In the layered architecture pattern, each layer fulfills a specific responsibility and role within the application. Some focus on user interface logic, while others handle the execution of business rules. These layers complement each other's unique purposes, but they aren't explicitly reliant on one another to perform their own tasks.

In software architecture, layers act as individual processes within the infrastructure of an application. These layers typically form a pattern, also called the n-tier architecture pattern.

No longer a free trial but free forever

We also know people need a good sized group and time to see the impact and value of a platform like Stack Overflow for Teams. Our previous 30 day free trial of our Basic tier wasn’t long enough. Now, Stack Overflow for Teams has a free tier for up to 50 users, forever.

tier 1 contains all changes in the amino acid coding regions of annotated exons, consensus splice-site regions, and RNA genes (including microRNA genes). Tier 2 contains changes in highly conserved regions of the genome or regions that have regulatory potential. Tier 3 contains mutations in the nonrepetitive part of the genome that does not meet tier 2 criteria, and tier 4 contains mutations in the remainder of the genome