8,709 Matching Annotations
  1. Jul 2019
  2. sg.inflibnet.ac.in sg.inflibnet.ac.in
    1. Reaction resuspension
    2. Post-reaction clean up
    3. Sequencing PCR
    4. Sequencing reaction
    5. Digestion of Plasmid DNA
    6. Minipreparation of plasmid DNA from transformed co
    7. Screening of recombinant E. coli clone
    8. Transformation of ligation mixture in electro-competent E. coli host cells (DH5ααααstrain)
    9. Transformation of ligated product in chemically competent E. coli host cells (DH5αααα strain)
    10. Ligation reactions
    11. Dephosphorylation of vector
    12. Restriction digestion
    13. Ligation of eluted PCR product in pBSK vector
    14. Cloning of gel eluted PCR produc
    15. Gel elution of PCR Produ
    16. Scoring of amplification data points and construction of a den
    17. Analysis of PCR amplicons using agarose gel electrophoresis
    18. Cycling condition
    19. PCR reaction set up
    20. Basic requirements for PC
    21. PCR amplification of Dof domain and Dof genes from different
    22. Primer designing for PCR amplification of Dof domain and D
    23. Qualitative analysis of DNA by agarose gel electrophoresi
    24. Spectrophotometric quantification of genomic DNA
    25. DNA purification
    26. DNA extraction procedure
    27. Isolation of genomic DNA by CTAB method
    28. Glasswares, plasticwares and equipments
    1. The table 6.1 gives the mixing probabilities and the associated parametricvalues fork(number of components) = 2,3, and 4. It may be noted thatthe Log likelihood value is smaller fork= 4 (the results fork= 5 , 6 etc.are not better than that fork= 4 and hence are not given here). The fourcomponents Poisson Mixture model is given in table 6.2. It may be notedthat 58% of wards may have higher incidence/relative risk and the remainingwards have lesser/lower incidence for the Cancer disease. We computed theposterior probability for each component for each ward (see table 6.3). Eachward is assigned to a particular component so that the posterior probability islarger. These results are also given in table 6.3 Finally we present Choroplethmaps based on those results
    2. The Posterior Probability of Mixing Dis-tribution
    3. Algorithm
    4. EM Procedure
    5. Poisson Mixture
    6. Data Sources
    7. Poisson Mixtures Distribution
  3. Jun 2019
    1. Soil pH and electrical conductivity (EC)
    2. Experiment 2: Assessing the impacts of elevated temperature and N levels on yield and nutrient uptake in rice
    3. Experiment 1: Assessing the impacts of elevated CO2and N levels on yield and nutrient uptake in rice
    4. Experimental Design and Treatments
    5. Temperature Gradient Tunnels (TGT)
    1. Vaccination improved the Chemokine receptor CCR1, CCR3, CCR9 and Toll like receptor TLR2, TLR4 and TLR9 expression in HBsAgpositive newborns compared to healthy newborns.
    2. IFN γ production by CD8 T cells upon stimulation with PMA and viral peptides
    3. Pre-vaccination:Lower Chemokine and Toll like receptor expression in HBsAgpositive newborns:
    4. T cell phenotypic distribution in HBsAgPositive, HBsAgNegative from HBsAg positive mothers and healthy newborns.
    1. Chapter 1 of the text described how Eurasian peoples who were descendants of Africans, expanded and spread throughout the world influenced by environmental climate cycles and natural resources. Chapter 2 is about these groups who are now culturally and genetically distinctive after twelve thousand years of separation coming back into contact with each other and some of the disastrous results as a result. Columbas’s fleet was not the first contact between Europeans and Native Americans; it is likely that the Vikings were. The Vikings established colonies and interacted with some of the indigenous tribes of Canida as early as 1000 CE, almost 2500 years before Columbus. However, these Viking colonies did not last. It is likely the environment was a key factor in their failure. Beginning the 14th century, there was a mini ice age which occurred and lasted for four hundred years. It appears that increased cold temperatures made resupply of these colonies impossible, and it is also likely that food production was negatively impacted

      When Columbas was approved by the Spanish crown to sail, Europe was thriving economically and its population was growing due to the large expansion and productivity of fishing. The text estimates that the global population was approximately 500 million “evenly split between Europe, Africa, and the Americas” While Columbus did not discover an empty continent, in a few hundred years, the virgin soil epidemics would make that a reality in some regions of the Americas.

      It was the environment that forced Columbus to dock (permanently) with America when the Santa Maria “ran aground” on Christmas Day December 25th in 1492. After meeting with the local tribe, Columbus returned with two ships and some natural resources, including gold, wildlife, and crops, and wrote “Letter on the First Voyage” exaggerating some of his findings so as to receive approval to make another trip. In addition, Columbus transported native plants and animals of Central America back to Europe.

      As travel to the Americas increased, Europeans brought over many plants and animals that affected the American environment. New crops and livestock were introduced, and horses were also brought over which greatly changed the culture of the Great Plains Indians. As previously stated, Europeans also brought over very significant trait: their germs. It is likely that up to 90% of the American Indian population in 250 years died as a result of these germs. The reason that the conquistadors were successful in conquering some of the American Indian Civilizations was that the disease had already decimated their civilizations and societies and prevented the indigenous populations from effectively fighting back. Europeans owed much of their inherited immunity from these viruses to their contact with their livestock, which was not present in the Native American tribes. Some large native cities experienced such large population die-offs, that new European cities were built right on top of the old ones (Mexico City) because very few inhabitants were left

      Chapter 2 provides more information about how the environment altered the course of history for indigenous Americans and the European explorers. It is clear that with every significant historical event or trend, the environment had a causal role- impacting the decisions and ultimately histories of those involved.

    1. Overnight grown primary culture of E. coli cells (1 % v/v final concentration) was inoculated into 1 litre of LB media containing antibiotics. Culture was incubated at 37 oc at 200 rpm. Growth was monitored by measuring absorbance of E. coli broth at 600 nm. Culture was induced by adding 1 mM IPTG at an OD of 0.6 and was harvested after 4 hrs of induction. Samples were taken on an hourly basis after induction to check the kinetics of protein expression. Un-induced and induced E. coli cells were analyzed by SDS-PAGE to check the expression of recombinant protein.
    2. Growth and expression of recombinant proteins in E. coli cells
    1. The [3-PMB and a-PMB chains were eluted with a linear gradient of 500 ml each of 0.01 M potassium phosphate buffer (pH 6.5) and 0.015 M potassium phosphate buffer (pH 8.5) at a flow rate of 50 ml/hour. The chains were separately concentrated using Centriprep concentrators (Amicon) and stored in liquid nitrogen till further use
    2. The heme bound a and ~ subunits were obtained as described by Bucci (1981 ). Briefly, hemoglobin was reacted with PMB in an eight fold molar excess (8 moles of PMB per mole of hemoglobin). The reaction mixture was dialyzed extensively against 0.01 M potassium phosphate buffer (pH 6.5) and then loaded onto a CM52 column (30cm x !Scm) that was pre-equilibrated with the same buffer.
    3. Separation of the a and f3 subunits of hemoglobin
  4. May 2019
    1. import java.util.Scanner;

      /**

      • A simple class to run the Magpie class.
      • @author Laurie White
      • @version 6 March 2012 */ public class MagpieRunner2 {

        /**

        • Create a Magpie, give it user input, and print its replies. */ public static void main(String[] args) { Magpie2 maggie = new Magpie2();

          System.out.println (maggie.getGreeting()); Scanner in = new Scanner (System.in); String statement = in.nextLine();

          while (!statement.equals("Bye")) {

           System.out.println (maggie.getResponse(statement));
           statement = in.nextLine();
          

          } }

      }

    1. Riding over the hills, and eating their fill,7 lying a little too long; these things are, perhaps, enough to explain what happened. How­ever, that may be: they woke suddenly from a sleep they had never meant to take. The standing stone was cold, and it cast a long pale shadow. The sun was gleaming through the mist; north, south, and east, the fog was thick, cold and white. The air was silent, heavy and chill.The hobbits8 sprang to their feet in alarm, and ran to the western rim. They found that they were upon an island in the fog. Even as they looked out in dismay towards the setting sun, it sank before their eyes into a white sea, and a cold grey shadow sprang up in the East behind. The fog rolled up to the walls and rose above them, and as it mounted it bent over their heads until it became a roof. They felt as if a trap was closing about them. They packed up as quickly as their chilled fingers would work.Soon they were leading their ponies in single file9 over the rim and down the long northward slope of the hill, down into a foggy sea. As they went down the mist became colder and damper, and their hair hung lank and dripping on their foreheads. When they reached the bottom it was so cold that they halted and got out cloaks and hoods, which soon became bedewed with grey drops. Then, mounting their ponies, they went slowly on again. To prevent their getting separated and wandering in different directions they went in file, with Frodo leading. Suddenly Frodo saw a hopeful sign. On either side ahead a darkness began to loom through the mist; and he guessed that they were at last approaching the gap in the hills. 'Come on! Follow me!' he called back over his shoulder, and he hurried forward. His pony reared, and he fell off. When he looked back he found that he was alone: the others had not fol­lowed him.

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