therefore does not secrete glucose

Establishment Democrats are desperately hoping to avoid a reprise of 2016, when Mr. Sanders battled to the bitter end against Hillary Clinton; his posture enraged some in the party who later blamed him for stoking division among Democrats that led to her loss to Mr. Trump.

it is only after the other side has had a chance to put testimony to the test, through cross-examination, that it can be given the status of “evidence”

The app, which was developed in partnership with a contractor, will run on iPhone 8 devices provided by the bureau

essential functions

basic infrastructure

4200 rpm in Beckman J-4.2

physically present within the Schengen Area during the 14-day period preceding their entry or attempted entry into the United States.

PAM-independent loci were targeted using degenerate codons, thereby making it possible to modify any site in the genome

due to resource constraints, community-level tradeoffs exist between growth yield and resource acquisition, and that nutrient limitation affects community metabolism and reduces growth yield.

Subsurface drip irrigation provides the ultimate in water use efficiency for open-field agriculture, often resulting in water savings of 25-50% compared to flood irrigation

trace contaminants such as heavy metals in the upper horizons may be accumulated, which may eventually lead to deterioration of soil and groundwater quality and affect the sustainability of land-based disposal of effluent

Eggplant yield under treated effluent was twice the average eggplant production under fresh water irrigation using conventional fertiliser application in Jordan

included bacteria belonging to the Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria classes of proteobacteria

zygotically inducible

After purification, more than 30% of the bacterial cells in the primary soil were extracted

In situ probes using fluorescence and infrared spectroscopy are also available on the market and have been extensively studied

it is assumed that the decay modifies the growth multiplicatively as a function of the time

Typically, double terminators are used to stop transcription. Because these parts can be up to ∼168 bp of DNA, this use of double terminators can lead to homologous recombination when used at multiple locations in a design

In E. coli, homologous recombination occurs most frequently when there is a contiguous stretch of sequence >25 bp

Exposure to short chain-length AHLs resulted in a decrease in the abundance of different taxa than exposure to higher molecular weight AHLs

cognizable offence

Don't see the graphic above? Click here.

the leakiness and inducibility of the ligand-inducible LacI repressor mutants have been quantified and classified

One return New York-to-London transatlantic flight delivers roughly the same exposure (0.16 mSv) as a nuclear power worker receives in a year (0.18 mSv)

only

hydro-electric dams are one of the cheapest ways that poor nations can gain access to reliable electricity

opponents of hydro-electric dams and flood control claim those technologies

not the first time those who are most alarmist about an environmental problem have been most opposed to solving it.

large dams,

nuclear weapons have contributed to the Long Peace since World War II,

produced from abiotic photochemical reduction of Fe(III)-OM, thus closing a cryptic iron cycle under photic conditions

Fe(II) concentrations and cell abundances in R. ferrooxidans SW2 growth experiments

Abiotic photochemical reduction of Fe(III).

Fe(II) concentrations were determined anoxically using the ferrozine assay

were placed horizontally under a 40-W incandescent light bulb

2 mM FeCl2

Oxidation of Fe(II) by R. ferrooxidans SW2

guarantees that cells are actively metabolizing but no cell growth is possible, and that therefore, changing cell numbers does not influence the quantification of metabolic rates.

there was no Fe(II) oxidation in the abiotic controls

right-wing paramilitaries

Commerce follows theland and sea routes of the earth, going to whatever country hasany thing to exchange, be it a monarchy or a republic. Let us bein union with the whole world and not with just a part of it, notwith one part against another.

After many generations in the lab, mice from the desert consumed significantly less water than mice from other localities, indicating that this difference has a genetic basis.

all the polling evidence says that America is basically a center-left nation

is someone who plays right into that strategy, by declaring that he is indeed a socialist.

But were big banks really at the heart of the financial crisis, and would breaking them up protect us from future crises?

same workers in Spain are less equipped to compete for Manufacturing jobs

you might as well get the most productive workers for your “buck”, right?

Don’t hate corporations for playing to win — they don’t make the rules, you do.

you can’t ever stop because one correction will undo all your spending

wouldn't you be a fool for not using that move?

The researchers from the earlier, statistically significant, study

The host range for three of the plasmids (pBF1, pB7, and pB9) was further investigated by the GFP method described above

Consequently, the plasmid donor cells do not express gfp

you can take a screen clipping to create an image that combines and keeps items together

VLPs were discriminated from bacteria or detritus based on pixel dimensions: the pixel area of the smallest known bacterium was established as a maximum cutoff; all objects smaller than this were counted as VLPs.

is similar

Modeled trends of soil bacterial carrying capacity and diversity are compared to empirical observations

soil becomes sufficiently dry, almost all aqueous habitats are physically isolated and might contain only a few species

David Robert Grimes, an Irish science writer who has published research showing homeopathy to be ineffective

In biology there’s rarely things that happen for no reason

teach science to a general audience

persist on inanimate surfaces like metal, glass or plastic for up to 9 days

For n < 5 we recommend showing the individual data points.

hypothesized that any single quorum-sensing signal should only induce prophages within a small subset of closely related host bacteria

scientific laboratories create an amazing amount of plastic waste, consume large amounts of water, create risks from hazardous chemicals and use significantly more energy

plated out on warm agar plates

electroporation buffer (680 mM sucrose, 7 mM K2HPO4, pH 7)

idyllic conditions

in a pure socialist world everything would be needed to be controlled by the government

Most socialists, however, tend to find the profit motive problematic.

socialists do not support capitalism, meaning they want workers to control the means of production

The main priorities of different ELN features

whether shared systems of signalling synthesis and degradation

a single-tube, single-step PCR, performed in <2 hours from setup to transformation

Rice alumni with accounts on the Alumni Portal are automatically allowed to access a few of the university's licensed electronic resources through the EZproxy server

Bloom filter

if applied to raw sequence data they would only find matches completely contained within a single read.

our use case, where each new microbial dataset brings new variation

input data

Transmitting the same power using a higher voltage and lower current loses less of power over the length of the cable. This is more efficient and why the main power grid is hundreds of volts and not 5V.

obtained transcriptional measurements from mid-exponential-phase cultures

mobilizable plasmids, possess their own transfer origin and encode a relaxase but need the help of a conjugative plasmid for their transfer from a donor to a recipient

Plasmid pBBR1 thus appears to be a new member of this group, even though it resides in gram-negative bacteria and does not replicate via a rolling-circle mechanism

bind the transposon DNA at the flanking inverted repeat sequences, excise the transposon, and paste it into a random TA dinucleotide on a target DNA

J23119 is the "consensus" promoter sequence and the strongest member of the family

2-25 ug/mL

100x concentration of the initial culture.

J23100 2547

stationary-phase cultures grown in rich medium could be refrigerated for more than 1 month without substantial loss of viability

transformation efficiencies were sufficient for routine construction of plasmids from multiple fragments

Plasmids with the RK2 replicon could also replicate but were successfully delivered only via conjugation

A spacer could compensate for any steric hindrance effect on recruiting the transcriptional machinery at the promoter of the next module in the assembly line; such an effect could be induced by the supercoiling that results when the transcription of the previous module is taking place. However, there is no direct evidence that this may cause a problem, and therefore the argument in favor of using a spacer remains speculative.

As his ideas have permeated the rest of the field, Sanders’s own candidacy appears less radical—or at least less exceptional.

. If you are agreeable, you care about your family, your community, society, your country, more than you care about yourself. And if you are disagreeable, then you are a little bit more egotistical.

do: deregulate, and fast

Gujarat often produced growth faster than the national average, fewer regulations, better infrastructure and less corruption

We are taught that we should be defined by the things other people find impressive, not by the things that bring us joy

Get good at knowing what your friends and fans like to see and interact with

he rarely posts status updates and I post pretty frequently and often get good conversations going in the comments.

Calling out Uncle Bot’s past vote for Trump threatens his ego and encourages him to double down on his support for the president. Self-esteem is a fundamental human need. If you want people to change their opinion, bolster their ego and help them save face

Fully explore the other person’s perspective before sharing your own to ensure you understand where they are coming from. As with all questions, follow-up inquiries should be nonjudgmental, open-ended and curious.

The concentration of GFP in the sample had been measured using a nanodrop and was ~120μM

The fundamental issue surrounding bacterial culture growth measurements is that an absorbance spectrophotometer is being asked to determine light scattering caused by particulates in suspension. In this case, transmittance is not related to absorbance in the classical sense. Under normal true absorbance conditions, spectrophotometers can be comparable to one another because the sample actually absorbs electromagnetic energy. In the case of a reduction of transmittance caused by light scattering, readings are very dependent on the optics of a specific spectrophotometer as well as the cell type in suspension

generally accepted extinction coefficients for nucleic acids are: • Double-stranded DNA: 50 • Single-stranded DNA: 33 • RNA: 40

mCherry2 might have decreased cytotoxicity in E. coli.

After all, the rise in antibiotic resistance doesn’t mean that we shouldn’t have invented antibiotics at all.

If a corporation wants to use a gene drive to “cancel out” the herbicide resistance that some weeds have now developed, would that really benefit the planet — or just the corporation that can now sell more of the herbicide that caused the problem in the first place?

With its ability to create powerful changes invisibly, genetic engineering can feel eerie to even the most rational of us

Do we really want the process of scientific research and technology to become democratic — one in which fundamental decisions about public health, like vaccines and vector-control measures, are put up for a vote?

the lay public’s view is, once you say the person is a scientist, they must know everything.”

and hand control to Big Ag

s a general idea that these groups aren’t scientific, so their arguments are less valid

activist groups are more likely to tap into unconscious values and emotions — like using the term “Frankenfoods” to describe G.M.O.s

a misleading focus on “high-profile savior applications” like anti-malarial and conservation efforts

compared gene drives to the atomic bomb and accused researchers of using malaria as a Trojan horse to cover up the development of agricultural gene drives for corporate profit

Local and national governments would work with regulatory organizations like the United Nations and the World Health Organization,

Their financial interest in the intellectual property and their regulatory interest in making sure these products were able to come to market got conflated with the science, so nobody was willing to trust the kind of research they were doing. The end result was that all G.M.O. research got tainted.”

the technology was controlled primarily by the global agricultural giant Monsanto

struck most people as vaguely creepy

a weapon — say by sabotaging the pollinators that support agriculture, or by altering the genes of innocuous wild insects so they could transmit disease

Could a gene drive stop one virus only to open the way for another, more virulent one? Could it jump from one species to a related one? What would be the environmental effects, if any, of altering the genes of entire species? How about eliminating a species entirely?

Besides combating malaria, gene drives could be used to alter, or even eliminate, other disease-causing insects, from the sand flies that transmit leishmaniasis to ticks that carry Lyme disease in the United States.

power of the people is not only limited to the right of vote, but it extends further and provides us the right to participate in a democratic system by observing, suggesting and discussing, contemplating, questioning, negotiating and dissenting its system or function in various circumstances

CES (combinatorial enhancer solution). 5X CES = 2.7 M betaine, 6.7 mM DTT, 6.7% DMSO, and 55 ug/ml BSA

capable of checking multiple annealing temperatures at a time

increasing the magnesium concentration, which improves amplification

but the 5’ end is more important

relative granulocity/internal complexity (Side Scatter - SSC)

relative size (Forward Scatter - FSC)

We must stop building new nuclear power plants, and find a real solution to our existing nuclear waste problem.

More broadly, we hope that others, especially the hundreds of new startups in synthetic biology, consider sharing pre-competitive ideas, methods, tools, etc. We think the community will be stronger as a result.

we usually need 400-1200 colonies, it’s still easier and faster to do this manually than by colony picker. Also, colony pickers typically cost >$100K and don’t seem appropriate for our scale. 

manually analyzing the data was about as painful as waiting for the results

OCTOPUS, a light-weight, cost-effective, and robust method for full-plasmid sequence verification using next-generation sequencing

Biosensing intracellular concentrations of metabolites can provide an efficient high-throughput screen of hyperproducing strains.

Chemical inducers such as carbon sources, metabolites, and antibiotics, environmental signals such as light, pH, oxygen, or temperature, and autoregulatory quorum-sensing molecules can all be sensed by TFs

It boils down to priors: do we have reasons to expect large variance in opaqueness? Do we have reason to expect low variance in opaqueness?

A final word: when we do not understand something, it does not look like there is anything to be understood at all - it just looks like random noise. Just because it looks like noise does not mean there is no hidden structure.

because the receptor both activates and deactivates the internal signal, its concentration cancels out in the equilibrium expression

we were far in advance of Westerners in science and technology at that time.  Today we are far behind them, so what happened?  Why did we not have an Industrial Revolution?  Why did we lag behind?  This is known as Needham’s question or Needham’s Grand Question

In India, on the other hand, there is tremendous diversity, because whichever group of immigrants came into India brought in their own culture, their religion, their language etc.

Nevertheless, fierce Carter administration pressure mounted for him to abdicate.

Today, over 40% of the world’s people speak a mother tongue descended from the language of those nomads of the steppe.

If we reunite, we can pool in our resources and overcome these problems in 15-20 years

they do not want another China

communalism is an artificially-created phenomenon, and can easily be suppressed by a strong secular government.

Interactions between Mycobacterium tuberculosis (Mtb) and human (players) are often included in the Mtb’s strategies to invade host responses, to replicate and persist within the host,

Borate, which is present in LB and SB, causes reversible crosslinks with DNA, reducing the separation of larger DNA fragments unless low % gels are made of high-strength agarose

use of the strong UAAU signal in highly expressed genes and for the occurrence of the weaker UGAC signal at several recording sites.

suggestive of protein extension, especially at UGA codons, which rely exclusively on the reduced function RF2 variant of the K-12 strain for termination

Since capitalists make money from every hour of workers’ labor, they will get increasingly rich over time, while workers won’t because they’re too busy making money for capitalists

the rich pocket, rather than reinvest, their tax cuts

should reject the influence of both liberal capitalism and communism, ideas that inspired the revolutionary slogan "Neither East, nor West – Islamic Republic!"

Religious conflict in any form is often far more impervious to pragmatic solutions, and can prove more dangerous than national conflicts.

A single transformant may have a mutation at a low level that will eventually sweep through the population

promoter mutations remove the metabolic load at both the transcriptional and translational levels

in the normal MG1655 strain that does not overproduce LacI

a few simple design principles: high expression of genetic circuits comes with the cost of low evolutionary stability, avoid repeated sequences, and the use of inducible promoters increases stability. Inclusion of an antibiotic resistance gene within the circuit does not ensure evolutionary stability.

Engineering as a practice has not, however, been successful in such endeavors as controlling the weather or avoiding natural disasters, due largely to the scale, complexity and uncertainty inherent to those problems. So we must ask: is the conventional paradigm of engineering appropriate for biology?

CRISPRi-ME prevents evolutionary failure of a burdensome plasmid in E. coli.

SDS in loading buffer may contribute to band smearing in precast GelRed® gels. If this occurs, we recommend using the post-staining protocol

50 μg/ml RNase (ThermoFisher #EN053) freshly added. 2 ml of lysis buffer

deep-well plates, require a faster shaking speed for proper aeration

Composition of H15 growth medium

valorization of waste chemicals accumulating in industry is one of the pillars of the circular economy and the 4th Industrial Revolution

we recommend calibrating OD using serial dilution of silica microspheres, which readily produces highly precise calibration (95.5% of teams having residuals less than 1.2-fold), is easily assessed for quality control, and as a side effect also assesses the effective linear range of an instrument.

Bacteria are less likely to develop resistance against a pro-drug as it becomes active only after getting inside the bacteria. The active components formed from the pro-drug are potent and short-lived, thus not giving the bacteria sufficient time to develop resistance.

which ends up being much less efficient