ta phải nhận ra, chứ không nên bảo thủ 1 chiều. Mốc 1157 là đáy 1, mốc 1203 có thể là đáy 2. Cả 2 mốc này đã được confirm.2. Thị trường đã bình tâm trở lại, không chỉ là vấn đề tâm lý, mà cả bao gồm cả về những vấn đề khác như trạng thái Margin, short sell, ...3. Thanh khoản giai đoạn tới sẽ không còn như trước vì lý do dòng tiền rút ra (một phần từ Trái phiếu DN, một phần tiền SX-KD, một phần do thua lỗ, một phần do quá sợ CK, ...). Nhưng nếu quan sát kỹ, chúng ta có thể nhận thấy mức 10k-11k tỷ / phiên là đáy thanh khoản. Kịch bản có xác suất cao trong thời gian tới là tăng dần đều, ổn định ở mức 14k-15k tỷ/ phiên.4. Vĩ mô thế giới có nhiều bất lợi, nhưng vĩ mô trong nước lại không quá bi quan. Gói hỗ trợ LS được tung ra kỳ này khá lớn, hy vọng có thể giữ ổn định TT về khía cạnh dòng tiền. Bên cạnh đó, giải ngân đầu tư công cũng kỳ vọng thúc đẩy tăng trưởng để GDP năm nay vẫn đạt mức 7%. Đặc biệt, dù siết chặt hơn nhưng vấn đề Trái phiếu DN, nợ xấu ngân hàng vẫn có chiều hướng "ném chuột không làm vỡ bình". Cho nên chỉ cần TT Mỹ đừng quá xấu (chẳng hạn DJ đừng thủng 30k), thì Vn-index hoàn toàn phù hợp với điểm 13xx. Nếu dòng tiền mạnh hơn (chẳng hạn lên lại 20k tỷ / phiên), thì rật có thể sẽ chinh phục được 14xx. Rõ ràng, nội tại vĩ mô được định giá lại ở mức 1200-1400 là phù hợp, chứ không còn câu chuyện 1600-1800 cho năm nay được, nhưng cũng rất khó xuyên thủng 1200.5. Với thị trường bình ổn hơn, sẽ không còn câu chuyện "ăn bằng lần" hay "lỗ sấp mặt" nữa. Sẽ có từng giai đoạn các nhóm ngành mạnh yếu khác nhau. Trong giai đoạn này tôi ưu tiên nhóm bank, vận tải biển, bán lẻ, năng lượng. Tôi sẽ bỏ qua các nhóm có tính chu kỳ như thép, phân bón, ck. Còn một số nhóm ngành tương đối khó hơn như bđs, kcn, thủy sản, hóa chất, tôi sẽ hành xử rất thận trọng.188Like
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Trong kỳ báo cáo gần nhất về chỉ số CPI của Mỹ đã có nhiều ý kiến cho rằng đã tạo đỉnh ở mốc 8.3%. Có nhiều khả năng khi báo cáo mới sẽ ra khoảng 10 ngày nữa sẽ nhìn thấy con số giảm rõ rệt. Dù ở VN luôn có độ trễ nhất định, nhưng khả năng CPI của VN cũng vẫn trong tầm kiểm soát, khoảng 5.0%-5.5% cho năm nay. Còn về Vn-Index thì rõ ràng sự điều chỉnh mạnh về đến dưới 1200 vì nhiều lý do khác nhau, nhưng có lẽ cũng đã phản ánh đầy đủ những gì xấu nhất. Việc TT có tăng trở lại hay không là khó dự báo, nhưng có thể tự tin rằng vùng 1200 đã confirm là đáy của Vn_index trong năm 2022. Còn sẽ tăng đến bao nhiêu, khi nào tăng, tăng ở dòng nào, lại là câu chuyện cần sự tư vấn chuyên sâu hơn.62Like
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Trong ngắn hạn mọi dự báo đều có xác suất sai rất cao, tuy nhiên cá nhân tôi vẫn duy trì các dự báo này dựa trên cơ sở lập luận của mình. Không hẳn để hô hào (vì chả để làm gì), để câu view vì tôi không cần điều đó, cái chính là vì quá đam mê và luôn sống trong nhịp đập của thị trường. Có nhiều người lôi các tus cũ của tôi đã từng ngợi khen VCI, TCB, HPG, ra để bêu riếu, hoặc giả lôi những nhận định lạc quan của tôi về Vn-Index lên 1600, 1800 trước đây để "dìm hàng". Thực lòng mà nói, tôi cũng rất đau lòng khi những nhận định của mình gây ra những điều chưa tốt cho mọi người, trong đó có cả bản thân tôi. Nhưng bên cạnh đó, tôi nghĩ rằng đó là cuộc sống, đó là sự vận hành của thị trường mà chúng ta luôn vui mừng học hỏi từ những thất bại. Tôi sẽ tiếp tục đồng hành, tiếp tục chia sẻ những tâm huyết của mình, đặc biệt chú trọng các vấn đề về học thuật, về phương pháp và các bài học từ kinh nghiệm của bản thân. Bạn nào thấy có ích có thể đọc và theo dõi, thấy vô bổ chỉ cần bỏ qua là xong, đừng bận lòng mà chửi bới vì chả có giá trị gì.149Like
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RRID:ZFIN_ZDB-GENO-190307-7
DOI: 10.7554/eLife.66118
Resource: (ZFIN Cat# ZDB-GENO-190307-7,RRID:ZFIN_ZDB-GENO-190307-7)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-GENO-190307-7
RRID:ZFIN_ZDB-GENO-191029-7
DOI: 10.7554/eLife.66118
Resource: (ZFIN Cat# ZDB-GENO-191029-7,RRID:ZFIN_ZDB-GENO-191029-7)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-GENO-191029-7
En somme, les études sur la communication des élèves atteints d’autisme permettent de mettre en évidence l’importance d’un contexte riche en stimulations appropriées (sons et images), mais également une évidente « stabilité » de l’information à décoder, le suivi des émotions des personnages, le rôle de l’imitation dans les apprentissages. Ces résultats encouragent donc l’usage d’outils informatiques adéquats pour améliorer la communication sociale chez les enfants atteints d’autisme.
L'association de deux sujets qui n'ont pas de corrélation vérifiéé, revient dans la conclusion en contradiction avec la conclusion de l'étude de Ramdoss, S et al.
Nous allons montrer par une courte analyse de quelques études l’impact du travail éducatif informatisé dans l’apprentissage de la communication sociale chez des enfants atteints d’autisme.
En contradiction avec l'hypothèse :
Results suggest that CBI should not yet be considered a researched-based approach to teaching communication skills to individuals with ASD. However, CBI does seem a promising practice that warrants future research. Les résultats suggèrent que le CBI ne devrait pas encore être considéré comme un approche fondée sur la recherche pour enseigner les compétences en communication aux personnes ayant Troubles du Spectre Autistique. Cependant, le CBI semble être une pratique prometteuse qui justifie des recherches futures.
In the president's favor is the fact that his order commands the steel industry to follow policy set by Congress, not the president himself. The lawmaking power in the United States is within Congress's domain, but Congress has clearly chosen to rid itself of that power and give it to the president.
This whole statement is false.
Looking at Johnson's actions, there is no evidence of an expressive element in his actions
incorrect- there IS an expressive element in Johnson's actions, however, the we're looking at whether the expression actions are likely to 'disturb the peace' or whether it falls in the conduct of 'fighting words'
El factor tiempo de trabajo está también muy relacionado con la variabilidad de la presión de inyección, ya que a medida que incrementa el tiempo de operación de un motor, se incrementa el desgaste de las piezas, siendo agravado el sistema de alimentación por la variación de la calidad del combustible
También depende del factor de cuanto uso se le ha dado a ese motor, no solo por el incremento de desgaste significa que va a bajar su calidad.
The Baltimore school district faced a crisis
Wrong, Cleveland school district faced a crisis
7–2
The Court ruled by a vote of 5-4.
5 percent of students used their vouchers at private schools.
Incorrect - 97% of students used their vouchers at private schools
7–2
5-4
dichotomous
1: dividing into two parts
2: relating to, involving, or proceeding from dichotomy the plant's dichotomous branching a dichotomous approach can't be split into dichotomous categories
Yet most companies neglect design as a strategy tool. What they don’t realize is that design can enhance products, environments, communi-cations, and corporate identity.”
This is true because a Designer can communicate one's ideas in a clear and sensible way. Companies need design in order to get as many people to associate themselves with them.
maelstrom
1: a powerful often violent whirlpool sucking in objects within a given radius tried to shoot the canoe across a stretch of treacherous maelstrom — Harper's
2: something resembling a maelstrom in turbulence the maelstrom enveloping the country a maelstrom of emotions
dissenting
missing info: Justices's Reed and Minton also joined in the dissent
dissenting
Concurring.
ZFIN: ZDB-ALT-160901-7
DOI: 10.1016/j.celrep.2021.109775
Resource: (ZFIN Cat# ZDB-ALT-160901-7,RRID:ZFIN_ZDB-ALT-160901-7)
Curator: @Naa003
SciCrunch record: RRID:ZFIN_ZDB-ALT-160901-7
ZDB-ALT-110309-7
DOI: 10.1016/j.devcel.2021.07.021
Resource: (ZFIN Cat# ZDB-ALT-110309-7,RRID:ZFIN_ZDB-ALT-110309-7)
Curator: @Naa003
SciCrunch record: RRID:ZFIN_ZDB-ALT-110309-7
RRID:ZFIN_ZDB-GENO-070702-7
DOI: 10.7554/eLife.44431
Resource: (ZFIN Cat# ZDB-GENO-070702-7,RRID:ZFIN_ZDB-GENO-070702-7)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-GENO-070702-7
RRID:ZFIN_ZDB-GENO-960809-7
DOI: 10.1111/bph.14560
Resource: ZFIN_ZDB-GENO-960809-7
Curator: @ethanbadger
SciCrunch record: RRID:ZFIN_ZDB-GENO-960809-7
RRID:ZDB-ALT-180515-7
DOI: 10.7554/eLife.54894
Resource: (ZFIN Cat# ZDB-ALT-180515-7,RRID:ZFIN_ZDB-ALT-180515-7)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-ALT-180515-7
EducationSavings and investmentScientific researchHealthcareInternational tradeInfrastructureSpecial economic zones
This is a combo of Giacomo's list and OS's list. I've devoted one explanation card to each item on this list. Education, savings/investment, and scientific research are featured by both Giacomo & OS; we may want to cut some of the others
Results for individual PALB2 variants were normalized relative to WT-PALB2 and the p.Tyr551ter (p.Y551X) truncating variant on a 1:5 scale with the fold change in GFP-positive cells for WT set at 5.0 and fold change GFP-positive cells for p.Y551X set at 1.0. The p.L24S (c.71T>C), p.L35P (c.104T>C), p.I944N (c.2831T>A), and p.L1070P (c.3209T>C) variants and all protein-truncating frame-shift and deletion variants tested were deficient in HDR activity, with normalized fold change <2.0 (approximately 40% activity) (Fig. 1a).
AssayResult: 4.1
AssayResultAssertion: Indeterminate
StandardErrorMean: 0.56
A total of 84 PALB2 patient-derived missense variants reported in ClinVar, COSMIC, and the PALB2 LOVD database were selected
HGVS: NM_024675.3:c.110G>A p.(Arg37His)
SUPPLEMENTARY DATA
AssayResult: 92.03
AssayResultAssertion: Not reported
PValue: > 0.9999
Comment: Exact values reported in Table S3.
To this end, 44 missense variants found in breast cancer patients were identified in the ClinVar database (https://www.ncbi.nlm.nih.gov/clinvar) and/or selected by literature curation based on their frequency of description or amino acid substitution position in the protein (Supplemental Table S1).
HGVS: NM_024675.3:c.232G>A p.(Val78Ile)
Source Data
AssayResult: 89.72
AssayResultAssertion: Not reported
ReplicateCount: 2
StandardErrorMean: 7.95
Comment: Exact values reported in “Source Data” file.
Source Data
AssayResult: 94.84
AssayResultAssertion: Not reported
ReplicateCount: 2
StandardErrorMean: 20.56
Comment: Exact values reported in “Source Data” file.
Source Data
AssayResult: 105.41
AssayResultAssertion: Not reported
ReplicateCount: 2
StandardDeviation: 9.45
StandardErrorMean: 6.68
Comment: Exact values reported in “Source Data” file.
We, therefore, analyzed the effect of 48 PALB2 VUS (Fig. 2a, blue) and one synthetic missense variant (p.A1025R) (Fig. 2a, purple)29 on PALB2 function in HR.
HGVS: NM_024675.3:c.124G>A p.(E42K)
Most Suspected Brugada Syndrome Variants Had (Partial) Loss of Function
AssayResult: 3.4
AssayResultAssertion: Abnormal
ReplicateCount: 22
StandardErrorMean: 0.8
Comment: This variant had loss of function of peak current (<10% of wildtype), therefore it was considered abnormal (in vitro features consistent with Brugada Syndrome Type 1). (Personal communication: A. Glazer)
we selected 73 previously unstudied variants: 63 suspected Brugada syndrome variants and 10 suspected benign variants
HGVS: NM_198056.2:c.1106T>A p.(Met369Lys)
RRID:ZDB-ALT-170927-7
DOI: 10.7554/eLife.54491
Resource: (ZFIN Cat# ZDB-ALT-170927-7,RRID:ZFIN_ZDB-ALT-170927-7)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-ALT-170927-7
RRID:ZDB-ALT-110215-7
DOI: 10.7554/eLife.54491
Resource: (ZFIN Cat# ZDB-ALT-110215-7,RRID:ZFIN_ZDB-ALT-110215-7)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-ALT-110215-7
RRID:ZFIN_ZDB-ALT-120221-7
DOI: 10.7554/eLife.53403
Resource: (ZFIN Cat# ZDB-ALT-120221-7,RRID:ZFIN_ZDB-ALT-120221-7)
Curator: @scibot
SciCrunch record: RRID:ZFIN_ZDB-ALT-120221-7
Supplemental material
AssayResult: 101, 106
AssayResultAssertion: Normal
Comment: See Table S3 for details
Supplemental material
AssayResult: 4, 5
AssayResultAssertion: Abnormal
Comment: See Table S3 for details
We analysed a total of 82 blood samples derived from 77 individuals (online supplemental table 3). These 77 individuals corresponded either to new index cases suspected to harbour a pathogenic TP53 variant or to relatives of index cases harbouring TP53 variants.
HGVS: NM_000546.5:c.1054G>T p.(Asp352Tyr)
RRID:ZFIN_ZDB-GENO-190822-7
DOI: 10.1002/dneu.22791
Resource: (ZFIN Cat# ZDB-GENO-190822-7,RRID:ZFIN_ZDB-GENO-190822-7)
Curator: @Naa003
SciCrunch record: RRID:ZFIN_ZDB-GENO-190822-7
Full Card Number
I apologize for any inconvenience this may cause you; however, your PIN must be verified in the automated system before I am able to share account specific information for your security.
RRID:ZFIN_ZDB-GENO-200128-7
DOI: 10.7554/eLife.60445
Resource: (ZFIN Cat# ZDB-GENO-200128-7,RRID:ZFIN_ZDB-GENO-200128-7)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-GENO-200128-7
'[My] mouth [that] cursed you shall bless [you] as well!
being rude and kind in the same time.
Come, Shamhat, 1 will fix your destiny,
It shows the confident.
In [your presence] I will pray [to Anu,] father of the gods,
It shows that Sumerians believe in different gods.
He has a second dream, in which he is dragged down to the Netherworld by the Angel of Death and granted a vision of hell.
It shows that Sumerians believe to the life after death.
RRID:ZFIN_ZDB-GENO-070702-7
DOI: 10.7554/eLife.44431
Resource: (ZFIN Cat# ZDB-GENO-070702-7,RRID:ZFIN_ZDB-GENO-070702-7)
Curator: @evieth
SciCrunch record: RRID:ZFIN_ZDB-GENO-070702-7
RRID:ZFIN_ZDB-GENO-160122-7
DOI: 10.1016/j.cell.2019.01.019
Resource: (ZFIN Cat# ZDB-GENO-160122-7,RRID:ZFIN_ZDB-GENO-160122-7)
Curator: @ethanbadger
SciCrunch record: RRID:ZFIN_ZDB-GENO-160122-7
allele id lookup result: https://reg.clinicalgenome.org/redmine/projects/registry/genboree_registry/by_caid?caid=CA915940549
7
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000717
muscular hypotonia
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001252
intellectual disability
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001249
motor development
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001270
speech and language development
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0000750
developmental delay
Monarch lookup result: https://monarchinitiative.org/phenotype/HP:0001263
The only place he could think of was the Trianon in mid-town
Mapping Assignment Location - Emi and Ichiro go dancing
Thinking that he heard a knocking on the front door, he remained still and listened. It came again, faintly, hesitantly. He went through the store, wondering who it could be.
Emi coming to Ichiro's store to talk about his mother's passing - Mapping Assignment location
He squirmed uneasily and wondered if Taro would acknowledge the telegram which he had sent the day before after finally having hunted down the information that he was taking basic training in a California camp
Mapping Assignment Location - Taro's location during Ma's funeral
HE FUNERAL WAS HELD SEVERAL DAYS LATER AT THE BUDDHIST church up on the hill next to a playground.
Mapping Assignment Location - Ma's funeral
They did not speak again until the car was beside the grocery store.
Mapping assignment
I went to Portland with him.”
Mapping Assignment Location
‘Put my ashes in an orange crate and dump them in the Sound off Connecticut Street Dock where the sewer runs out,’
Mapping Assignment Location
It scared him to think that he might be sobering up.
What scared him more, sobering up or facing reality?
We can take any data set and put it into a pie chart, a continuous graph, a scatter plot, a tree map and so on.
I have always questioned this because I feel that some information or data can not be put into any sort of visual representation.
The use of a legend and symbols helps, but cartographers, artists, and designers have also introduced spatial distortions and warps that are unusual and imaginative.
I never thought that mapping would be so complex!
how we might expand the conventions of map-making to include the kinds of experiential aspects of human culture that are absent from many conventions.
I am interested to see where the mapping industry goes, as everything is becoming digitalized, will paper maps still be used? What demographic still buys them now and who will buy them in the future?
very projection is a distortion, but the nature of the distortions varies depending on the ways the images are constructed and the purpose they are meant to serve
I find this to be so interesting. Every map of the globe that I look at I assumed was the same, but this is making me rethink that logic.
Innovation in Customers' Hands at New 7-Eleven® Lab Store Retailer Celebrates New Sylvan | Thirty Location with March 22 Grand Opening News provided by 7-Eleven, Inc. Mar 27, 2019, 09:30 ET Share this article IRVING, Texas, March 27, 2019 /PRNewswire/ -- Made-to-order smoothies and agua frescas … street tacos on handmade tortillas … a growler refill station pouring local craft beers … baked-in-store cookies and croissants … patio and inside dining areas … The newest 7-Eleven® location is a lab store and an experiential testing ground, where customers can try and buy the retailer's latest innovations in a revolutionary new store format. The newest 7-Eleven location in Dallas is a lab store and an experiential testing ground, where customers can try and buy the retailer’s latest innovations in a revolutionary new store format. 7-Eleven, Inc. celebrated the grand opening of its new lab store in Dallas – and the only one in the U.S. – March 22. The store is located at the Sylvan | Thirty retail and restaurant development on Sylvan Avenue, north of Interstate 30. This location is less than two miles from the original Southland Ice House in Oak Cliff where 7-Eleven pioneered the convenience retailing concept more than 90 years ago. "Convenience retailing is light years away from the days of bread and milk being sold from ice docks in 1927, and the industry is changing at a faster rate than ever before," said Chris Tanco, 7-Eleven executive vice president and chief operating officer. "7-Eleven stays at the forefront by pushing the boundaries and being unafraid to try new things. This new lab store will serve as a place to test, learn and iterate new platforms and products to see what really resonates with customers and how we can use those learnings to influence future store designs."The lab store is also the first 7-Eleven location to incorporate the Laredo Taco Company® taqueria, and the first Laredo Taco Company location in Dallas. 7-Eleven acquired the taqueria along with Stripes® convenience stores in South Texas as part of the 1,000-store acquisition from Sunoco in 2018. Laredo Taco Company is famous in South Texas for its handmade tortillas made from scratch in stores every day as well as its popular salsa bar with on-site, daily prepared salsas, guacamole and pico de gallo. Tacos, quesadillas and plate meals include specialties not always seen in quick-serve Tex-Mex restaurants such as carne guisada, barbacoa, picadillo bistec, carnitas and breakfast tacos made with hand-cracked eggs.Some of the other innovative ideas customers will see at the new 7-Eleven lab store include: Made-to-order coffee drinks, cold-pressed juices, smoothies and agua frescas that give customers the option to customize their drinks in a full-service beverage format. Additionally, it carries novelty beverages on tap such as nitro cold brew, kombucha and organic teas. "The Cellar," an alcove dedicated to an expanded selection of wines and craft beers, with a nearby growler station that features a rotating selection of local craft beer, cider and ales on tap. At the growler station, customers can enjoy a draft of their favorite beverage with their meal onsite or fill a growler to take home. A cold treats bar with frozen yogurt, ice cream and multiple toppings Cookies, croissants and more baked-in-store daily Digital initiatives that enhance the shopping experience. Scan & Pay technology that allows customers to skip the checkout line and pay for their (non-age-restricted) purchases on their smartphones. Indoor and patio restaurant-style seating in the Laredo Taco Company portion of the store as well as bar-seating across the front windows in the retail space. Many of the new items in this 7-Eleven "innovation station" are limited-time offerings. "A lot has changed in retail and continues to change rapidly, especially the shopping experience," Tanco said. "This lab store is customer-focused and will explore new ideas that weren't even on the retail radar a few months ago."The new 7-Eleven lab store is also providing local jobs, and the company is looking for outgoing, customer-service-oriented employees for this innovative new retail-restaurant concept. To join the Sylvan | Thirty location as a sales or restaurant associate, interested people can apply online at: https://careers-7-eleven.icims.com. Under "Start your job search here," input 54716 for sales associate or 54817 for restaurant associate.About 7-Eleven, Inc.7-Eleven, Inc. is the premier name and largest chain in the convenience-retailing industry. Based in Irving, Texas, 7-Eleven operates, franchises and/or licenses more than 67,000 stores in 17 countries, including 11,800 in North America. Known for its iconic brands such as Slurpee®, Big Bite® and Big Gulp®, 7-Eleven has expanded into high-quality salads, side dishes, cut fruit and protein boxes, as well as pizza, chicken wings, cheeseburgers and hot chicken sandwiches. 7-Eleven offers customers industry-leading private brand products under the 7-Select® brand including healthy options, decadent treats and everyday favorites, at an outstanding value. Customers also count on 7-Eleven for bill payments, self-service lockers and other convenient services. Find out more online at www.7-Eleven.com, via the 7Rewards® customer loyalty platform on the 7-Eleven mobile app, or on social media at Facebook, Twitter and Instagram.SOURCE 7-Eleven, Inc.
Oh Thank Heaven for 7-Eleven!®A success story fueled by customers’ needs “Give the customers what they want, when and where they want it.” Joe C. Thompson Jr. | 7‑Eleven Founder The 7‑Eleven brand is known and loved around the world, and our iconic products are a big part of the American culture. And although we’ve grown significantly over the years, our focus stays fixed on making life easier for customers. This simple idea is the reason we’re the marketplace leader. It’s also why our customers, employees, Franchisees and community leaders are proud to be part of the 7‑Eleven story. Nonstop Innovation & Customer Obsession We Did 7‑Eleven has a legacy of innovation. We were the first to provide to-go coffee cups, offer a self-serve soda fountain, operate for 24 hours a day, and yes, we even coined the phrase “BrainFreeze®” in honor of the world’s favorite frozen drink. Then came the innovation of some of our most popular menu items: the SLURPEE® drink, the BIG GULP® and then the BIG BITE®. Now, we continue our history of innovation and power it through digital initiatives. We Will From our humble beginning as the world’s first convenience store, 7‑Eleven continues its pursuit of innovative ways to cater to a new, digital-savvy generation of shoppers. As technology redefines how customers shop, we make sure to remain two steps ahead. At 7‑Eleven, we are customer-obsessed. We always poll customers to ensure we are bringing them solutions that they can’t even imagine. From offering convenient and user-friendly technology that earns our customers free products to having an ice-cold SLURPEE® drink delivered straight to their door, we are the leader in convenience and we constantly put our customers at the center of design and development. We Are It’s no secret: customers are starved for time as our world becomes increasingly connected and on the go. Our digital team strives to be at the forefront of innovation, providing effortless and convenient solutions for our customers’ needs before they even know to ask for them. We are committed to developing experiences of the future by bringing our stores to our customers wherever they are and whenever they need us. We are continuing our long-standing legacy of innovation through software and technology enhancements. We are transforming our business, one digital initiative at a time. We are redefining convenience. Learn More About Our Apps The best kind of neighbor Convenience may be our focus, but serving is our business. And that business extends beyond our stores into the communities where our customers, employees and Franchisees live, work and play. Being a great neighbor is all about investing and getting involved. It’s also about responsibility, which is one of our key business principals. That’s why we put such a focus on serving people, improving our products and protecting the planet. 7‑Eleven is proud to set the standard for responsible retailing in the convenience industry. Learn more Our Culture To lead, we serve 7‑Eleven has always been about serving the needs of our customers. This philosophy has also extended to our 7‑Eleven team, which is why our Leadership Principles play such a big part in our organization. To lead, we serve. It’s a big idea and it makes a tremendous difference in the lives of our customers, our Franchisees and our licensees. All kinds of people with one focus: making life easier We’re the convenience leader for a reason – and it has everything to do with our diverse and multi-talented team of men and women who are passionate about making life convenient. For decades – nine to be exact – we’ve counted on their unique perspectives to help us innovate and grow. They are the reasons we continue to lead in the industry. Room to move around and grow Cross-functional learning is not just allowed at 7‑Eleven, it’s applauded. We’ve got the size, stability and resources that make it possible for employees to find rewarding careers. People in all facets of our global organization discover opportunities with room to move around, try on new roles and discover untapped talents. We know that when people love what they do, everyone wins. A top-five franchisor 7‑Eleven is a brand that’s recognized worldwide. We’ve also made a reputable name for ourselves in the franchise business, and are consistently ranked as a top-five franchisor. A turnkey business model, world-class training, ongoing corporate support and special financing programs are available to increase the success rates of our Franchisees. The Store Support Center: the engine behind our stores If our stores are the fuel for busy communities, then our Store Support Center is the fuel for our stores. This is where the innovating, strategizing, forecasting, training and troubleshooting take place. From operations management and logistics to real estate, IT and human resources, our Store Support Center teams work hard to further our mission of convenience, not only for customers, but also for our Franchisees, employees and the communities we serve.
https://corp.7-eleven.com/corp/about#annotations:gEbOcPm6Eemv2Bv2QdZKbA
7-Eleven moves to support animal welfare By Nick Hall | 17 Feb 2019 View comments Global convenience chain, 7-Eleven has made major changes to its supplier sourcing agreement, eliminating caged eggs for the first time. The move follows ongoing criticism of the Australian cage egg farming industry, with several brands and chains making the early decision to move to wholly sustainable sourcing in-line with welfare standards. Working closely with suppliers across all states, 7-Eleven has now ensured that only free range eggs would be available for order by all stores. Clayton Ford, General Manager Corporate Affairs, 7-Eleven said the decision to phase out cage eggs was developed in accordance with franchisee wishes, with the convenience giant announcing it will increase support to assist in the transition. “Whilst our franchisees are free to engage with alternative suppliers due to our franchise agreement, we will continue to work alongside them to encourage their involvement in this initiative by sourcing free range eggs via our centralised supply chain,” Ford said. “We recognise that this is just one step, and we will continue to review animal welfare commitments in our supply chain and 7-Eleven branded products as opportunities arise.” Want to launch a convenience store of your own? Take a look at all available franchising opportunities here.
Accuracy (recovery
Iron Curtain
How effective was the "Iron Curtain"?
Romero was assassinated in 1980
His views on social and political issues was not taken well and eventually got his assassinated.
In the event of armed attack in Europe on one or more of the Parties to the Treaty by any state or group of states, each of the Parties to the Treaty, in the exercise of its right to individual or collective self-defence in accordance with Article 51
A "attack one, attack all" mentality
Oral administration of doses
ROS assay
Cytotoxicity assay by Sulphorhodamine B (SRB) me
Cytotoxicity assay by MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyltetrazolium Bromide)method
Cytotoxicityscreenin
Test for cardiac glycosides
Test for Terpenoids
Tests for Flavonoids
Tests for Phenolic compounds
Tests for Saponins
Tests for Tannins
Tests for Alkaloids
Test for Sterols
Qualitative study of phytochemicals of N. sativadurin
PCR amplification
Harvesting of cultures
Effect of pHon growth and sporulation of the bioagent
Effect of temperature on growth of bioagent
Effect of different media on growth of bioagentTrichoderma
Cultural characteristicsof bioagent Trichoderma
Relative distribution of virulence genes among V. parahaemolyticusfrom Cochin estuary, shrimp farm and sea food
Production of phosphatase
PCR amplification of 16S rRNA gene
DNA isolatio
Molecular confirmation of Vibrioby 16S rRNA gene se
Growth at different temperatures
Growth at different temperature
MAR indexingand antibiotic resistance pattern amon
Digestion of Plasmid DNA
Minipreparation of plasmid DNA from transformed co
Screening of recombinant E. coli clone
Transformation of ligation mixture in electro-competent E. coli host cells (DH5ααααstrain)
Transformation of ligated product in chemically competent E. coli host cells (DH5αααα strain)
Ligation reactions
Dephosphorylation of vector
Restriction digestion
Ligation of eluted PCR product in pBSK vector
Cloning of gel eluted PCR produc
Statistical analysis
PubChem Compound Database Screening
Assessmentof mitochondrial membrane potential(MMP or ΔΨm)
Growth kinetic studies
Kirby-Bauer Disk diffusion method
Antimicrobial activity
. Statistical analysis
Lactic dehydrogenase
Malic dehydrogenase
Succinic dehydrogenase
Estimation of dehydrogenases activities (SDH, MDHand LDH) in muscle and gill tissue of fish, Labeo rohita
Biological Yield(g)
Concentration of ideal carbon source
Post-vaccination declination of Transitional B cell population and increase in memory B cells in HBV positive newborns
Cell Counting
Semi dry transfer buffer
Stripping of Ni-NTA agarose beads
Cell transfection
GFP-β-cateninC-C (423-634 a.a) (ARM-C)
Chemicals/inhibitors
Sonication of bacteria
Cytotoxicity assay
ELISA dilution buffer
Lymphokines Assay
Gene cloning
Large scale plasmid extraction
Separation ofCurcuminoids
Estimation of Stevioside
Extraction from the plant material
Extraction from the plant material
Extraction from the plant material
Qmmtification of stevioside
Mesomorphic endomor~:
Humerus
The semisynthetic a globin was purified from the mixture by CM52 urea chromatography as explained below. The lyophilized sample was dissolved in 0.005 M phosphate buffer (pH 6.9) containing 8 M urea and 0.05 M 2-mercaptoethanol at a concentration of I 0-15 mg/ml and loaded onto a CM52 column (16 em x 1.5 em) equilibrated with the same buffer. After an initial wash with the same buffer, two linear gradients of(a) 100 ml each of0.005 M to 0.03 M and (b) 100 ml each of0.02 M to 0.05 M phosphate buffer (all buffers contained 8 M urea and 0.05 M 2-mercaptoethanol, and were adjusted to pH 6.9) were employed at a flow rate of 45 ml/h to elute the semisynthetic a globin. The column was finally washed with 0.05 M buffer to elute unreacted a31-141 fragment from the column. The elution profile was monitored at 280 nm. The fractions for semi-synthetic a globin were pooled, extensively dialyzed against 0.1% TF A and lyophilized. The semisynthetic yield of the protein varied between 35% to 45%