- Nov 2024
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www.youtube.com www.youtube.com
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the only way you can visualize the vaces because they're too tiny to be visualized by standard microscopy labeling with florescent dyes is about the only way we can um easily identify what molecules have been passed down from the vesicles to The Germ cells but that's very restrictive you see because there will be millions of different molecules in a single visle to be faced with only being able to label three or four of those otherwise we can't make out the the differences is it's very tedious
for - evolution - work to identify non-DNA information passed down to germ line - millions of permutations - fluorescence technique applied to only a few at a time - tedious work - Denis Noble
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- Jan 2024
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Fluorescence Activated Cell Sorting (FACS). From sorting cells for single cell genomics to identifying the host range of a plasmid; from detecting metabolically active cells in an environmental sample to studying colitogenic-driving intestinal bacteria, FACS can be used in a multitude of applications to study microbial communities (Rinke et al. 2014, Klumper et al. 2015, Kalyuzhnaya et al. 2008, Hatzenpichler et al. 2016, Palm et al. 2014).
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- Sep 2021
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Local file Local file
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photobleaching
Photobleaching is the chemical alteration of the indicator dye, be it a fluorophore or a colorimetric dye, so that it is unable to fluoresce due to the destruction of covalent or non-covalent bonds due to non-specific binding caused by excitation light.
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- Nov 2020
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pubs.acs.org pubs.acs.org
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fluorescein isothiocyanate (Sigma-Aldrich, CAS: 3326-32-7) dissolved in PBS
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calibrants for red fluorescence, such as TexasRed, are currently being investigated.
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- Aug 2020
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www.biorxiv.org www.biorxiv.org
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Chen, Y., Yang, W.-H., Huang, L.-M., Wang, Y.-C., Yang, C.-S., Liu, Y.-L., Hou, M.-H., Tsai, C.-L., Chou, Y.-Z., Huang, B.-Y., Hung, C.-F., Hung, Y.-L., Chen, J.-S., Chiang, Y.-P., Cho, D.-Y., Jeng, L.-B., Tsai, C.-H., & Hung, M.-C. (2020). Inhibition of Severe Acute Respiratory Syndrome Coronavirus 2 main protease by tafenoquine in vitro. BioRxiv, 2020.08.14.250258. https://doi.org/10.1101/2020.08.14.250258
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