48 Matching Annotations
  1. Jan 2024
    1. a kanamycin resistance gene known to be expressed in both Gram-negative and Gram-positive bacteria

      aphA-3 gene from Campylobacter coli. Ref 21, 25 ~ quoted

      A 1427-bp DNA fragment containing the kanamycin resistance gene, aphA-3, of plasmid pIP1433 from Campylobacter coli was inserted into a shuttle vector. Full expression of aphA-3 was obtained in Bacillus subtilis and in Escherichia coli.

  2. Oct 2023
  3. Aug 2023
  4. Jun 2023
  5. May 2023
    1. abnf Range: <unit>=<range-start>- Range: <unit>=<range-start>-<range-end> Range: <unit>=<range-start>-<range-end>, <range-start>-<range-end> Range: <unit>=<range-start>-<range-end>, <range-start>-<range-end>, <range-start>-<range-end> Range: <unit>=-<suffix-length>

    1. ```http GET http://localhost:50714/api/Car HTTP/1.1 User-Agent: Fiddler Host: localhost:50714 Range: x-entity=2-5

      HTTP/1.1 206 Partial Content Cache-Control: no-cache Pragma: no-cache Content-Type: application/json; charset=utf-8 Content-Range: x-entity 2-5/10 Expires: -1 Server: Microsoft-IIS/8.0 Date: Tue, 31 Jul 2012 19:00:19 GMT Content-Length: 447

      [{"Id":3,"Make":"Toyota","Model":"Yaris","BuildYear":2003,"Price":3750.0,... ```

    1. ```http GET /users

      200 OK Accept-Ranges: users Content-Range: users 0-9/200

      [ 0, …, 9 ] ```

      ```http GET /users Range: users=0-9

      206 Partial Content Accept-Ranges: users Content-Range: users 0-9/200

      [ 0, …, 9 ] ```

      ```http GET /users Range: users=0-9,50-59

      206 Partial Content Accept-Ranges: users Content-Type: multipart/mixed; boundary=next

      --next Content-Range: users 0-9/200

      [ 0, …, 9 ]

      --next Content-Range: users 50-59/200

      [ 50, …, 59 ]

      --next-- ```

      ```http GET /users?name=Fred

      206 Partial Content Accept-Ranges: users Content-Range: users 0-100/*

      [ 0, …, 100 ] ```

  6. Jan 2023
    1. ‘Running on Emptiness – The Pathology of Civilisation’John Zerzan (2002) All religions have problems with ‘unbelievers’, but that response is insignificant compared to their visceral hatred of ‘apostates’.

      !- Book Review : Free Range Activist !- Title : ‘Running on Emptiness – The Pathology of Civilisation’ !- Author : John Zerzan (2002) !- Website : http://www.fraw.org.uk/blog/reviews/023/index.shtml

      • All religions have problems with ‘unbelievers’, but that response is insignificant compared to their visceral hatred of ‘apostates’.
  7. Dec 2022
    1. We propose that the stricter of the safe and just target ranges for each variable should define the safe and just corridor (Figure 2). Furthermore, we propose to identify a spread of safe and just targets corresponding to different physical risk tolerances and different understandings of environmental justice.

      !- range : safe and just corridors - identify a range of safe and just corridors for society to choose - depending on different physical risk tolerances / environmental justice

  8. Jan 2022
  9. Nov 2021
    1. 𝚃𝚘𝚖 𝙻𝚊𝚠𝚝𝚘𝚗 💙. (2021, October 30). From the paper—Ventilation makes a big difference further away, but below 1-1.5m then you’d have to be in a gale to be safe! ✅DISTANCE if you can ✅VENTILATE - works even within 2m, but sadly not so much within 1-1.5m ✅PPE if you have to get close #COVIDisAirborne https://t.co/wYuWdG47He [Tweet]. @LawtonTri. https://twitter.com/LawtonTri/status/1454355692593328132

  10. Sep 2021
    1. A PFC assessment can be incorporated into an integrated riparian management process through a logical sequence of actions (figure 2). Figure 2. Recommended steps for managing riparian areas using an integrated process. After effectiveness monitoring has been done (step 6), initial objectives are validated and modified if necessary. After implementing adaptive actions, step 6 is repeated to monitor the effectiveness of those actions.Step 7: Implement adaptive actionsStep 6: Monitor and analyze the effectiveness of actions and update resource condition ratings (PFC)Step 5: Design and implement management and restoration actionsStep 4: Identify issues and establish goals and objectivesStep 3: Prioritize reaches for management, restoration, or monitoring actionsStep 2: Identify riparian resource values and complete additional assessmentsStep 1: Assess riparian area function using the PFC method • Identify assessment area and assemble an interdisciplinary team • Review existing information and delineate and stratify reaches • Determine the potential of the reach • Assess the reach and determine its functional rating (validate with monitoring data if necessary)Modifyobjectivesif necessaryMonitoradaptiveactions
  11. Apr 2021
    1. Sweetshrub grows best in average to rich, well-drained soil in anywhere from full sun to deep shade. It prefers some shade in hot summer afternoons and it will grow lankier and less dense in shade than in sun. It is tolerant of a wide range of soil textures and pH but prefers rich loams.  Plant it 3 to 5 feet from other shrubs to give it adequate room to grow.  It blooms in early spring before leaves emerge, with the leaves, and sporadically thereafter.  

      Very tolerant of shade or sun and most soils.

  12. Jan 2021
    1. RSF1010 is maintained at a copy-number of about 10-12 per chromosome in E coli, P. aeruginosa and S. enterica sv.Typhimurium (Frey and Bagdasarian, 1989)
    1. This work provides CasTn as a new method for host-independent, programmable, targeted DNA insertions to expand the genomic engineering toolbox.
  13. Dec 2020
    1. The pBHR1 plasmid is a derivative of the small (2.6-kb), mobilizable broad-host-range plasmid pBBR1, which was isolated from the gram-negative bacterium Bordetella bronchiseptica

      pBBR1

  14. Nov 2020
    1. Among the fully sequenced BHR plasmids, most are classified as the well-known incompatibility groups, such as IncP-1 (41 plasmids), IncW (5 plasmids), and IncU (4 plasmids), based on the backbone genes
  15. Oct 2020
  16. Aug 2020
  17. Jul 2020
    1. Why don't you allow a range without end, like (1..)? There are two advantages. First, we can write ary[1..] instead of ary[1..-1]. The -1 is one of the most I dislike in Ruby. It is very magical, ugly, redundant, and disappointing. I envy Python's ary[1:]. I know that ary.drop(1) is slightly fast, but too long for such a common operation, IMO. Second, we can write (1..).each {|n| ... }.
  18. Jun 2020
  19. May 2020
  20. Feb 2020
    1. The host range for three of the plasmids (pBF1, pB7, and pB9) was further investigated by the GFP method described above

      Does the host range of transfer go wider than the population where GFP is observed?

      This is my idea of distinctive overlapping categories of host range as venn diagrams.

  21. Jan 2020
    1. Plasmid pBBR1 thus appears to be a new member of this group, even though it resides in gram-negative bacteria and does not replicate via a rolling-circle mechanism
  22. Dec 2019
  23. Oct 2019
    1. RP4 has been shown to exhibit a very broad host range and has been demonstrated to efficiently transfer in activated sludge (22, 40), chicken manure (24), and the rhizospheres of various plants (38, 39).
  24. Jan 2019
  25. Oct 2016