June did this, June did that, she savedmoney and helped clean the house and cookedand Connie couldn't do a thing, her mindwas all filled with trashy daydreams.
I can’t imagine how stress Connie would be if she’d to heard those kind of salty remarks that keep compare her with her sister.
"Stop gawking at yourself. Who are you? You think you're so pretty?" she would say.
Her mother used the word “gawking”, I think it’s kind of impolite to her daughter. Her mother seemed to be irritated to her that she had to used such an indecent word.
Her mother, who noticed everything and knew everything and whohadn't much reason any longer to look at her own face, always scolded Connie about it.
It’s interesting here because it’s impossible in real life that one can know everything about the other. But the author made Connie’s mother be a person who omniscient about her daughter.
It depends in my opinion. Many variables with this company and what you seek as well as preferences. Do you want a three bank typewriter? I would go with an Erika 2 for a three bank typewriter. Do you want a more modern typewriter? Perhaps an Erika 10 is a good choice. Do you want a certain font? Maybe Fraktur? (Good luck finding one for cheap however) Erika 5 would be a good choice for fonts maybe. Do you want a rare and collectable model? Go with an Erika 20. They have basket shift too. I would personally avoid the newer models, such as the Erika 50. They aren't great in my preference, but you decide! There are many different models for different people, but the main company that produced Erikas, which is Seidel & Naumann, also created the "Ideal" brand of typewriters. S&N is responsible for a lot of typewriters.
https://www.reddit.com/r/typewriters/comments/1mqcu0f/erika_typewriters_i_am_looking_to_potentially_get/<br /> via LeSwiss1886
From Inner Work to Global Impact
for - program event selection - 2025 - April 2 - 10:30am-12pm GMT - Skoll World Forum - From Inner Work to Global Impact - Stop Reset Go Deep Humanity / cosmolocal - LCE - relevant to - event time conflict - with Building Citizen-Led Movements - solution - watch one live and the other recorded
meeting notes - see below
ANNIKA: - inner work helps us stay sane dealing with the chaos in our work - healing is not fixing - hope is a muscle, go to the "hope gym" - not just personal but collective
EDWIN: - inner WORK - constant, continuous work - how do you scale these things? Is it wrong term to use? Mechanistic? - how do we move to global impact? We don't know yet
LOUISE - inner work saved my - orientate inside away from trauma architecture - colonized and colonizer energies - they longed to be in union - be with all parts of myself - allow alchemy on the outside to the inside - liberate myself from my trauma structures and unfold myself - we cannot be a restorer unless we do that inner work - systeming - verbalizing / articulating it - we are all actors in creating the system - question - where am i systeming from? - answer - I am an interbeing - Am i systeming from the interbeing space or the trauma architecture space? - Where am I seeding from? What energy do I put into my work? - system is not concrete and fixed but fluid - fielding - bringing different human fields together - I can work with hatred and rage on the inside and transmute it so that I don't add to it on the outside
JOHN: - stuck systems and lens of trauma can help us get unstock - 70% of people have experienced trauma - trauma is part of the human experience - people make up systems - so traumatized people makes traumatized systems - fight, flight and freeze happens at both levels - at system level, its fractally similiar - disembodied from wisdom - in state of survival and fear - fixing things - until we deal with the trauma in the people, we will continue to have traumatized systems - More work won't help if it's coming from traumatized people
EDWIN - incremental change - something holding us back - built upon these traumas - Economic metrics are out of touch with how the trauma affects systems - Journey - awareness first, then understanding and inner transformation and finally change - Discussion with funders - most are still stuck in old paradigm of metrics, audits, etc - this comes with trauma because we have no trust on who is on the other side - a big part of the system is built on mistrust, creates more gaps between us - need to become anti-fragile
ANNIKA - Funders have lack of trust because inner work hasn't been done on both sides - As a funder, we really try to create a space of trust - Think of the language we use to be inclusive - How do we make inner work a part of the operating system of how we work? - We looked at 500 mental health organizations over the years - It's so urgent now that we align our work
EDWIN - We have a lot of half-formed thoughts - It's very complex and nobody has cracked it - We have a phrase at Axum that we move at the speed of trust - To do something different, they need to trust you - When I think of the discussions I've had with heads of states and CEOs, these meaningful inner ideas are not often brought up
LAURA - When there's no trust, even if there is no danger, the trauma is still brought up - We need to shift our lens on trauma and become aware of when trauma emerges - quote - inner condition of the intervener determines the success of intervention - Bill O'Brien
LOUISE - I work a lot with nervous system and body system - We need small changes in our nervous system - If I try to do something big, I can re-traumatize myself - We also have a collective nervous system - Restore love to all parts of your system first - Make friends with trees to seed actions from union
JOHN - Become aware of my own trauma triggers - When we see an outsized reaction, we can guess that person is undergoing personal trauma - A settled body settles bodies - If we are calm, it helps calm others
LAURA - Feel where we don't feel grounded, where we shame ourselves, feel compassion there
QUESTIONS - See below
mushrooms and ayuahuasca - is it helpful?
A lot of women forget the feminine energy to climb the ladder and get sick?
backlash - feels like white men were being pushed to do work they weren't ready to do so now reclaiming their comfortable traumatized space
how early do we start to teach this knowledge?
How do organizations hold space for the enormous trauma that the US govt is manufacturing. We need to build this practice into organizations to help deal with the onslaight
Youth are so hungry for being in the presence of others who are wise, compassionate. We can't move faster than the speed of trust but it needs to become accessible.
ANSWERS - See below
LOUISE - Organizations have a huge role to play at this time - We want to reconfigure and transform the trauma - Deep forming teams in organizations to help transform - Trauma fields want to come through human nervous systems to transform - We are both feminine and masculine and the masculine wounding is very important and needs to find the feminine - We cannot go away by ourselves to heal from patriarchy, colonialism energies
ANNIKA - In terms of how we fund, can we fund differently? We need to fund these spaces
EDWIN - I sit on board of Wellbeing project - changemakers go through burnout - how do we prevent this and create a container that can sustain them? - Weve brought 20,000 people in summits who have affected 3 million people. Please come to the Hurts summit in Czec and Wellbeing project - When pendulum swings back from individual space, we should be like a spiral
JOHN - In systems change spaces, trauma is seldom spoken of. - Systems work will not work if we ignore trauma - This is critical
LOUISE - Arundhati Roy - Another world is not only possible but is on its way. On a quiet day, I can hear it breathing.
Creative Tensions: Collaboration, Compromise, and Convict
for - program event selection - 2025 - April 3 - 10:30am-12pm GMT - Skoll World Forum - Creative Tension: Collaboration, Compromise and Conviction - Stop Reset Go - TPF - LCE - relevant to - event time conflict - with Aligning Profit and Purpose
Aligning Profit and Purpose
for - program event selection - 2025 - April 3 - 10:30am-12pm GMT - Skoll World Forum - Aligning Profit and Purpose - new portmanteau - greentruthing - opposite of greenwashing - Stop Reset Go - Deep Humanity - TPF - LCE - Greentruthing vs greenwashing - relevant to
Philanthropy at a Crossroads: Can We Fund
for - program event selection - 2025 - April 2 - 10:30am-12pm GMT - Skoll World Forum - Philanthropy at a Crossroads: Can we Fund at the Speed of Impacts? - Fellowship of the Sacred Commons - LCE - relevant to - event time conflict - with Building Citizen-led Movements - solution - watch one live and the other recorded - funding the commons
Building Citizen-Led Movements to Reshape Civic Life
for - program event selection - 2025 - April 2 - 10:30am-12pm GMT - Skoll World Forum - Building Citizen-ed Movements to Reshape Civic Life - Stop Reset Go - TPF - LCE - Building Citizen-Led Movements - relevant to
Oh stay, three lives in one flea spare,
Speaker essentially says to "spare the life of the flea as it has both their blood."
conventional rehab programs have very poor success rates usually people relapse anywhere from 2 to 10 times it's you know it's a re revolving door phenomenon
> for - stats - addiction - rehab relapse - 2 to 10 times - addiction - rehab - revolving door
global coding class, which is about 34 million digital nomads right now and maybe 10 million with a crypto wallet. Again, they're not rooted. They're rootless, and they should be root-full.
for - stats - 2025 - digital nomads - 34 million - with crypto - 10 million - rootless - SOURCE - Youtube Ma Earth channel interview - Devcon 2024 - Cosmo Local Commoning with Web 3 - Michel Bauwens - 2025, Jan 2
A shared car association, every shared car replaces 9 to 13 private cars for the same amount of travel freedom, point to point. You don't lose any freedom like you would in public transport. It's just like a neighborhood shares a dozen cars. 95% of the cars are in the garage at any time.
for - example - efficacy of mutualisation - transportation - cars - SOURCE - Youtube Ma Earth channel interview - Devcon 2024 - Cosmo Local Commoning with Web 3 - Michel Bauwens - 2025, Jan 2 - stats - mutualisation - transportation - cars - 1 car can replace 13 - car is parked most of the time - 10% of existing cars doubles our requirement - SOURCE - Youtube Ma Earth channel interview - Devcon 2024 - Cosmo Local Commoning with Web 3 - Michel Bauwens - 2025, Jan 2
le dialogue social 00:26:44 n'occulte pas non plus les élèves les syndicats d'élèves sont plutôt des associations qui peuvent se constituer librement mais qui doivent être autorisés par le chef par le chef d'établissement et le conseil d'administration pour pouvoir exercer 00:26:57 leur activité au sein des lycées j'en revois à l'article R 511-9 du code d'éducation la liberté de réunion des élèves est prévue et encadrée aux articles 00:27:11 l511-2 et r51-10 du code d'éducation ainsi que leur liberté d'expression qui est consacrée elle à l'article R 511-8 si le chef d'établissement doit 00:27:24 permettre aux associations d'élèves de jouir de leurs droits et de leur donner quelques é là encore boîte au lettres panneau d'affichage il doit surtout savoir qu'il est garant du fait que l'objet comme l'activité de 00:27:36 l'association n'est ni politique ni religieux et doit être compatible avec les principes du service public de l'enseignement le tout dans le respect du code pénal il en va de l'ordre public 00:27:48 scolaire et par conséquent d'un dialogue social apaisé
Investigativer Hintergrundbericht zum Aufbau von LNG-Kapazitäten durch Russland in der Arktis. Das Projekt Arctic LNG 2 ist nicht nur klimaschädlich, sondern auch katastrophal für die lokalen Ökosysteme. Europa bezieht mehr LNG aus Russland als vor der Invasion der kompletten Ukraine. Russland nutzte auch 2023 westliches Knowhow und trotz der Sanktionen gelieferte Ausrüstung vor allem der deutschen Firma Linde. https://www.derstandard.at/story/3000000191618/wie-westliche-konzerne-ein-russisches-prestigeprojekt-auch-nach-kriegsbeginn-ermoeglichten
Instance methods Instances of Models are documents. Documents have many of their own built-in instance methods. We may also define our own custom document instance methods. // define a schema const animalSchema = new Schema({ name: String, type: String }, { // Assign a function to the "methods" object of our animalSchema through schema options. // By following this approach, there is no need to create a separate TS type to define the type of the instance functions. methods: { findSimilarTypes(cb) { return mongoose.model('Animal').find({ type: this.type }, cb); } } }); // Or, assign a function to the "methods" object of our animalSchema animalSchema.methods.findSimilarTypes = function(cb) { return mongoose.model('Animal').find({ type: this.type }, cb); }; Now all of our animal instances have a findSimilarTypes method available to them. const Animal = mongoose.model('Animal', animalSchema); const dog = new Animal({ type: 'dog' }); dog.findSimilarTypes((err, dogs) => { console.log(dogs); // woof }); Overwriting a default mongoose document method may lead to unpredictable results. See this for more details. The example above uses the Schema.methods object directly to save an instance method. You can also use the Schema.method() helper as described here. Do not declare methods using ES6 arrow functions (=>). Arrow functions explicitly prevent binding this, so your method will not have access to the document and the above examples will not work.
Certainly! Let's break down the provided code snippets:
In Mongoose, a schema is a blueprint for defining the structure of documents within a collection. When you define a schema, you can also attach methods to it. These methods become instance methods, meaning they are available on the individual documents (instances) created from that schema.
Instance methods are useful for encapsulating functionality related to a specific document or model instance. They allow you to define custom behavior that can be executed on a specific document. In the given example, the findSimilarTypes method is added to instances of the Animal model, making it easy to find other animals of the same type.
methods object directly in the schema options:javascript
const animalSchema = new Schema(
{ name: String, type: String },
{
methods: {
findSimilarTypes(cb) {
return mongoose.model('Animal').find({ type: this.type }, cb);
}
}
}
);
methods object directly in the schema:javascript
animalSchema.methods.findSimilarTypes = function(cb) {
return mongoose.model('Animal').find({ type: this.type }, cb);
};
Schema.method() helper:javascript
animalSchema.method('findSimilarTypes', function(cb) {
return mongoose.model('Animal').find({ type: this.type }, cb);
});
Imagine you have a collection of animals in your database, and you want to find other animals of the same type. Instead of writing the same logic repeatedly, you can define a method that can be called on each animal instance to find similar types. This helps in keeping your code DRY (Don't Repeat Yourself) and makes it easier to maintain.
```javascript const mongoose = require('mongoose'); const { Schema } = mongoose;
// Define a schema with a custom instance method const animalSchema = new Schema({ name: String, type: String });
// Add a custom instance method to find similar types animalSchema.methods.findSimilarTypes = function(cb) { return mongoose.model('Animal').find({ type: this.type }, cb); };
// Create the Animal model using the schema const Animal = mongoose.model('Animal', animalSchema);
// Create an instance of Animal const dog = new Animal({ type: 'dog', name: 'Buddy' });
// Use the custom method to find similar types dog.findSimilarTypes((err, similarAnimals) => { console.log(similarAnimals); }); ```
In this example, findSimilarTypes is a custom instance method added to the Animal schema. When you create an instance of the Animal model (e.g., a dog), you can then call findSimilarTypes on that instance to find other animals with the same type. The method uses the this.type property, which refers to the type of the current animal instance. This allows you to easily reuse the logic for finding similar types across different instances of the Animal model.
https://en.wikipedia.org/wiki/Shmita
During shmita, the land is left to lie fallow and all agricultural activity, including plowing, planting, pruning and harvesting, is forbidden by halakha (Jewish law).
The sabbath year (shmita; Hebrew: שמיטה, literally "release"), also called the sabbatical year or shǝvi'it (שביעית, literally "seventh"), or "Sabbath of The Land", is the seventh year of the seven-year agricultural cycle mandated by the Torah in the Land of Israel and is observed in Judaism.
There are several occasions where the massebah is not associated with pagan worship. When the massebah is associated with the worship of Yahweh, the massebah is accepted as a valid expression of commitment to Yahweh.
Massebah for pagan worship: - Exodus 23:24 (https://hypothes.is/a/r3m5QmyDEe6SC8eLYcJE1Q) - Hosea 10:1 (https://hypothes.is/a/4PK2GGyDEe6wZg_r2YpVCA ) - 2 Kings 18:4 - 2 Kings 23:14
Massebah for worship of Yahweh: - Genesis 28:18 Jacob's pillow (https://hypothes.is/a/NF5p8Gx6Ee65Rg_J4tfaMQ)<br /> - Genesis 31:44-45 Jacob and Laban's covenant - Exodus 24:4 - Joshua 24:25-27
in violation of the demands of the covenant, the people of Israel erected sacred stones dedicated to other gods (Hosea 10:1). In their religious reforms, both Hezekiah (2 Kings 18:4) and Josiah (2 Kings 23:14) destroyed the sacred pillars which the people of Israel had dedicated to the worship of Baal.
During the establishment of the covenant between Yahweh and Israel, the people were commanded to destroy the sacred stones of the Canaanites, “You must demolish them and break their sacred stones (masseboth) to pieces” (Exodus 23:24).
In neighboring cultures in which both have oral practices relating to massebah, one is not just destroying "sacred stones" to stamp out their religion, but it's also destroying their culture and cultural memory as well as likely their laws and other valuable memories for the function of their society.
View this in light also of the people of Israel keeping their own sacred stones (Hosea 10:1) as well as the destruction of pillars dedicated to Baal in 2 Kings 18:4 and 2 Kings 23:14.
(Link and) Compare this to the British fencing off the land in Australia and thereby destroying Songlines and access to them and the impact this had on Indigenous Australians.
It's also somewhat similar to the colonialization activity of stamping out of Indigenous Americans and First Nations' language in North America, though the decimation of their language wasn't viewed in as reciprocal way as it might be viewed now. (Did colonizers of the time know about the tremendous damage of language destruction, or was it just a power over function?)
2. No hierarchy
Defang the hierarchy! Flattening all the management levels and leadership.
SUPPLEMENTARY DATA
AssayResult: -10
AssayResultAssertion: Normal
PValue: Not reported
Source Data
AssayResult: 74.18
AssayResultAssertion: Not reported
ReplicateCount: 2
StandardErrorMean: 6.49
Comment: Exact values reported in “Source Data” file.
Purification of PCR product
Multiple sequence alignment and phylogenetic analysis
HaemoglobinwasdeterminedbySahlimethod.HaemoglobinisconvertedtoacidhaematinbytheactionofHC1.Theacidhaematinsolutionisfurtherdilutedwiththeaciduntilitscolormatchesexactlythatofthepermanentstandardofthecomparatorblock.TheHbconcentrationisreaddirectlyfromthecalibrationcurve.
Haemoglobin(Hb)determination
hybridizations from biological replicates for each sample. Data was extracted with Feature Extraction software v 10.5 (Agilent) and normalizedwith GeneSpring GX v 11.0.1 (Agilent) software using the recommended Percentile shift Normalization to 75th percentile. Raw Data sets for this study are available at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=
GSE24267
Log-phase wild-type and Cgyps1∆cells were grown in YNB and YNB-pH 2.0 medium. After 1 h incubation, yeast cells were collected, washed and were stored in RNAlater at -80°C. These frozen samples were sent to Genotypic Technology Ltd., Bangalore(http://www.genotypic.co.in) whichprovides services of global gene analysis on Agilent platform. A 8x15k GE array comprised of 60mer oligonucleotidesfor a total of C. glabrata5503 genes was used wherein average number of replicates for each probe was three. Labeling was done in single color and data is the average of two
Microarray analysis
Alipophilic styryl dye,FM4-64,is a vital stain which istakenupby cells viaendocytosis through plasma membrane(Vida and Emr, 1995). Therefore, it fluorescesonly in live cells. Importantly, neitherfixed cells canbe stained with FM 4-64norcells canbe fixed afterFM 4-64staining. For vacuole staining, single colony of the test strain grown onYPD plate was inoculated in 10 ml YPD medium for overnight. 100 μlovernight culture was inoculated in fresh YPD medium and incubated at 30ºC for 3 hto obtain log-phase cells. C. glabratacells from 1 ml log-phase culture were harvested at 4,000 rpm for 5 minin a table top centrifuge. Supernatant was aspirated out,cells were resuspended in 50 μl YPD medium and 1 μl FM 4-64 (16 μM final concentration) was added.C. glabratacells were incubated in a 30ºC water bath for 30 min. 1 mlYPD medium was added and cells were harvested at 4,000 rpm for 5 minin a table-top centrifuge. After discarding supernatant,C. glabratacells were washed with fresh YPD medium and resuspended in 1 ml YPD medium. C. glabratacells were incubated at 30ºC for 90 min, washed with 1 mlsterile water and were resuspended in 50 μl YNB medium. Labeled C. glabratacells were observed underfluorescence microscope in red filter(730nm)
Stainingof C. glabratavacuoleswith FM4-64
DNA staining solution
Fixative
For cell cycle analysis by flow cytometry
Benzamidine
confirmed through PCR (by using primers SC11 and SC10) and squencing. Double mutant was complemented for DSF production by cloning whole rpfFgene of Xoo, cloned in HindIII and EcoRI sites of pHM1 (a broad host range vector for Xanthomonas) to get pSC6 plasmid. The resultant pSC6 plasmid was introduced into double mutant by electroporation
To obtain the insertional nonpolar mutant in the motA (encodes flagellar motor stator protein)andfliC (flagellin)genes, a 321 bp internal fragment of the motAgene and a 450 bp internal fragment of fliC containing the EcoRI and XbaI site were amplified using respective primer listed in Table 2.2. These fragments were cloned in pk18mob suicide vector, in which the lacZpromoter drives the expression of downstream gene (Schäfer et al., 1994; Windgassen et al., 2000), to obtain pRR1 and pRR2,respectively (Table 2.2). The resulting plasmid (pRR1& pRR2) was introduced into XooBXO43 strain by electroporation. Single Kmrrecombinants were selected on PSA plate containing kanamycin. Insertion of the pK18mob vector in motA andfliCgene was confirmed with PCR and sequencing. To further confirm the mutation in the flagellar genes, we did swimming motility assay on 0.1% peptone-sucrose agar (PSA). Swimming plate assay indicated that both motA and fliCmutant of Xoowas deficient in motility. Further, to obtain motAand fliC insertional knock out mutants in rpfFbackground, we cloned spectinomycin cassette obtained from pUC1318Ω plasmid, into the HindIII site of pRR1 and pRR2 plasmid to obtain pRR3 and pRR4. The resulting plasmid (pRR3& pRR4) were transformed in rpfF. Single specrrecombinants were selected on PSA plate containing kanamycin and spectinomycin. Insertion of the vector was further confirmed by PCR and sequencing. T2SSrpfFdouble mutant was constructed by transforming the plasmid with rpfF::Tn7Kanamycin cassette in the T2SS (xpsF) mutant background, and Kmrrecombinants were selected on PSA plates containing kan
amycin antibiotic and
Construction of mutants in X. oryzaepv. oryzae
Plan title - should this be the same as the grant title? Part of project metadata
http://www.bbsrc.ac.uk/documents/data-sharing-policy-pdf/ http://www.bbsrc.ac.uk/funding/apply/application-guidance/data-management/
Cindy A. Buckmaster: Animal research Is a labor of love for animals and people
This page was analyzed on May 5, 2017 and was found to have low virality and impact. While the content may be either true or false, it is nnot moving through the network in the way we see hoaxes or clickbait move.