8,556 Matching Annotations
- Jul 2019
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Growth
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Experimental Set up
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- Jun 2019
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www.railstutorial.org www.railstutorial.org
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At each stage of developing the sample application, we will write small, bite-sized pieces of code—simple enough to understand, yet novel enough to be challenging. The cumulative effect will be a deeper, more flexible knowledge of Rails, giving you a good background for writing nearly any type of web application.
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Following the scaffolding approach risks turning you into a virtuoso script generator with little (and brittle) actual knowledge of Rails.
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URL
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krishikosh.egranth.ac.in krishikosh.egranth.ac.in
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Mechanical composition
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Experiment 1: Assessing the impacts of elevated CO2and N levels on yield and nutrient uptake in rice
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Temperature Gradient Tunnels (TGT)
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Free Air Carbon dioxide Enrichment (FACE)Facility
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Site
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krishikosh.egranth.ac.in krishikosh.egranth.ac.in
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Phenological traits and plant height
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Analysis of variance and differences among wheat varieties released in different years in India
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Estimation of total N% of wheat grainsand straw
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Chlorophyllcontent
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Root length (cm) and Root weight (mg)
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Coleoptile length(cm)
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Stomata / cm2
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Leaf area index (LAI)
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Physiological parameters
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Normalized difference vegetation index (NDVI)
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Spike length (cm)
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Last node to spike length(cm)
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Peduncle length(cm)
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HarvestIndex
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Grain yield per plot (g)
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Biological Yield(g)
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1000 Kernel weight(mg)
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Number of grains per spike
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Number of productive tillers per meter
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Plant height (cm)
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Days to physiological maturity
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Days to heading
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Field observations
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Inoculum concentration
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STUDY AREA
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Tested individually
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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T cell frequencies(Post vaccination response)
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Decreased CD3 ζ chain expression on CD8 T cells in HBsAgpositive newborns
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T cell phenotypic distribution in HBsAgPositive, HBsAgNegative from HBsAg positive mothers and healthy newborns.
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Clinical characteristics of the subjects
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Healthy Controls
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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1X PBS
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Generation of polyclonal antibodies against GFP, APC and β-catenin
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Primary antibodies
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pGEX6P2-BPN (1-993 a.a)
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pET30a-GFP-FL (GFP antigen)
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pET30-b-APC Nde/Sca (1211-1495 a.a) (antigen for APC antibody)
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pET30a-β-catenin-FL (Antigen for -catenin antibody)
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GFP-β-cateninC-C (423-634 a.a) (ARM-C)
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GFP-β-catenin-C-N (151-423 a.a) (ARM-N)
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GFP-β-catenin-C (ARM)
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GFP-β-catenin- C (634 -781 a.a)
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GFP-β-catenin- a.a
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GFP-β-catenin T41A mutant
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Constructs made in the study
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GFP-β-catenin-FL
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github.com github.com
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passer
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Cell culture and drug treatment
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Commonly used Buffers/Reagents
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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IL-2 Bioassay
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IL-2 Bioassay
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IL-2 Bioassay
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IL-2 Bioassay
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Total Anti-Stm and anti-E coli antibody assay
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Bacteria
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Reagents
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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2 Bioassay
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.inThesis12
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Reagent
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Reagent
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Reagent
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Reagents
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Reagents
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Extraction and determination of ascorbic acid
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Standard curve of ascorbic acid
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Reagents
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Estimation of ascorbic acid
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Root and Shoot length
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ZnSO4
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Seeds and germination
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Phosphate buffered saline (PBS), 0.15M, pH 7.2
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Bacterial load in the spleens studied by cfu plating
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Expression of costimulatory molecules on the vaccines
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Animals
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Genomic DNA extraction
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Luria-Bertani (LB) medium
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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MN Blood group typing
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MN Blood group typing
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Reagents
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Reagents
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Isolation and culture of HUVECs
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Instruments used for the study
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MATERIALS
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Procedur
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Reagents
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Nitric oxide radical scavenging activity
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Procedur
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Reagents
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Hydroxyl radical scavenging assay
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Procedure
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Reagent
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Superoxide anion scavenging activit
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Procedure
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Reagent
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Total antioxidant activit
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Procedur
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Reagen
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Free radical scavenging activity
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In VitroStudies
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Chemicals
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Materials
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Material
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Materials
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Material
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Total alkalinity
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Dissolved oxygen and Biological oxygen demand
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Total dissolved solids
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Multi parameter analyzer
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MATERIALS AND METHODS
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PHYSICO-CHEMICAL PARAMETERS OF SEAWATER
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Characterization of embelin isolated from E.ribes
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. Extraction and isolation of embelin from E. ribes
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High Performance Liquid Chromatography (HPLC) analysis
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High Performance Thin Layer Chromatography (HPTLC) analysis
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. Thin Layer Chromatography (TLC) analysis
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Preliminary phytochemical screening (Ali, 1998; Evans, 2002)
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Microbial Contamina
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Foaming Index
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pH values
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Ash values
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Extractive value
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. Physico-chemical standardization
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Standardization of ethanolic extract of E.ribes (WHO, 1998; IndianPharmacopoeia, 1996)
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Preparation of ethanolic extract of E.ribes
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PLANT MATERIAL
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Estimation of Stevioside
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Extraction from the plant material
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Extraction from the plant material
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Extraction from the plant material
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Qmmtification of stevioside
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Estimation of Steviol
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Extraction from plant material
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Quantification of steviol
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Estimation of free aminoacids
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Estimation of soluble proteins
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Estimation of sugars
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Estimation of total phenols
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Determination of moisture
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Analytical methods
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The experimental part of the study was categorized into five sections for the convenience of reference viz. analytical, toxicological, molecular, biochemical and genomic quantitation.
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Plant material
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e5 tevia-the natural smeetenei
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Mean Somatotypes:
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Mean Somatotypes:
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Mean Somatotypes:
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Somatotype Categories:
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Range of Component Ratings:
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Somatocharts:
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Mean Somatotypes:
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SOMATOTYPE DISTRIBUTION OF URBAN SAMPLE
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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Per-cent Overlap
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Compogram
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F-and t-Ratios
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Chi-Square
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Somatotype Categories
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Comparative Statistics
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Somatotype Attitudinal Mean (SAM)
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Somatotype Attitudinal Distance (SAD)
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Somatotype Dispersion Mean (SDM)
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Somatotype Dispersion Distances (SDD)
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Somatoplot Coordinates
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Mean Somatotypes (5)
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Descriptive Statistics
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Statistical Analysis
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Central:
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Mesomorphic ectomorph:
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Endomorphic ectomorph:
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Ectomorphic mesomo~ph:
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Endomorphic mesomorph:
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Ectomorphic endomorph:
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Mesomorphic endomor~:
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Endomorph-ectom9££E:
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Mesomorph-endomorph:
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Mesomorph-endomorph:
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Balanced ectomorph:
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Balanced mesomorph:
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Balanced endomorph:
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SOHATOCHART
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Somatotyping Children
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Limitations of the Rating Form
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Third Component Rating
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Second Component Rating
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First Component Rating
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SOMATOTYPING
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Calf
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Biceps
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Femur
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Humerus
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Calf
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- md-1-md-17-md-1
- md-1-md-17-md-6
- md-1-md-29
- md-1-md-17-md-10
- md-1-md-14
- md-1-md-23
- md-1-md-26
- md-1-md-12
- md-1-md-17
- md-1-md-28
- md-1-md-17-md-9
- md-1-md-21
- md-1-md-16
- md-1-md-17-md-2
- md-1-md-17-md-13
- md-1-md-10
- md-1-md-17-md-7
- md-1-md-30
- md-1-md-17-md-11
- md-1-md-27
- md-1-md-24
- md-1-md-8
- md-1-md-6
- md-1-md-17-md-8
- md-1-md-7
- md-1-md-19
- md-1-md-17-md-3
- md-1-md-25
- md-1-md-9
- md-1-md-13
- md-1-md-17-md-4
- md-1-md-17-md-5
- md-1-md-31
- md-1-md-20
- md-1-md-11
- md-1-md-22
- md-1-md-17-md-12
- md-1-md-18
- md-1-md-15
Annotators
URL
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Primary culture of E. coli was grown in LB medium containing either ampicillin (Amp) and/or kanamycin (Kan) to final concentration of 100 j..tg/ml and 25 J..tg/ml respectively. Depending on the vector construct, antibiotics were used for expression of different proteins as described in Table 3.1. Medium was inoculated with 1 ml glycerol stock of E. coli and incubated overnight at 37 oc at 200 rpm.
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Preparation of primary culture of E. coli cells
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sg.inflibnet.ac.in sg.inflibnet.ac.in
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Blood was drawn from appropriate source into heparinised tubes. The blood sample was centrifuged at 4000 rpm for 15 min ( 4 °C). The supernatant was discarded, and the erythrocytes (pellet) were subsequently washed thrice with chilled isotonic buffer [0.01 M PBS (pH 7.4)] by centrifugation at 4000 rpm and 4°C for 15 min. The washed erythrocytes were lysed in water. The resultant red cell lysate was then dialyzed extensively against PBS (pH 7.4) at 4°C to obtain stripped hemoglobin (hemoglobin devoid of bound allosteric modulators like BPG). The stripped hemoglobin was then loaded onto a pre-equilibrated DE52 column (30cm x 15cm) after extensive dialysis against 0.05 M tris acetate buffer (pH 8.5). The protein was eluted from the column employing a linear gradient of 500 ml each of 0.05 M tris acetate (pH 8.5) and 0.05 M tris acetate (pH 7) at a flow rate of 50 ml/hour. The purified hemoglobin was estimated spectrophotometrically at 540 nm (molar extinction coefficient= 53236 cm-1/M) and stored at -70°C till further use.
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