- May 2019
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Preparation ofE. coli DH5α ultracompetent cells
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Acrylamide (29:1)
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Retroviral based system was used for silencing of PTEN. BOSC23 packaging cells were grown in 100 mm culture dishes upto 80-85% confluency. Retroviral RNA vector containing either scrambled control shRNA or pool of PTEN shRNA along with a PCL-Ampho helper plasmid were co-transfected using Lipofectamine 2000 reagent to generate viral particles. After 48 h, supernatant containing viral particles were used to infect MDA-MB-231 cells in the presence of polybrene (8g/ml). For p53 gene knockdown, TP53 mission shRNAs obtained from Sigma Aldrich (St Louis, MO, USA) were transfected using Lipofectamine 2000 (Invitrogen, USA) and non-targeting shRNAs (Sigma) were used as controls. The cellular homogenates were prepared 36-48 h post transfection and were subjected to immunoblotting to check the levels of protein knockdown
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RNA interference
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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labelled RNAs to N+Hybond membrane (GE Life Science), the radiolabelled rRNA was detected using a phosphorimager (Fuji Film FLA-9000)
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Overnight grown yeast were sub-cultured at 0.2 OD600and growntill 0.8 OD600.Cells equivalent to 1 OD600were harvested and were washed in SC-Ura medium, suspended in 1 mL of SC-Ura medium containing 3μCi/mL of [14C]uracil and pulselabelledfor 15 min at30°C.After incubation the cells were pelleted and a chase was performed by adding SC medium containing 240 mg/L unlabelled uracil. Samples were taken at 0, 1, 5, 10and 20min after the chase, centrifuged at 12000 x gfor 1 min at 4°C, and total RNA was isolated from cells by the hot-phenol techniquedescribed in Section 2.2.8.Equal total RNA was loaded on a 1.2% formaldehyde-agarose gel. After transfer of
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Pulse-chase analysis of rRNA synthesis
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Cells (HEK293T) were transfected with various combinations of plasmids/siRNAsor treated with cisplatin. Cells were washed first with 1X PBSand then with Met-/Cys-1X DMEM supplemented with dialyzed FBS(10%). Cells were then incubated with Met-/Cys-DMEM supplemented with dialyzed FBS in the incubatorfor 1h (met/cysstarvation). Cells were taken out from the incubator,and the culture media was removed. Cells were placed behind the radioactivity protective shield and DMEM supplemented with 35S met/cys (200μCi) was added to the cells. Plates containing radioactive media were then put into the acrylic box and incubated for 1h at 37°C in a CO2 incubator. Plates were taken out and kept behind the radioactivity protective shield;the radioactive media was disposedofin the radioactive liquid waste. One set of cells washarvested for 0 time point, other sets of cells were washed twice with 1X PBS and were incubated with normal medium containing 2mM each of cysteine and methionine. Cell plates were put in the acrylic box and incubated at 37°C in a CO2 incubator. Cells were harvested at different time points. Cells were collected in ice-cold PBS and were lysed using the standard cell lysis protocol. Cell lysate were subjected to immunoprecipitation(IP). IP complex is separated on SDS-gel using standard protocol. The gel was transferred onto PVDF membrane, and the membrane was dried. Dried membrane was exposed in a cassette and the signal was detected using phosphorimager. Later the same blots were probed with specific antibodies
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35S met/cys pulse-chase assay
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Ferric-iron-reduction activity of Xanthomonas oryzaepv. oryzicolawas measured using ferrozine, a chromogenic ferrous iron chelator, as described previously (Velayudhan etal., 2000; Worst et al., 1998). For estimating the ferric reductase activity, Xanthomonas oryzaepv. oryzicolastrains were grown in 20 ml PS medium carrying appropriate antibiotics for 24 h to OD600 of 1. Cell free PS media was incubated under similarcondition to be used as control. Chromogenic ferrous iron chelator, ferrozine was added to a final concentration of 1 mM, and FeCl3was added as ferric iron source to a final concentration of 50 μM, and incubated at 28ºC. At regular time intervals, 1 ml aliquotes were taken from the test culture and control, centrifuged to remove the cells, and absorbance of the magenta coloured Fe2+-ferrozine complex in the cell free culture supernantant was measured at 535 nm by using control supernatant as reference. The Fe2+reduction activity was quantified as micromoles of Fe2+-Ferrozine complex formed
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Assay for ferric reductase activity
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respectively. The resulting constructs pRR14 and pRR15 were transferred in E.coliDH5α. Through triparental mating using pRK600 helper plasmid the construct were transferred in E.coliS17-1. After confirming pRR14 and pRR15 constructs by sequencing, the constructs were then introduced into BXOR1 strain through biparental mating using E. coliS17-1. X. oryzaepv. oryzicolaGUS and GFP reporter strains were selected on PS medium plates containing suitable antibiotics. Since pVO155 cannot replicate in X. oryzaepv. oryzae, ampicillin and kanamycin-resistant colonies were obtained upon chromosomal integration of the plasmid using the cloned DNA sequence as a region of homology. pProbeGTcan replicate independently in Xanthomonasand report for the gene expression
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Glucuronidase (GUS) reporter gene fusion and GFP reporter fusion was created by using the suicide plasmid pVO155 having a promoterless gusAgene (Oke and Long, 1999), and pProbeGThaving a promoterless GFP (Miller et al., 2000). To construct the xsuA::gusAand xsuA::gfptranscriptional fusion, a 611-bp DNA fragment containing the putative promoter of the xssoperon (+213 to −398) was amplified by using the SCRsid_ pProbeGFP_F and SCRsid_ pProbeGFP_R primers (Table 2.2). This promoter fragment was subsequently digested with HindIII and BamHI,and directionally cloned upstream of the promoterless gusAand gfpgene in pVO155 and pProbeGTplasmids to create the xsuA::gusAand xsuA::pProbeGT(gfp) reporter constructs pRR1
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Construction of xsuA::gusAand xsuA::gfp strains in X. oryzaepv. oryzicola background
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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To perform immunoblotting or western blotting, appropriate amounts of total protein(ranging from 20-40 μg) were separated ona SDS-PAGE gel of 12%acrylamide concentration in Tris-Glycine-SDS gel running buffer. Protein separation was done at 70-100 V for 2-3 h using a MINI PROTEAN®3 electrophoresis unit (Bio-Rad). Followingseparation, proteins weretransferredto polyvinylidene difluoride (PVDF) membrane, using a Bio-Rad Mini Trans-Blot electrophoretic transfer unit in Tris-Glycine transfer buffer at 4⁰C. Before setting transfer assembly, PVDF membrane was first activated in 100% methanolfollowedby washesin the transfer buffer. The transfer assembly was set inaBio-Rad Mini gel holder cassette (170-3931)according to manufacturer’s instructions. The transfer time and current settings varied depending on the size oftheprotein of interest. Post transfer, membranes wereseparated from the assembly and kept for blocking intheblocking buffer (0.1 % Tween-20, 5% w/v fat-free skimmed milk in 1X TBS) for 1 h at room temperature with shaking. Next,membranes wereincubated with appropriate dilutions of primary antibodiesin the blocking buffereitherfor 3-4 h at room temperature or overnight at 4°C with gentle shaking. Post incubation,membranes were washed thrice with 1X TBS-T, 10 min each,with constant agitation. After washes, membraneswere incubated with appropriate dilutions of secondary antibodiesconjugated with horseradish peroxidase (HRP) for 1 h at room temperature with gentle shaking. Next, membranes were washed thrice with 1X TBS-T, 10 min each,with constant agitation. To visualize proteins, membranes were removed from TBS-T, and theHRP substrate ECL plus (Amersham Biosciences, RPN2232) was uniformly added on top of the membrane. Chemiluminescent signalswere captured in the western blot imaging system (FluorChemTME system)
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For protein extraction, cells were spun down at 4,000 rpm for 5 min and washed with ice-cold water. The cell pellet was resuspended in 250-500 μl of homogenisation buffer which contained50 mM Tris (pH 7.5), 2 mM EDTA, 1 mM phenylmethylsulphonyl fluoride (PMSF) (serine protease inhibitor), 10 mM sodium fluoride (serine/threonine and acid phosphatases inhibitor), 1 mM sodium orthovanadate (Tyrosine and alakaline phosphatases inhibitor) and 1X protease inhibitor cocktail (Sigma, P 8215). The cell suspensison was transferred to a 1.5 ml centrifuge tube and equal amounts of glass beads (0.5 mm size) were added. Cells were lysed mechanically by bead-beatinghomogenizer (MP Biomedicals, FastPrep®-24) atthemaximum speed for 60 seconds, five times each,with intermittent cooling on ice.After lysis, tubes were punctured at the bottom with the helpofasurgical needle, and the lysed cell suspension was collected in a fresh microcentrifuge tubes by putting the punctured tubes on top of the fresh tubes and centrifuging them at 3,000 rpm for 10 min. The supernatant was transferred toafresh microcentrifugetubeandprotein concentrationwasestimated usingtheBCA protein assay kit (Thermo scientific). Protein preparations werestored at -20°C until use
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Total protein extraction and immunoblotting
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Total protein extraction and immunoblotting
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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the selection marker.Knockdown was confirmed by immunoblot analysis with an IP6K1 specific antibody (Table 2.3) as described in Section 2.2.10
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lines were used for stable knockdown of IP6K1 expression. Viral particles harboring either non-targeting control or IP6K1directed shRNA were used to infect HeLa or HCT116 cell lines at 0.5 MOI, following treatment with polybrene (8 μg/mL)for 2 h.After 48 h, transduced cells were selected with 2 μg/mL puromycin. Medium was changed twice a week and observed for colony formation. After reaching the optimum growth, selected cells were maintained in DMEM supplemented with 10% FBS and 1 μg/mL puromycin as
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Generation of stable cell lines expressing shIP6K1-HeLa and HCT116 cell
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HEK293T packaging cellswere seeded at 30-40% confluency in 60 mm dishes. After 24 h, cells were co-transfected with three plasmids required for viral production i.e. VSV-G, psPAX2 (Addgene plasmid # 12260) and pLKO.1-puro-non-targeting and shIP6K1 clones using polyethyleniminereagent(PEI) and incubated at 37°C and 5% CO2 for virion formation. After 48 h, viral particles were harvested by collecting supernatant and filtered througha 0.45 μm syringe filter unit. Viral stock was aliquoted and stored at -80°C for further use. Viral titer was approximated on the number of cells plated for the production of lentivirus. Calculations were done as per Cell Bio Labs instruction. 2 x 106cells will yield 107infectious units/mL. All necessary precautions were taken while generating lentiviral particles such as wearing mask, double gloves, and sterile filter tips. All the consumables used were bleached (1% sodium hypochlorite solution) at least 1 h before being discarded
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Generation of lentiviral particle containing shRNAagainst human IP6K1-
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Generation of stable cells expressing shIP6K1 in HeLa and HCT116
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- Apr 2019
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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RRID:ZFIN_ZDB-GENO-150504-12
Curator: @evieth
Resource used:
RRID:ZFIN_ZDB-GENO-150504-12
SciCrunch record: RRID:ZFIN_ZDB-GENO-150504-12
Alternate resolvers: SciCrunch xml N2T identifiers.org
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elifesciences.org elifesciences.org
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RRID:ZFIN_ZDB-GENO-060811-12
Curator: @kaitlinsung
Resource used:
RRID:ZFIN_ZDB-GENO-060811-12
SciCrunch record: RRID:ZFIN_ZDB-GENO-060811-12
Alternate resolvers: SciCrunch xml N2T identifiers.org
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- Mar 2019
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www.edweek.org www.edweek.org
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Personal Learning Environments (PLE) applied to K-12 classroom
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www.getmagicbox.com www.getmagicbox.com
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personalized mobile learning solutions to create effective learning paths This appears to blend personalized learning and mobile learning. It is prepared by a specific vendor, MagicBox, so they might be assumed to have their own agenda. This page describes some of the affordances of personalized mobile learning, such as the capacity to track and presumably respond to learner preferences. rating 2/5
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- Feb 2019
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static1.squarespace.com static1.squarespace.com
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he is at the same time, capable of becom+ ing a greater enemy,
"For those who have been given more, more will be expected." Luke 12:48
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mozilla.github.io mozilla.github.io
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They can evaluate web content, and identify what is useful and trustworthy
This should be taught throughout k-12 schooling. Learnng this in college was super helpful but it was taught a little late for me. I know now how to choose sources that present good information but growing up I wouldnt have been able to do that
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- Nov 2018
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edtechmagazine.com edtechmagazine.com
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What K–12 Administrators Should Think About When Integrating Classroom Tech
This article describes how school districts who wish to integrate more technology into their classrooms should approach the integration. Rating: 4/5
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- Oct 2018
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carolblack.org carolblack.org
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Evaluation, when it is not asked for, and when it has consequences as it does in school, is a threat. It narrows the mind... it inhibits new learning, new insights, and creative thought—the very processes that some people think school is supposed to promote. -- Peter Gray
. . .
Why is it clear to us that it's degrading and objectifying to measure and rank a girl’s physical body on a numeric scale, but we think it’s perfectly okay to measure and rank her mind that way?
. . .
what an oak tree actually needs is not your opinion but soil and water and light and air, and what a child needs is love and stories and tools and conversation and support and guidance and access to nature and culture and the world. If a kid asks for your feedback, by all means you can give it; it would be impolite not to. But what we should be measuring and comparing is not our children but the quality of the learning environments we provide for them.
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- Sep 2018
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primarydocuments.ca primarydocuments.ca
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bishop gave judgment, declaring the marriage null in a canonical sense. Regarded in a civil point of view, the marriage was still valid until it should have been declared null by a civil tribunal. It became necessary, therefore, to carry the cause before the Superior Court, and my honorable friend, the member for Because, who took the case in hand with his usual zeal and legal address, obtained from the court, after a suitable inquiry, a judgment declaring the marred null in a civil sense, and ordering that it should be registered as such in all places where it should be needful. If this affair had occurred in Upper Canada, what recourse would the parties have had ? The parties being Catholics, the case would have been brought before the bishop, who would also have declared the marriage null after suitable inquiry; but the cause would not have had the same conclusion in the civil court, particularly had it depended on certain impediments which have force in Lower Canada, but none in Upper Canada. It would have become necessary to go to Parliament to pray for an act, which, in a Catholic point of view, would be a mere decree of separation, but which the Parliament would have termed an act of divorce. This power to grant a separation is therefore necessarily vested in the Parliament, by whatever names such separation may be designated, and we are not to be reproached for the interpretation which others may give to such name, different from that which we assign to it. I thought it right to make myself understood on this point, because I do not choose that people should be able to say we are afraid of explaining our position with regard to the question of divorce and marriage, and I believe that I have shown that our position is consistent with our religious laws and our principles as Catholics. I regret that I have dwelt so long on the matters touched upon by the honorable member for Hochelaga ; but after his speech, and considering the position he assumed, he must have expected an answer.
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HON. SOL. GEN. LANGEVIN—I beg your pardon, it means that a marriage contracted in no matter what part of the Confederacy, will be valid in Lower Canada, if contracted according to the laws of the country in which it takes place ; but also, when a marriage is contracted in any province contrary to its laws, although in conformity with the laws of another province, it will not be considered valid. Let us now examine the question of divorce. We do not intend either to establish or to recognize a new right ; we do not mean to admit a thing to which we have constantly refused to assent, but at the Conference the question arose, which legislature should exercise the different powers which already exist in the constitutions of the different provinces. Now, among these powers which have been already and frequently exercised de facto, is this of divorce. As a member of the Conference, without admitting or creating any new right in this behalf, and while declaring, as I now do, that as Catholics we acknowledge BO power of divorce, I found that we were to decide in what legislative body the authority should be lodged which we found in our Constitutions. After mature consideration, we resolved to leave it in the Central Legislature, thinking thereby to increase the difficulties of a procedure which is at present so easy. We thought then, as we still think, that in this we took the most prudent course. The following illustration will prove this still more forcibly. I t is known to the House how zealous a partisan the honorable member for Brome (Mr. DUNKIN) is of the cause of temperance. Well, we will suppose that the honorable gentleman were present as a member of a municipal council in which it was to be decided whether all the taverns in a very populous part of the parish, which could not be suppressed, should be banished to a remote corner of the parish, where they would no longer be a temptation and a stumbling-block ; would he not vote for such a measure ? Would he not send them to a place where they would be least accessible to the population, and would he not think he had done a meritorious act, an act worthy of a good friend of the temperance cause ? Just so in a question of divorce ; the case is exactly analogous. We found this power existing in the constitutions of the different provinces, and not being able to get rid of it, we wished to banish it as far from us as possible. One thing it would be vain to deny, namely, that although we, as Catholics, do not admit the liberty of divorce, although we hold the marriage bond to be indissoluble, yet there are cases in which we both admit and require the annulling of the marriage tie—in cases, for instance, where a marriage has been contracted within the prohibited degrees without the necessary dispensations. An instance of this occurred very recently. A few months since, an individual belonging to my county, who had married a young girl of a neighboring parish, without being aware at the time of his marriage of the relationship which existed between him and his wife, found out several months afterwards that they were related in such a degree that they required a dispensation from the bishop. That dispensation had not been obtained. He spoke of it to his wife, who refused to apply for a dispensation, as a step towards the legal celebration of their marriage. It became necessary, therefore, to have the marriage annulled. The affair was brought before the Ecclesiastical Court, and, after a minute investigation, the diocesan
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HON. SOL. GEN. LANGEVIN—The honorable member for Verchères does not choose to be convinced ; so I will make no further attempt to convince him. The resolution in question signifies just what I have stated.
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may be summed up as follows :—The Central Parliament may decide that any marriage contracted in Upper Canada, or in any other of the Confederated Provinces, in accordance with the laws of the country in which it was contracted, although that law might be different from ours, should be deemed valid in Lower Canada in case the parties should come to reside there, and vice versa.
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The honorable gentleman has asked the Government what meaning was to be attached to the word ” marriage,” where it occurred in the Constitution. He desired to know whether the Government proposed to leave to the Central Government the right of deciding at what age, for example, marriage might be contracted. I will now answer the honorable gentleman as categorically as possible, for I am anxious to be understood, not only in this House, but also by all those who may hereafter read the report of our proceedings. And first of all I will prove that civil rights form part of those which, by article 43 (paragraph 15) of the resolutions, are guaranteed to Lower Canada. This paragraph reads as follows :— 15. Property and civil rights, excepting those portions thereof assigned to the General Parliament. Well, amongst these rights are all the civil laws of Lower Canada, and among these latter those which relate to marriage; now it was of the highest importance that it should be so under the proposed system, and therefore the members from Lower Canada at the Conference took great care to obtain the reservation to the Local Government of this important right, and in consent-‘ ing to allow the word ” marriage ” after the word “divorce,” the delegates have not proposed to take away with one hand from the Local Legislature what they had reserved to it by the other. So that the word ” marriage,” placed where it is among the powers of the Central Parliament, has not the extended signification which was sought to be given to it by the honorable member. With the view of being more explicit, I now propose to read how the word marriage is proposed to be understood :— The word marriage has been placed in the draft of the proposed Constitution to invest the Federal Parliament with the right of declaring what marriages shall be held and deemed to be valid throughout the whole extent of the Confederacy, without, however, interfering in any particular with the doctrines or rites of the religious creeds to which the contracting parties may belong. This is a point of great importance, and the French Canadian members ought to rejoice to see that their fellow-countrymen in the Government have not failed in their duty on a question of so serious a nature. On many other points many of them will doubtless claim that we have not thoroughly fufilled our duty, but as regards the matter in question there can be no difference of opinion, as I we have all a common rule to guide us ; and I repeat that they ought to rejoice that their co-religionists in the Conference have not been found wanting on this occasion. The whole
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primarydocuments.ca primarydocuments.ca
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Remarks have also been made about the laws of divorce and marriage, and the honorable member for the division of DeLanaudière (Hon. Mr. OLIVIER) told us that the Conference had done well in transferring the power of divorce to the General Government. On his part, I think this was a wise view of the question, and I am glad to have the opportunity of now telling him so. He was, however, very uneasy about the word ” marriage.” Well, I will try to put him right and at his ease on that point; and I will give him the answer as I find it put down in writing, so that no possible misunderstanding may continue to exist. If the honorable gentleman will but take his pen, he will be able to note my answer :—” The word ‘ marriage’ has been inserted to give the General Legislature the right to decide what form of marriage will be legal in all parts of the Confederation, without in any way interfering with the rules and prescriptions of the Church to which the contracting parties belong.”
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One of them is a matter in which Lower Canada is somewhat peculiarly interested— the system of marriage and divorce, which, I see, is to be left in the hands of the Federal Government.
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primarydocuments.ca primarydocuments.ca
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what is meant by the regulation of the law of divorce ; but what is meant by the regulation of the marriage question ? Is the General Government to be at liberty to set aside all that we have been in the habit of doing in Lower Canada in this respect ? Will the General Government have the power to determine the degree of relationship and the age beyond which parties may marry, as well as the consent which will be required to make a marriage valid ?
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primarydocuments.ca primarydocuments.ca
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What shall I say on the subject of marriage—the basis of all our institutions ? Is it not dangerous to have it at the mercy of the Federal Government ? We shall soon be told probably that it is but a sounding affair, and before long, mayors will take the place of the curés, and will celebrate the marriages of their constituents. Our laws which regulate our marriages at present are very important to us, and are based on the Roman law. These are the only laws suitable to Canadians, and the wise provisions characterizing them were the fruit of the experience of several ages. We should not incur the risk of any change in them by a legislature, the majority of whose members do not hold our opinions on this subject.
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- Aug 2018
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primarydocuments.ca primarydocuments.ca
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Take, for instance, the question of the fisheries. Article 17, of the 29th resolution, gives to the Federal Parliament the power of legislating on the ” sea coast and inland fisheries.” Under the 8th article of the 43rd resolution, the local legislatures will also have the right of legislating on the ” sea coast and inland fisheries.” Thus the local legislatures and the Federal Legislature will have the right to legislate on the same subjects. And if the laws they make are in opposition the one to the other, what will be the result ?
§.91(12) of the Constitution Act, 1867.
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The 29th section of the scheme submitted to us says : ” The Federal Parliament shall have the power of making laws for the peace, the well-being, and the good government of the Confederate provinces, and in particular in respect of the following matters.” The powers of the Federal Government will be in reality unlimited. The fact of the enumeration of these thirty-seven heads does not in the least restrain the power of the Federal Government from legislating on everything. The exceptions are few. I would ask the Honorable Premier, for instance, whether the Federal Government has not the power to enact that marriage is a civil contract ? He cannot deny it, and I do not believe that that clause will in any way suit Lower Canada. In a matter of divorce, I consider that the power of legislating upon it ought to be vested in the Federal Government ; but as to the passing of a marriage act, we have the authority of the past to convince us that Lower Canada will never be satisfied with what is proposed in the plan of Confederation. On a former occasion, when a member of the Parliament of Canada moved to enact that marriage should be made a civil contract, all the members for Lower Canada voted against the motion, and the whole country was opposed to it. I shall also inquire whether the Federal Government will not have the right to enact that religious corporations shall no longer exist in the country, or that they shall not be allowed to hold real property, except what is absolutely necessary for their lodging accommodation. According to the resolutions which have been submitted to us, the Federal Government would certainly have this right. It has been said that article 15 of the 43rd resolution replies to this objection, but I can see nothing in that article which restricts the right of the Federal Government to legislate on this matter. The 43rd resolution defines the powers of the local governments, and article 15 of that resolution declares that they may make laws respecting ” property and civil rights, excepting those portions thereof assigned to the General Parliament.” That article reserves to the local legislatures nothing relative to religious corporations, and the Federal Government would have full power to decree that those corporations shall not hold immovable property. The supreme power is that which has the right to legislate upon, and regulate the existence of, the corporations in question, and they can only possess civil rights so long as the Government permits them to exist. The same might be said of most of the institutions to which Lower Canada is attached. I am therefore right in saying that, so far as those things which Lower Canada most holds to are concerned, Confederation is in fact a Legislative union, because upon the Federal Government is conferred the right of legislating upon those subjects which Lower Canada holds most dear.
Preamble and §§.91(26)(29), 92(11)(12)(13), and 93 of the Constitution Act, 1867.
Tags
- Section 91(12) of the Constitution Act 1867
- Preamble of the Constitution Act 1867
- Section 92(12) of the Constitution Act 1867
- Section 91(26) of the Constitution Act 1867
- Section 92(11) of the Constitution Act 1867
- Section 92(13) of the Constitution Act 1867
- Section 91(29) of the Constitution Act 1867
Annotators
URL
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theundefeated.com theundefeated.com
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However, as admirable as James’ philanthropic efforts are, they are not a solution to the problems in public education.
I don't think James ever claimed he was solving "the problems in public education." How about he's shining a light on ways increased public funding could stimulate public education?
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- Jul 2018
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Local file Local file
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9
Step 11:
Now for the side panels of the desk. Secure the railing using 2 110853 into the smaller pre-drilled holes. Consult the graph for clarification.
Step 12:
Secure the 2 124639 into the back panel of the desk using a Phillip-Head screwdriver. Consult the graph for proper alignment.
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- Apr 2018
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primarydocuments.ca primarydocuments.ca
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there might be some misunderstanding and difference of opinion, as for example those clauses by one of which it was stated that the civil laws of the country were to be under the control of the local governments, and by the other of which the law of marriage was placed under the control of the General Government. The law of marriage pervaded the whole civil code, and he wanted to know how it could be placed under a different legislature from that which was to regulate the rest of the civil law.
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- Mar 2018
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primarydocuments.ca primarydocuments.ca
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It was adopted by the Imperial Legislature with the view of remedying difficulties which then existed between the two Provinces. The inherent defect in the Imperial Act for the Union of the two Canadas was this : it attempted to combine the federal principle with unity of action. It endeavoured to give equal representation to the two sections of the Province, while it brought them together for the purpose of dealing as one with all subjects both (general and local,
§§. 3 and 12 of the Union Act, 1840.
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Those claims were in themselves undoubtedly founded in justice— but at the same time there was great reason in the objections taken to them—they involved an interference with the Federal principle recognized in the Union Act, an interference which amounted to an entire change in the principles on which the Government of the country was to be administered, and could not be received otherwise than with dread by a large class, if not by the whole of the population of Lower Canada.
§§. 3 and 12 of the Union Act, 1840.
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should have been a concession to Upper Canada, of additional members in proportion to its population, but that concession would, as be had already remarked, have been an invasion of the Federal principle, contained in the Union Act, and would unquestionably have been represented to the uttermost by a large proportion if not by the whole of Lower Canada.
§§. 3 and 12 of the Union Act, 1840; §§. 51 and 52 of the Constitution Act, 1867.
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www.edsurge.com www.edsurge.com
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Pearson out of US k-12 market
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- Nov 2017
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www.edtechteam.com www.edtechteam.com
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7 Shifts to Closing the Digital Divide
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- Aug 2017
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blogs.edweek.org blogs.edweek.org
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Can an 'Open' Math Curriculum Compete With Commercial Publishers?
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- Jul 2017
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www.huffingtonpost.com www.huffingtonpost.com
- Jun 2017
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motherboard.vice.com motherboard.vice.com
- May 2017
- Mar 2017
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www.washingtonpost.com www.washingtonpost.com
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www.theatlantic.com www.theatlantic.com
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robertscribbler.com robertscribbler.com
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www.businessinsider.com www.businessinsider.com
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www.thevintagenews.com www.thevintagenews.com
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www.theguardian.com www.theguardian.com
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www.bloomberg.com www.bloomberg.com
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Wed Mar 29 2017 20:17:55 GMT-0400 (EDT)
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Wed Mar 29 2017 10:42:16 GMT-0400 (EDT)
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www.washingtonpost.com www.washingtonpost.com
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Wed Mar 29 2017 10:39:16 GMT-0400 (EDT)
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Wed Mar 29 2017 10:37:28 GMT-0400 (EDT)
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Wed Mar 29 2017 10:36:09 GMT-0400 (EDT)
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www.cbsnews.com www.cbsnews.com
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Tue Mar 28 2017 13:12:59 GMT-0400 (Eastern Daylight Time)
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Tue Mar 28 2017 08:53:08 GMT-0400 (Eastern Daylight Time)
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Tue Mar 28 2017 13:12:31 GMT-0400 (Eastern Daylight Time)
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www.foxnews.com www.foxnews.com
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Tue Mar 28 2017 13:11:47 GMT-0400 (Eastern Daylight Time)
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Tue Mar 28 2017 09:09:15 GMT-0400 (Eastern Daylight Time)
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www.google.com www.google.com
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Tue Mar 28 2017 09:01:17 GMT-0400 (Eastern Daylight Time)
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Tue Mar 28 2017 09:00:57 GMT-0400 (Eastern Daylight Time)
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www.reuters.com www.reuters.com
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Tue Mar 28 2017 08:55:52 GMT-0400 (Eastern Daylight Time)
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www.google.com www.google.com
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Mon Mar 27 2017 10:22:09 GMT-0400 (Eastern Daylight Time)
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www.youtube.com www.youtube.com
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Mon Mar 27 2017 08:19:42 GMT-0400 (Eastern Daylight Time)
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www.cnbc.com www.cnbc.com
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Thu Mar 23 2017 04:14:30 GMT-0400 (Eastern Daylight Time)
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sofrep.com sofrep.com
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Thu Mar 23 2017 04:13:25 GMT-0400 (Eastern Daylight Time)
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Thu Mar 23 2017 04:12:54 GMT-0400 (Eastern Daylight Time)
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www.newyorker.com www.newyorker.com
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www.hotnews.ro www.hotnews.ro
- Jan 2017
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www.huffingtonpost.com www.huffingtonpost.com
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Sun Jan 08 2017 20:25:47 GMT-0800 (Pacific Standard Time)
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www.tandfonline.com www.tandfonline.com
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Sun Jan 08 2017 18:29:13 GMT-0800 (Pacific Standard Time)
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www.openculture.com www.openculture.com
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Parents and students in the Mountain View school district consider their use of math software called Teach to One to be a failure.
The Mountain View school district apparently budgeted $521,000 to implement and operate this new-fangled math program in two local schools. Had they adequately beta tested the program beforehand, the school district might have discovered that Teach to One teaches math (we’ve found) in a disjointed, erratic, and non-linear fashion that leaves many students baffled and disenchanted with math. The program contains errors in the math it teaches. Parents end up having to teach kids math at home and make up for the program’s deficiencies. And all the while, the math teachers get relegated to “managing the Teach to One program rather than to providing direct instruction” themselves.
Sounds like they would have been much better off to just use http://khanacademy.org or http://schoolyourself.org So why are they using this expensive, seemingly untested service?
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www.huffingtonpost.com www.huffingtonpost.com
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Sara Holbrook had two of her poems used on the Texas state assessment tests. She verifies what I thought as a student. The questions are ridiculous. The test makers seem to think that their interpretation of a work is the only interpretation, and that they can read the author's mind and know their intent.
"Texas paid Pearson $500 million bucks to administer the tests". Is that right? Was that for just one year? What else could we do with $500 million?
She mentions a study showing that the results of another standardized test could be predicted pretty well using just three data points about families in the community: the percentage with income over $200K; the percentage in poverty; the percentage with bachelor's degrees. So the standardized test tells you nothing that you can't guess by looking at local incomes and education levels.
What a scam.
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www.infowars.com www.infowars.com
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Sun Jan 01 2017 15:37:54 GMT-0800 (Pacific Standard Time)
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www.snopes.com www.snopes.com
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Sun Jan 01 2017 13:59:12 GMT-0800 (Pacific Standard Time)
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- Dec 2016
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mocanu13.blogspot.com mocanu13.blogspot.com
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Fri Dec 30 2016 10:31:19 GMT-0800 (Pacific Standard Time)
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hacklearning.org hacklearning.org
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Since there is no proven “right way” to assess and track student learning, the most effective option is to turn this responsibility over to the students. Because learning should stem from intrinsic motivation, teachers can spend time showing students how to track the feedback and their progress from long-term learning experiences. This way students will learn to reflect on their growth, set better goals, and be accountable for their own growth.
-- Starr Sackstein<br> http://hackingassessment.com/<br> http://hackinghomework.com/
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bipartisanreport.com bipartisanreport.com
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Tue Dec 27 2016 16:13:04 GMT-0800 (Pacific Standard Time)
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www.politico.com www.politico.com
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Tue Dec 27 2016 10:36:57 GMT-0800 (Pacific Standard Time)
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www.independent.co.uk www.independent.co.uk
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Sun Dec 25 2016 14:39:56 GMT-0800 (Pacific Standard Time)
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www.washingtonpost.com www.washingtonpost.com
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Sun Dec 25 2016 13:34:32 GMT-0800 (Pacific Standard Time)
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www.zerohedge.com www.zerohedge.com
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Sun Dec 25 2016 13:26:25 GMT-0800 (Pacific Standard Time)
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www.infowars.com www.infowars.com
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Sun Dec 25 2016 13:23:56 GMT-0800 (Pacific Standard Time)
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www.philly.com www.philly.com
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Sat Dec 24 2016 13:32:06 GMT-0800 (Pacific Standard Time)
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www.ncleg.net www.ncleg.net
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www.ncleg.net www.ncleg.net
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www.dailymail.co.uk www.dailymail.co.uk
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sites.google.com sites.google.com
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She said I am the one who will dance on the floor in the round
The significance of the "Billie Jean" when it came out was do to the fact that the song was about a person being told that they are some kids father.
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- Oct 2016
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bullshit.ist bullshit.ist
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Sunil Singh asks us to stop promoting mathematics based on its current applications in business and science. Math is an art that should be enjoyed for its own sake.
This reminded me of A Mathematician's Lament by Paul Lockhart. This is a 25-page essay which was later worked into a 140-page book. (And Sunil Singh has read at least one of them. He credits Lockhart in one of the replies.)
It also reminds me of this article on the history of Gaussian elimination and the birth of matrix algebra. Newton's algebra text included instructions for solving systems of equations -- but it didn't have much practical use until later. (Silly word problems are as old as mathematics.)
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We should let people learn at their own pace. We should neither rush them, nor hold them back. If they show a talent, then encouraging them to push themselves is fine.
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medium.com medium.com
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Math isn't for everyone, and that's fine. The same is true of any other subject. We should help people learn what they are interested in learning.
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- Sep 2016
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Local file Local file
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Okay,incaseyoudidn’tcatchthat,listentothatlastpartonemoretime.JaystartedmakingupsomeconversationthathadwithAdnanabouthisgloves,aconversationJayneverevermentionsagainandhasnopoint
Susan is obvious on how she is bias towards Adnan being not guilty. She constantly is pointing out that Jay lies in his story. She is good at pretending like she is not bias by giving statements with Adnan recorded voice being involved in the situation, but it all leads back her pointing the finger of guilt to Jay. With this being told by Susan it is really convincing that Jay's story has many more holes and things that do not exactly match up. When she brings up the Jay interview involving taping and Jays's pauses in the interview, she really sold me on the idea that Adnan was wrongfully tried. Susan really did successfully used pathos, ethos, logos. Using the logic of the common person, it is very clear that something was wrong in that interview.
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Buttherealquestionforalotofpeoplehasalwaysbeen:wereJayandAdnanfriends?
This seems to be the author's claim of the purpose of the episode to describe what she is investigating. She seems to want to keep her ethos by not saying anything particularly bias to her opinion to far. This podcast is very similar to Koenig's and has many similarities with how she uses the evidence and how she presents it.
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cdn.nmc.org cdn.nmc.org
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Ashift is taking place in schools all over the world as learners are exploring subject matter through the act of creation rather than the consumption of conten
So interesting to see this "realization" included in the K-12 report but not in the HE report. Fostering curiousity, interest, creativity, and ownership. Short jump to an open pedagogy model but pretty unclear that's where this is coming from.
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- Jul 2016
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neil.fraser.name neil.fraser.name
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Neil Fraser says Vietnam is doing well with computer science education.
"If grade 5 students in Vietnam are performing at least on par with their grade 11 peers in the USA, what does grade 11 in Vietnam look like? I walked into a high school CS class, again without any advance notice. The class was working on the assignment below (partially translated by their teacher for my benefit afterwards). Given a data file describing a maze with diagonal walls, count the number of enclosed areas, and measure the size of the largest one."
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www.brilliant-insane.com www.brilliant-insane.com
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"If given the opportunity all teachers would stop grading their students. You’ll never find a teacher who loves grading papers, projects or tests. In 20 years as a classroom teacher, I heard more complaints about grading than anything else."
"So, if they hate it so much, why don’t teachers stop grading? Because parents, administrators, and bureaucrats won’t let them."
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www.brilliant-insane.com www.brilliant-insane.com
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"Real gifted education (not gifted programs) involves seeing every student as an individual, finding out what they need, what they want to learn, and what they care about, and then adapting the instructional environment and curriculum to those needs, wants, and passions."
"There’s no reason we can’t do this for everyone, letting gifted students soar without the downsides of selective gifted programs."
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- May 2016
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“Our whole reason for doing this is a social justice reason,” he said. The organization wants to make high-quality material available for free to all students.
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- Apr 2016
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medium.com medium.com
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Providing Transformative Personalized Student Learning Opportunities as a Path Toward Equity
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willrichardson.com willrichardson.com
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9 Elephants in the (Class)Room That Should “Unsettle” Us
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- Mar 2016
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www.edsurge.com www.edsurge.com
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“We see kids in their cars in the parking lot at night and on weekends,” says Buddy Berry, superintendent of Eminence Independent Schools. They’re there, he says, because they can access the Internet using the school’s wireless network—something many don’t have at home.
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- Jan 2016
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www.whitehouse.gov www.whitehouse.gov
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educators and business leaders are increasingly recognizing that CS is a “new basic” skill necessary for economic opportunity. The President referenced his Computer Science for All Initiative, which provides $4 billion in funding for states and $100 million directly for districts in his upcoming budget; and invests more than $135 million beginning this year by the National Science Foundation and the Corporation for National and Community Service to support and train CS teachers.
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www.edweek.org www.edweek.org
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Gooru, a Redwood City, Calif.-based nonprofit that relies on educators as "crowdsourcers." The Gooru platform is geared toward teachers building collections of open-ed multimedia resources and students directing their own learning.
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Flood of Open Education Resources Challenges Educators
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- Nov 2015
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www.bpsedtech.org www.bpsedtech.org
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Better Lesson: A Common Core Aligned OER
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m.cjonline.com m.cjonline.com
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USD 501 teachers looking to replace science textbooks with Web-based instruction
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tech.ed.gov tech.ed.gov
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“Instead of having one prescribed way to do things that comes from a textbook, kids can do things where they’re truly interested,” says Lori Secrist. “When they’re truly interested, they’re engaged. And when they’re engaged, they learn.”
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- Sep 2015
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www.gutenberg.org www.gutenberg.org
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Of Man's first disobedience, and the fruit Of that forbidden tree whose mortal taste Brought death into the World, and all our woe, With loss of Eden, till one greater Man Restore us, and regain the blissful seat, Sing, Heavenly Muse, that, on the secret top Of Oreb, or of Sinai, didst inspire That shepherd who first taught the chosen seed In the beginning how the heavens and earth Rose out of Chaos: or, if Sion hill Delight thee more, and Siloa's brook that flowed Fast by the oracle of God, I thence Invoke thy aid to my adventurous song, That with no middle flight intends to soar Above th' Aonian mount, while it pursues Things unattempted yet in prose or rhyme.
There are sixteen lines of verse before Milton reaches his first period. He uses this first sentence to call forth the "Heavenly Muse" (possibly the Holy Spirit) to help him compose his "adventurous song."
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- Jun 2015
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www.edweb.net www.edweb.net
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webinar for k-12 oer
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thejournal.com thejournal.com
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Open source curriculum
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