Der kurz vor der COP16 zur Biodiversität veröffentlichte Living Planet Index zeigt das Ausmaß des Biodiversitätsverlusts in den vergangenen 50 Jahren, auch wenn an den dabei angewendeten statistischen Verfahren starke Zweifel bestehen. Die Wirbeltier-Populationen haben nach diesem Index um 73% abgenommen, am stärksten in Lateinamerika und der Karibik. Die wichtigste Ursache ist die veränderte Landnutzung. https://www.theguardian.com/environment/2024/oct/10/collapsing-wildlife-populations-points-no-return-living-planet-report-wwf-zsl-warns

NEB says

Digestion is recommended whenever DNA input is greater than 75 ng Source

NEB says that biorad recommends these enzymes: AluI, CviQI, HaeIII, HindIII-HF, MseI

More guidelines

• Assemble ddPCR reactions at room temperature, this allows the restriction enzyme to digest the gDNA during the reaction set-up period
• Prepare reaction mixes as you would for a standard ddPCR reaction. Add 0.5–1 μL of each restriction enzyme (5–20 units, depending on enzyme concentration) to the reaction mixture
• After set-up, simply continue droplet generation as normal
• Restriction enzyme will be inactivated during first PCR denaturation step

Refer to this protocol mentioned in the BioRad page, annotation

• for: definition epistemic fragmentation, no agreement on basic facts, political polarization, adjacency - epistemic fragmentation - polarization - democracy

• definition: epistemic fragmentation

  • when major parts of society disagree on basic facts
• adjacency between
  • epistemic fragmentation
  • polarization
  • democracy
• adjacency statement
  • democracy is critical to solving our challenges but it won't function if there is so much epistemic fragmentation that we can't agree on basic facts

• for: quote, quote - Ethan Zuckerman, quote - fragmentation and polarization, Indyweb - support, MIT Center for Civic Media, Global Voices
• quote
  • We need mass innovation in design of social tools that help us
    • bridge fragmentation and polarization,
    • bring diversity into our media landscapes and
    • help find common ground between disparate groups.
  • With these as conscious design goals,
    • technology could be a powerful positive force for civic change.
  • If we don’t take this challenge seriously and assume that we’re stuck with mass-market tools,
    • we won’t see positive civic outcomes from technological tools.”
• author
  • Ethan Zuckerman
    • director of MIT’s Center for Civic Media and
    • co-founder of Global Voices

!- future global fragmentation : by culture - Michaux believes people will fragment in the future along cultural boundaries as we move through tumultuous transition. This makes sense as ingroups will naturally form - this should be further explored to explore implications: - will we get political polarization? At what level? National, regional, city / community scale? - what implications will this have on cooperation and sharing? will it create policy gridlock? Will it become even more urgent to educate everyone on a Deep Humanity type of open praxis that finds common human denominators (CHD)?

Interesting to note the time period of this peak, which broadly coincides with Brown vs. Board of Education and desegregation in the 1970s. Did the fluorescence of these others begin as a means of better segregating students either racially or economically?

Push back, sure, but where? Where would we find this push back? The comments section only has a few tidbits. Perhaps the rest is on Twitter, Facebook, or some other social silo where the conversation is fraught-fully fragmented. Your own social capital is thus spread out and not easily compiled or compounded. As a result I wonder who may or may not have read this piece...

Garmendia, A., & Alfonso, S. L. (2020). Popular Reactions To External Threats in Federations. https://doi.org/10.31235/osf.io/qyjtm

Pham, T. M., Kondor, I., Hanel, R., & Thurner, S. (2020). The effect of social balance on social fragmentation. ArXiv:2005.01815 [Nlin, Physics:Physics]. http://arxiv.org/abs/2005.01815